Question 1

In serum protein electrophoresis, the protein that moves most quickly toward the anode is

Accepted Answer

A) albumin 
B) alpha 1 
C) alpha 2 
D) gamma globulins 
A) albumin 
B) alpha 1 
C) alpha 2 
D) gamma globulins A

Question 2

If a particular IgG molecule and IgM molecule have the same affinity, which would have a higher avidity?

Accepted Answer

A) IgG 
B) IgM 
C) Both would have the same avidity 
D) More data are needed to know the avidity 
A) IgG 
B) IgM 
C) Both would have the same avidity 
D) More data are needed to know the avidity B

Question 3

IgG, IgM, IgA, and IgE

Accepted Answer

A) can bind the same antigen 
B) have the same Fc region 
C) have different Fc regions 
D) can bind the same antigen and have the same Fc region 
E) can bind the same antigen and have different Fc regions 
A) can bind the same antigen 
B) have the same Fc region 
C) have different Fc regions 
D) can bind the same antigen and have the same Fc region 
E) can bind the same antigen and have different Fc regions E

Question 4

At pH 8.6, most serum proteins

Accepted Answer

A) have a negative charge that causes them to move toward the anode 
B) have a positive charge that causes them to move toward the anode 
C) have a negative charge that causes them to move toward the cathode 
D) have a positive charge that causes them to move toward the cathode 
A) have a negative charge that causes them to move toward the anode 
B) have a positive charge that causes them to move toward the anode 
C) have a negative charge that causes them to move toward the cathode 
D) have a positive charge that causes them to move toward the cathode

Question 5

A spleen cell and a myeloma cell are used to produce a monoclonal antibody. How do they help produce a monoclonal antibody?

Accepted Answer

A) The myeloma cell produces factors that help the spleen cell live longer. 
B) The myeloma cell and the spleen cell are fused to become one cell, a hybridoma. 
C) Two myeloma cells fuse with one spleen cell to become a hybridoma. 
D) Because the myeloma cell is defective, it can't make immunoglobulin, but it takes up just the genetic material from making immunoglobulin from the spleen cell and becomes a hybridoma. 
A) The myeloma cell produces factors that help the spleen cell live longer. 
B) The myeloma cell and the spleen cell are fused to become one cell, a hybridoma. 
C) Two myeloma cells fuse with one spleen cell to become a hybridoma. 
D) Because the myeloma cell is defective, it can't make immunoglobulin, but it takes up just the genetic material from making immunoglobulin from the spleen cell and becomes a hybridoma.

Question 6

Monoclonal antibodies are produced by cells that are hybrids of

Accepted Answer

A) pleen cells and skin tumor cells 
B) neutrophils and myeloma cells 
C) spleen cells and myeloma cells 
D) macroglobulinemia cells and myeloma cellsMatching Questions 
A) pleen cells and skin tumor cells 
B) neutrophils and myeloma cells 
C) spleen cells and myeloma cells 
D) macroglobulinemia cells and myeloma cellsMatching Questions

Question 7

Match between columns
                        Premises: Found in between the CH1 and CH2 regions of the heavy chain
An antigen seen in the binding region of an immunoglobulin molecule
After treating antibody with urea and mercaptoethanol you get this or these
After treating an antibody with papain you get this or these

Accepted Answer

The Answer of idiotype and the hinge region and 2 is...

Question 8

Protein A is a protein

Accepted Answer

A) on the surface of Staphylococcus aureus 
B) which binds immunoglobulin 
C) both of the above 
D) none of the above 
A) on the surface of Staphylococcus aureus 
B) which binds immunoglobulin 
C) both of the above 
D) none of the above

Question 9

The antibody molecule that has 10 binding sites but because of steric hinderance often binds only five antigen molecules is

Accepted Answer

A) IgA 
B) IgM 
C) IgG 
D) IgD 
E) IgE 
A) IgA 
B) IgM 
C) IgG 
D) IgD 
E) IgE

Question 10

Two immunoglobulin classes that have a J chain are

Accepted Answer

A) IgA and IgM 
B) IgM and IgG 
C) IgG and IgD 
D) IgD and IgE 
E) IgE and IgA 
A) IgA and IgM 
B) IgM and IgG 
C) IgG and IgD 
D) IgD and IgE 
E) IgE and IgA

Question 11

Secretory IgA

Accepted Answer

A) is made by one cell 
B) is made by two cells 
C) is made by three cells 
D) none of the above 
A) is made by one cell 
B) is made by two cells 
C) is made by three cells 
D) none of the above

Question 12

The two different cells needed to produce a monoclonal antibody are

Accepted Answer

A) a myeloma cell that survives in the media used after fusion and a spleen cell from an immunized animal 
B) a myeloma cell from an immunized animal and a spleen cell that survives in the media used after fusion 
C) a myeloma cell that does not survive in the media used after fusion and a spleen cell from an immunized animal 
D) a HAT myeloma cell and a spleen cell from an immunized animal 
A) a myeloma cell that survives in the media used after fusion and a spleen cell from an immunized animal 
B) a myeloma cell from an immunized animal and a spleen cell that survives in the media used after fusion 
C) a myeloma cell that does not survive in the media used after fusion and a spleen cell from an immunized animal 
D) a HAT myeloma cell and a spleen cell from an immunized animal

Question 13

Treating IgG with pepsin causes production of

Accepted Answer

A) the hinge region 
B) 2 Fab and an Fc 
C) (Fab')2 +Fc fragments 
D) heavy and light chains 
E) epitopes 
A) the hinge region 
B) 2 Fab and an Fc 
C) (Fab')2 +Fc fragments 
D) heavy and light chains 
E) epitopes

Question 14

The paratope that is seen as an antigen is called an

Accepted Answer

A) allotrope 
B) isotype 
C) idiotype 
D) allotype 
A) allotrope 
B) isotype 
C) idiotype 
D) allotype

Question 15

Denaturing and breaking disulfide bonds gives

Accepted Answer

A) the hinge regions 
B) 2 Fab and an Fc 
C) (Fab')2 +Fc fragments 
D) heavy and light chains 
A) the hinge regions 
B) 2 Fab and an Fc 
C) (Fab')2 +Fc fragments 
D) heavy and light chains

Question 16

Immunoglobulin diversity is produced in three stages. Which stage(s) is(are) accomplished after interaction with antigen?

Accepted Answer

A) random recombinational events of DNA gene segments during B cell maturation 
B) clonal deletion of self-reactive B cells 
C) somatic mutation and affinity maturation 
D) A and B 
E) B and C 
A) random recombinational events of DNA gene segments during B cell maturation 
B) clonal deletion of self-reactive B cells 
C) somatic mutation and affinity maturation 
D) A and B 
E) B and C

Question 17

The antibody that is protective in serum, is made after IgM, and does not cause allergy is

Accepted Answer

A) IgA 
B) IgM 
C) IgG 
D) IgD 
E) IgE 
A) IgA 
B) IgM 
C) IgG 
D) IgD 
E) IgE

Question 18

In the original experiment which determined the structure of immunoglobulin, it was broken into heavy and light chains using

Accepted Answer

A) papain 
B) pepsin 
C) urea and mercaptoethanol 
D) none of the above 
A) papain 
B) pepsin 
C) urea and mercaptoethanol 
D) none of the above

Question 19

In which peak in the figure is the albumin?

Accepted Answer

A) A 
B) B 
C) C 
D) D 
E) E 
A) A 
B) B 
C) C 
D) D 
E) E

Question 20

One of the immunoglobulin molecules found as a dimer in secretions is

Accepted Answer

A) IgA 
B) IgM 
C) IgG 
D) IgD 
E) IgE 
A) IgA 
B) IgM 
C) IgG 
D) IgD 
E) IgE

Exam 2: Antibody

In serum protein electrophoresis, the protein that moves most quickly toward the anode is

If a particular IgG molecule and IgM molecule have the same affinity, which would have a higher avidity?

IgG, IgM, IgA, and IgE

At pH 8.6, most serum proteins

A spleen cell and a myeloma cell are used to produce a monoclonal antibody. How do they help produce a monoclonal antibody?

Monoclonal antibodies are produced by cells that are hybrids of

Protein A is a protein

The antibody molecule that has 10 binding sites but because of steric hinderance often binds only five antigen molecules is

Two immunoglobulin classes that have a J chain are

Secretory IgA

The two different cells needed to produce a monoclonal antibody are

Treating IgG with pepsin causes production of

The paratope that is seen as an antigen is called an

Denaturing and breaking disulfide bonds gives

Immunoglobulin diversity is produced in three stages. Which stage(s) is(are) accomplished after interaction with antigen?

The antibody that is protective in serum, is made after IgM, and does not cause allergy is

In the original experiment which determined the structure of immunoglobulin, it was broken into heavy and light chains using

In which peak in the figure is the albumin?

One of the immunoglobulin molecules found as a dimer in secretions is

Introduction to Immunology

Antigens-Epitopes and Immunogenicity

Cellular Immunity

Complement Pathways

Agglutination and Precipitation Reactions: the Unlabeled Immunoassays

Labeled Immunoassays

Serology Laboratory Math

Hypersensitivity Reactions

Autoimmunitysystemic

Autoimmunityorgan or Tissue Specific

Tumor Immunology

Hematopoeitc Malignancies

Immunology of Transplantation

Primary Immunodeficiency Diseases

Acquired Immunodeficiency Including Hiv and Aids

Hepatitis

Serology and Viruses Herpesviridae Family and Other Serologically

Bacterial Serology

Fungal Serology Parasitic Serology

Forensic Serology

Safety

Molecular Biology

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