Exam 18: Recombinant Dna and Biotechnology

Suppose the first three nucleotides of a palindromic restriction site are AGT.If the restriction site is six nucleotides long, its next three nucleotides will be _______.

The restriction enzymes that are most useful in generating recombinant DNA are those that

The restriction enzyme AluI produces blunt ends.From this we can conclude that

Recombinant DNA has been inserted into a mouse cell line.This is a process called _______.

For DNA to be considered recombinant DNA, it must be made in the laboratory and be derived from _______ source(s).

Which technique makes use of reverse transcriptase?

In the construction of an expression vector, a signal sequence

A signal sequence would most likely be used for

Refer to the figure showing how genomic libraries can be constructed. Of a mouse or yeast, in which organism would the difference in size between the cDNA library and the genomic library be larger? Explain your answer.

You are a researcher interested in getting a pig gene into the replicon of a bacterial host.Which method would you most likely use to get this gene to be part of the replicon?

What role do spacers play in the CRISPR-CAS9 system found in many bacteria?

The sequence 5ʹ-GATATT-3ʹ is not a palindromic restriction site.A mutation that would likely make it a restriction site would be from T nearest the 3ʹ end to _______.

Refer to the table showing results from an experiment in which researchers used CRISPR technology to knock out various genes from a fly.They then examined the developmental time (in days, with calculated means) and larval survival (percent, with calculated means) for each treatment.(Note: The "x" marks genes that have been knocked out.) Does gene A affect any of the traits examined? Explain your answer.

What was the original source of the green fluorescent protein?

Complementary single-stranded RNA

Bacteria are not particularly useful as hosts in studies of eukaryotic gene expression because they

In Cohen and Boyer's pioneering studies in recombinant DNA, what was the role of antibiotic resistance genes?

Refer to the table showing results from an experiment in which researchers used CRISPR technology to knock out various genes from a plant.They then examined plant stem height (in centimeters, with calculated means) and seed production (number of seeds, with calculated means) with each treatment.(Note: The "x" marks genes that have been knocked out.) What is the most likely role of the product of gene B?

Refer to the table.A gene might affect the length of bristles of a beetle.Using CRISPR technology, biologists have altered the sequence of this gene at three different nucleotides (765, 2901, and 4576) and have measured the length of bristles (in millimeters, with calculated means).In nature, most beetles have A at position 765, G at position 2901, and A at position 4576 (see beetle 1). Changing A to G at position 4576 will likely _______ the length of bristles.

A biologist tries to produce many copies of a particular gene using recombinant DNA technology.This process is called