Exam 17: Manipulating the Genomes of Eukaryotes

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Mitochondria and chloroplasts are considered to be semiautonomous.

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Mutations in mtDNA are often caused by free-radical damage,which results from oxidative phosphorylation.

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Is the so-called "universal" genetic code truly universal? Why or why not?

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A bacterium is found that is resistant to the antibiotic gentamicin.The bacterium was isolated in a hospital where patients were routinely given gentamicin for a variety of infections.What was the pressure that selected for this resistant population?

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Small circular dsDNA molecules carrying non-essential genes,called plasmids,may exist as multiple copies in some bacteria.

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We isolate nine different mutants unable to make histidine.They map in different locations and by genetic means we can show there are nine different genes in the pathway.How many enzymes can we anticipate finding in this pathway?

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Explain the difference between the F-,F+,Hfr,and F designations.

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Certain protozoa engulf cyanobacteria (photosynthetic bacteria)and maintain a given number of the cyanobacteria even when they divide.The protozoa divide only after the cyanobacteria have doubled their number.The cyanobacteria excrete glucose that the protozoa use for energy.This is a good example of

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Since a mutation in an essential gene prevents a colony from growing,how would a bacteriologist study such a strain?

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Mother 1 and Mother 2 both show a hypothetical mitochondria-related phenotype,and neither father is affected.Both families have four children.The children of family 1 are all affected,but only half of the children of family 2 show the phenotype.Interpret this result with respect to the genotype of Mother 1 and Mother 2.

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The universal code of DNA is the same for mtDNA as for chromosomal DNA.

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A homoplasmic cell carries

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Which technique is used to make a copy of the colonies on a petri plate? One plate can be analyzed by a destructive method,while the other is retained for future use.

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Rhizobia are bacteria that live symbiotically in the roots of leguminous plants.It has been proposed that some enzymes in the bacteria were derived from the plant.What mechanism would most likely have been involved in this?

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Artificial transformation methods must be used on most bacteria to make them competent.

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With traits showing maternal inheritance,such as leaf variegation in four o'clocks,reciprocal crosses usually

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A researcher hopes that a plant cell,after being shot with a gene gun,will

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Heteroplasmic cells lead to earlier appearance of mitochondrially inherited disease compared to homoplasmic cells.

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Which of the following statements is correct with respect to mitochondrial genes?

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An Hfr strain has a plasmid inserted near the trp (tryptophan)locus.This Hfr strain is incubated with an F- trp- strain.We isolate a trp+ strain that can readily donate this trait to other trp- strains.No other traits are transferred.What has happened?

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