Question 1

In the assay in the preceding figure, if the patient had rheumatoid factor

Accepted Answer

A) the test would be a false negative 
B) and high titers of the antibody to the antigen, the test would be a false negative 
C) the test results would be unaffected 
D) the test would be a false positive 
A) the test would be a false negative 
B) and high titers of the antibody to the antigen, the test would be a false negative 
C) the test results would be unaffected 
D) the test would be a false positive D

Question 2

Which two labeled immunoassays do not require instrumentation?

Accepted Answer

A) colloid immunoassay and chemiluminescent assay 
B) fluorescent immunoassay and optical immunoassay 
C) colloid immunoassay and optical immunoassay 
D) fluorescent immunoassay and a colorimetric enzyme immunoassay 
A) colloid immunoassay and chemiluminescent assay 
B) fluorescent immunoassay and optical immunoassay 
C) colloid immunoassay and optical immunoassay 
D) fluorescent immunoassay and a colorimetric enzyme immunoassay C

Question 3

Which of the following is a homogeneous assay?

Accepted Answer

A) a sandwich assay 
B) an immunocolloid assay 
C) a fluorescence polarization immunoassay 
D) a multiplex assay 
A) a sandwich assay 
B) an immunocolloid assay 
C) a fluorescence polarization immunoassay 
D) a multiplex assay C

Question 4

The presence of human heterophilic antibody is

Accepted Answer

A) difficult to predict 
B) the result of the patient's interactions with animals of the species used to make the antibody in the assay 
C) can cause false positive reactions in capture assays 
D) all of the above 
A) difficult to predict 
B) the result of the patient's interactions with animals of the species used to make the antibody in the assay 
C) can cause false positive reactions in capture assays 
D) all of the above

Question 5

For a small antigen with one epitope that is in the fluid phase, the best assay would be

Accepted Answer

A) a capture assay 
B) a competitive assay 
C) a direct immunofluorescent assay 
D) nephelometry 
A) a capture assay 
B) a competitive assay 
C) a direct immunofluorescent assay 
D) nephelometry

Question 6

Which of the following assays uses a trigger before the signal can be read?

Accepted Answer

A) a homogeneous assay 
B) a radioimmunoassay 
C) a fluorescent immunoassay 
D) a chemiluminescent assay 
A) a homogeneous assay 
B) a radioimmunoassay 
C) a fluorescent immunoassay 
D) a chemiluminescent assay

Question 7

The kit for the homogeneous assay for methamphetamine comes with methamphetamine linked to glucose-6-phosphate dehydrogenase, and antibody to the drug blocks the action of the enzyme when the patient's serum contains

Accepted Answer

A) the drug and there is more enzyme reactivity 
B) the drug and there is less enzyme reactivity 
C) low levels of the drug and there is less enzyme reactivity than without the patient's serum 
D) high levels of the drug and there is less enzyme reactivity than without the patient's serum 
A) the drug and there is more enzyme reactivity 
B) the drug and there is less enzyme reactivity 
C) low levels of the drug and there is less enzyme reactivity than without the patient's serum 
D) high levels of the drug and there is less enzyme reactivity than without the patient's serum

Question 8

In the homogeneous assay for methamphetamine, the kit comes with methamphetamine linked to glucose-6-phosphate dehydrogenase, and antibody to the drug blocks the action of the enzyme when the patient's serum contains

Accepted Answer

A) low levels of the drug and there is less enzyme reactivity than without the patients serum 
B) high levels of the drug and there is less enzyme reactivity than without the patient's serum 
C) the drug and there is more enzyme reactivity 
D) the drug and there is less enzyme reactivity 
A) low levels of the drug and there is less enzyme reactivity than without the patients serum 
B) high levels of the drug and there is less enzyme reactivity than without the patient's serum 
C) the drug and there is more enzyme reactivity 
D) the drug and there is less enzyme reactivity

Question 9

A indirect radioimmunoassay uses a different second antibody binding step because of the radiolabel but the same first antibody binding step as

Accepted Answer

A) an indirect enzyme immunoassay 
B) an indirect fluorescent immunoassay 
C) capture assay 
D) A and B 
A) an indirect enzyme immunoassay 
B) an indirect fluorescent immunoassay 
C) capture assay 
D) A and B

Question 10

In a sandwich assay,

Accepted Answer

A) the antibody is sandwiched between a capture antigen and a labeled antigen 
B) the antigen is sandwiched between a capture antibody and a labeled antibody 
C) either A or B 
D) none of the above 
A) the antibody is sandwiched between a capture antigen and a labeled antigen 
B) the antigen is sandwiched between a capture antibody and a labeled antibody 
C) either A or B 
D) none of the above

Question 11

Optical immunoassays are based on

Accepted Answer

A) a change in the color of light reflected from the surface when ag and ab are bound 
B) a change in color seen in a fluorescent immunoassay 
C) the use of immunochromatography 
D) chemiluminescence 
A) a change in the color of light reflected from the surface when ag and ab are bound 
B) a change in color seen in a fluorescent immunoassay 
C) the use of immunochromatography 
D) chemiluminescence

Question 12

In a competitive immunoassay, the

Accepted Answer

A) amount of signal is directly proportional to the amount of patient's antibody and can be determined from a standard curve 
B) amount of signal is inversely proportional to the amount of patient's antibody and can be determined from a standard curve 
C) amount of signal is proportional to the square of the patient's antibody concentration 
D) amount of signal is inversely proportional to the square of the antibody concentration 
A) amount of signal is directly proportional to the amount of patient's antibody and can be determined from a standard curve 
B) amount of signal is inversely proportional to the amount of patient's antibody and can be determined from a standard curve 
C) amount of signal is proportional to the square of the patient's antibody concentration 
D) amount of signal is inversely proportional to the square of the antibody concentration

Question 13

A western blot differs from a recombinant immunoblot because

Accepted Answer

A) the antigens on the nitrocellulose strip in the western blot have been blotted directly onto the blot but the antigens on the recombinant immunoblot are electrophoresed onto the blot 
B) because the antigens on the nitrocellulose strip in the western blot have been electrophoresed and then blotted onto the blot and the recombinant immunoblot the antigens are just pipetted onto the blot 
C) because in a western a direct EIA is performed and in a recombinant immunoblot an indirect EIA is performed 
D) because in a western an indirect EIA is performed and in a recombinant immunoblot an indirect EIA is performed 
A) the antigens on the nitrocellulose strip in the western blot have been blotted directly onto the blot but the antigens on the recombinant immunoblot are electrophoresed onto the blot 
B) because the antigens on the nitrocellulose strip in the western blot have been electrophoresed and then blotted onto the blot and the recombinant immunoblot the antigens are just pipetted onto the blot 
C) because in a western a direct EIA is performed and in a recombinant immunoblot an indirect EIA is performed 
D) because in a western an indirect EIA is performed and in a recombinant immunoblot an indirect EIA is performed

Question 14

The best name for the assay in the figure below would be

Accepted Answer

A) a capture enzyme immunoassay 
B) a double capture enzyme immunoassay 
C) a direct enzyme immunoassay 
D) an indirect enzyme immunoassay 
A) a capture enzyme immunoassay 
B) a double capture enzyme immunoassay 
C) a direct enzyme immunoassay 
D) an indirect enzyme immunoassay

Question 15

The presence of human heterophilic antibody

Accepted Answer

A) is difficult to predict 
B) results from the patient's interactions with animals of the species used to make the antibody in the assay 
C) can cause false positive reactions in capture assays 
D) all of the above 
A) is difficult to predict 
B) results from the patient's interactions with animals of the species used to make the antibody in the assay 
C) can cause false positive reactions in capture assays 
D) all of the above

Question 16

A indirect radioimmunoassay uses a different second antibody-binding step because of the radiolabel, but the same first antibody binding step is

Accepted Answer

A) an indirect enzyme immunoassay 
B) an indirect fluorescent immunoassay 
C) a capture assay 
D) all of the above 
A) an indirect enzyme immunoassay 
B) an indirect fluorescent immunoassay 
C) a capture assay 
D) all of the above

Question 17

HAMA

Accepted Answer

A) is human anti-mouse antibody 
B) can cause false positives in capture immunoassays in which monoclonal antibodies are used 
C) can cause false negative reactions in capture immunoassays in which mouse monoclonal antibodies are used 
D) A and B 
E) A and C 
A) is human anti-mouse antibody 
B) can cause false positives in capture immunoassays in which monoclonal antibodies are used 
C) can cause false negative reactions in capture immunoassays in which mouse monoclonal antibodies are used 
D) A and B 
E) A and C

Question 18

Which is most sensitive?

Accepted Answer

A) an Ouchterlony reaction 
B) an agglutination reaction 
C) a homogeneous labeled immunoassay 
D) a heterogeneous labeled immunoassay 
A) an Ouchterlony reaction 
B) an agglutination reaction 
C) a homogeneous labeled immunoassay 
D) a heterogeneous labeled immunoassay

Question 19

The reaction shown in the figure is

Accepted Answer

A) a sandwich immunoassay 
B) a capture immunoassay 
C) an indirect immunoassay 
D) a direct immunoassay 
E) A and B 
A) a sandwich immunoassay 
B) a capture immunoassay 
C) an indirect immunoassay 
D) a direct immunoassay 
E) A and B

Question 20

In a capture assay for IgM that reacts with rubella, an antibody to human IgM binds the patient IgM, rubella antigen from the kit is added, and then an enzyme labeled antibody to rubella is added. What would happen to this assay if the patient had rheumatoid factor but no IgM antibodies to rubella?

Accepted Answer

A) The patient would appear positive for IgM rubella antibodies unless bovine serum albumin was added as a nonspecific blocker. 
B) The patient would appear positive for IgM rubella antibodies unless nonfat dried milk was added as a nonspecific blocker. 
C) The patient would appear positive for IgM rubella antibodies unless human IgG that did not react with rubella was added as a specific blocker. 
D) The patient would appear negative for IgM rubella antibodies unless human IgG that did not react with rubella was added as a specific blocker. 
A) The patient would appear positive for IgM rubella antibodies unless bovine serum albumin was added as a nonspecific blocker. 
B) The patient would appear positive for IgM rubella antibodies unless nonfat dried milk was added as a nonspecific blocker. 
C) The patient would appear positive for IgM rubella antibodies unless human IgG that did not react with rubella was added as a specific blocker. 
D) The patient would appear negative for IgM rubella antibodies unless human IgG that did not react with rubella was added as a specific blocker.

Exam 7: Labeled Immunoassays

In the assay in the preceding figure, if the patient had rheumatoid factor

Which two labeled immunoassays do not require instrumentation?

Which of the following is a homogeneous assay?

The presence of human heterophilic antibody is

For a small antigen with one epitope that is in the fluid phase, the best assay would be

Which of the following assays uses a trigger before the signal can be read?

The kit for the homogeneous assay for methamphetamine comes with methamphetamine linked to glucose-6-phosphate dehydrogenase, and antibody to the drug blocks the action of the enzyme when the patient's serum contains

In the homogeneous assay for methamphetamine, the kit comes with methamphetamine linked to glucose-6-phosphate dehydrogenase, and antibody to the drug blocks the action of the enzyme when the patient's serum contains

A indirect radioimmunoassay uses a different second antibody binding step because of the radiolabel but the same first antibody binding step as

In a sandwich assay,

Optical immunoassays are based on

In a competitive immunoassay, the

A western blot differs from a recombinant immunoblot because

The best name for the assay in the figure below would be

The presence of human heterophilic antibody

A indirect radioimmunoassay uses a different second antibody-binding step because of the radiolabel, but the same first antibody binding step is

HAMA

Which is most sensitive?

The reaction shown in the figure is

In a capture assay for IgM that reacts with rubella, an antibody to human IgM binds the patient IgM, rubella antigen from the kit is added, and then an enzyme labeled antibody to rubella is added. What would happen to this assay if the patient had rheumatoid factor but no IgM antibodies to rubella?

Introduction to Immunology

Antibody

Antigens-Epitopes and Immunogenicity

Cellular Immunity

Complement Pathways

Agglutination and Precipitation Reactions: the Unlabeled Immunoassays

Serology Laboratory Math

Hypersensitivity Reactions

Autoimmunitysystemic

Autoimmunityorgan or Tissue Specific

Tumor Immunology

Hematopoeitc Malignancies

Immunology of Transplantation

Primary Immunodeficiency Diseases

Acquired Immunodeficiency Including Hiv and Aids

Hepatitis

Serology and Viruses Herpesviridae Family and Other Serologically

Bacterial Serology

Fungal Serology Parasitic Serology

Forensic Serology

Safety

Molecular Biology

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