Question 1

What are the advantages of using algae for biofuels?

Accepted Answer

Algae are able to uptake CO₂ from the atm

Question 2

How is Saccharomyces cerevisiae used in genome transplantation?

Accepted Answer

A)  for assembly of large fragments by homologous recombination 
B)  for site-directed mutagenesis and correction of assembly errors 
C)  as the final host of the transplanted genome 
D)  as a host for the viruses into which the genome is transplanted 
E)  to produce the recombinant lambda-Red ssDNA-binding protein 
A)  for assembly of large fragments by homologous recombination 
B)  for site-directed mutagenesis and correction of assembly errors 
C)  as the final host of the transplanted genome 
D)  as a host for the viruses into which the genome is transplanted 
E)  to produce the recombinant lambda-Red ssDNA-binding protein

Question 3

Bifurcating three-node transcriptional networks are common motif in E.coli.

Accepted Answer

A) True 
 B)False

Question 4

How is a toggle switch different from a repressilator?

Accepted Answer

A)  A repressilator oscillates whereas a toggle switch is bistable. 
B)  Repressilators encode repressors (e.g.LacI),but toggle switches do not. 
C)  A repressilator requires a control molecule (e.g.IPTG),whereas a toggle switch does not. 
D)  Toggle switches encode repressors (e.g.LacI),but repressilators do not. 
E)  Anhydrotetracycline is an effective regulatory of repressilators,but not of toggle switches. 
A)  A repressilator oscillates whereas a toggle switch is bistable. 
B)  Repressilators encode repressors (e.g.LacI),but toggle switches do not. 
C)  A repressilator requires a control molecule (e.g.IPTG),whereas a toggle switch does not. 
D)  Toggle switches encode repressors (e.g.LacI),but repressilators do not. 
E)  Anhydrotetracycline is an effective regulatory of repressilators,but not of toggle switches.

Question 5

Genome transplantation has successfully converted one species into another.

Accepted Answer

A) True 
 B)False

Question 6

What outcomes of the human genome project serve as a foundation for systems biology?

Accepted Answer

The human genome project (HGP)produced m

Question 7

Polymerase cycling assembly is a process useful in genome synthesis.

Accepted Answer

A) True 
 B)False

Question 8

An emergent property is what happens to a system when it is in a disease state.

Accepted Answer

A) True 
 B)False

Question 9

Building artificial systems is an effective way to test our understanding of natural systems.

Accepted Answer

A) True 
 B)False

Question 10

Which system is most likely to produce the stimulus-response curve shown here?  
In the systems shown below,the concentration of molecule 3 represents the stimulus,and the concentration of molecule 2 is the response.Molecule 1 is a substrate of molecule 3.Molecule 4 is an inactive form of molecule 3.Molecule 2 activates molecule 5.

Accepted Answer

A) 
  
B) 
  
C) 
  
D) 
  
E) 
  
A) 
  
B) 
  
C) 
  
D) 
  
E)

Question 11

Which of the following is not one of the main questions asked by systems biology?

Accepted Answer

A)  How do perturbations affect the system? 
B)  What are the emergent properties? 
C)  What is the molecular function of a specific gene of interest? 
D)  What are the associations among the components? 
E)  What are the elements of the system? 
A)  How do perturbations affect the system? 
B)  What are the emergent properties? 
C)  What is the molecular function of a specific gene of interest? 
D)  What are the associations among the components? 
E)  What are the elements of the system?

Question 12

Systems biology attempts to define all the components of a biological system and understand how they function in conjunction with one another.

Accepted Answer

A) True 
 B)False

Question 13

How can MAGE (Multiplex Automated Genome Engineering)be used to mimic evolution?

Accepted Answer

The use of degenerate pools of ssDNA oli

Question 14

What does it mean for a synthetic circuit to interface with natural cellular processes?

Accepted Answer

A)  Natural genes can be used as a foundation for design of synthetic circuits. 
B)  Complete synthetic genomes are transplanted into natural cells. 
C)  Small fragments of synthetic DNA are incorporated into living cells. 
D)  The output of a synthetic circuit is controlled by an endogenous signal. 
E)  Natural metabolic pathways can beengineered by site-directed mutagenesis. 
A)  Natural genes can be used as a foundation for design of synthetic circuits. 
B)  Complete synthetic genomes are transplanted into natural cells. 
C)  Small fragments of synthetic DNA are incorporated into living cells. 
D)  The output of a synthetic circuit is controlled by an endogenous signal. 
E)  Natural metabolic pathways can beengineered by site-directed mutagenesis.

Question 15

Transcription factors can regulate the expression of other transcription factors.

Accepted Answer

A) True 
 B)False

Question 16

Given the functional genetic toggle switch shown,which of the following would result in GFP expression?

Accepted Answer

A)  treatment with IPTG 
B)  treatment with aTc 
C)  treatment with aTc followed by removal of aTc from the system 
D)  deletion of the Pro-2 promoter 
E)  deletion of the Pro-2 promoter and deletion of the Pro-1 promoter 
A)  treatment with IPTG 
B)  treatment with aTc 
C)  treatment with aTc followed by removal of aTc from the system 
D)  deletion of the Pro-2 promoter 
E)  deletion of the Pro-2 promoter and deletion of the Pro-1 promoter

Question 17

The leader of the team that produced artemisinin in yeast is J.Craig Venter.

Accepted Answer

A) True 
 B)False

Question 18

The leader of the team that created the first synthetic genome is J.Craig Venter.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following is an example of an emergent property?

Accepted Answer

A)  Functioning of a group of molecules as a bistable switch. 
B)  Identification of a novel mutation in a developmental pathway. 
C)  Binding of allolactose to the lac repressor. 
D)  Reverse transcription of a viral coat protein gene. 
E)  Binding of allolactose to the lac operator. 
A)  Functioning of a group of molecules as a bistable switch. 
B)  Identification of a novel mutation in a developmental pathway. 
C)  Binding of allolactose to the lac repressor. 
D)  Reverse transcription of a viral coat protein gene. 
E)  Binding of allolactose to the lac operator.

Question 20

Discovery science is used to identify all the elements of a biological system rather than to test a specific hypothesis.

Accepted Answer

A) True 
 B)False

Exam 22: Exploring Synthetic Biology: Concepts, Techniques, and Applications

What are the advantages of using algae for biofuels?

How is Saccharomyces cerevisiae used in genome transplantation?

Bifurcating three-node transcriptional networks are common motif in E.coli.

How is a toggle switch different from a repressilator?

Genome transplantation has successfully converted one species into another.

What outcomes of the human genome project serve as a foundation for systems biology?

Polymerase cycling assembly is a process useful in genome synthesis.

An emergent property is what happens to a system when it is in a disease state.

Building artificial systems is an effective way to test our understanding of natural systems.

Which of the following is not one of the main questions asked by systems biology?

Systems biology attempts to define all the components of a biological system and understand how they function in conjunction with one another.

How can MAGE (Multiplex Automated Genome Engineering)be used to mimic evolution?

What does it mean for a synthetic circuit to interface with natural cellular processes?

Transcription factors can regulate the expression of other transcription factors.

Given the functional genetic toggle switch shown,which of the following would result in GFP expression?

The leader of the team that produced artemisinin in yeast is J.Craig Venter.

The leader of the team that created the first synthetic genome is J.Craig Venter.

Which of the following is an example of an emergent property?

Discovery science is used to identify all the elements of a biological system rather than to test a specific hypothesis.

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

Dna Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Genomes

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

Manipulating the Genomes of Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Variation and Selection in Populations

Genetics of Complex Traits

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