Question 1

We spread 109 bacteria on a plate with 25 µg streptomycin/mL media.Four colonies grow.These colonies contain bacteria that are

Accepted Answer

A)  wild-type. 
B)  resistant to streptomycin. 
C)  resistant to all antibiotics. 
D)  able to grow in unusual circumstance. 
E)  infected with bacteriophage. 
A)  wild-type. 
B)  resistant to streptomycin. 
C)  resistant to all antibiotics. 
D)  able to grow in unusual circumstance. 
E)  infected with bacteriophage.

Question 2

Semiautonomous as applied to mitochondria and chloroplasts means that they require gene products encoded in the cell's nuclear DNA.

Accepted Answer

A) True 
 B)False

Question 3

When eukaryotic cells are stained with DNA-specific dyes and viewed under the light microscope,DNA molecules are revealed in

Accepted Answer

A)  the nucleus only. 
B)  the mitochondrion only 
C)  the chloroplasts only. 
D)  all these places: nucleus,mitochondrion,and chloroplast. 
E)  the mitochondrion and chloroplast only. 
A)  the nucleus only. 
B)  the mitochondrion only 
C)  the chloroplasts only. 
D)  all these places: nucleus,mitochondrion,and chloroplast. 
E)  the mitochondrion and chloroplast only.

Question 4

Explain why different Hfr cells can have F plasmids integrated at different sites.

Accepted Answer

Hfr cells are created when F plasmids in

Question 5

Inhibitors of bacterial translation,such as chloramphenicol and erythromycin,generally

Accepted Answer

A)  are inhibitors of eukaryotic cytoplasmic protein synthesis. 
B)  are inhibitors of mitochondrial protein synthesis. 
C)  have no effect on mitochondrial protein synthesis. 
D)  are inhibitors of eukaryotic cytoplasmic protein synthesis and have no effect on mitochondrial protein synthesis. 
E)  are inhibitors of microtubules and cell division. 
A)  are inhibitors of eukaryotic cytoplasmic protein synthesis. 
B)  are inhibitors of mitochondrial protein synthesis. 
C)  have no effect on mitochondrial protein synthesis. 
D)  are inhibitors of eukaryotic cytoplasmic protein synthesis and have no effect on mitochondrial protein synthesis. 
E)  are inhibitors of microtubules and cell division.

Question 6

Describe the difference between "selection" and "screen" with respect to mutant isolation methods.

Accepted Answer

A "selection" uses conditions in which o

Question 7

Metagenomics is the analysis of genomic DNA from a community or habitat.Why would such studies be useful?

Accepted Answer

Because so few bacterial species can be

Question 8

Mitochondria in brain cells of patients with symptoms of Alzheimer's disease have unusually high energy metabolism.

Accepted Answer

A) True 
 B)False

Question 9

The proportion of mutant mtDNAs and the tissue in which they reside influence phenotype.

Accepted Answer

A) True 
 B)False

Question 10

Which of the following relationships can be proven using mtDNA?

Accepted Answer

A)  Boy and his mother's brother 
B)  Boy and his father's sister 
C)  Girl and her mother's father 
D)  Girl and her father's mother 
E)  Girl and her father's sister 
A)  Boy and his mother's brother 
B)  Boy and his father's sister 
C)  Girl and her mother's father 
D)  Girl and her father's mother 
E)  Girl and her father's sister

Question 11

We grow resistant (Strr)cells to a concentration of 109 and plate them on a medium with 25 µg/mL gentamicin.Six colonies grow.These cells should be

Accepted Answer

A)  Strr. 
B)  Genr. 
C)  Strr Genr. 
D)  wild-type. 
E)  Strr Gens. 
A)  Strr. 
B)  Genr. 
C)  Strr Genr. 
D)  wild-type. 
E)  Strr Gens.

Question 12

Identical twins arise from a single zygote.One twin has symptoms of MERFF but the other twin does not show the disorder.Explain how this might happen.

Accepted Answer

Unequal distribution of mitochondria dur

Question 13

RNA editing can occur in mitochondrial transcripts and is the addition and/or deletion of uracils and/or cytosines,which results in codon changes from the original DNA.

Accepted Answer

A) True 
 B)False

Question 14

An integrated copy of a temperate bacteriophage is called a prophage.

Accepted Answer

A) True 
 B)False

Question 15

Describe the difference between generalized and specialized transduction.

Accepted Answer

Transduction is a form of gene transfer

Question 16

In uniparental inheritance,transmission is usually maternal.

Accepted Answer

A) True 
 B)False

Question 17

Which of the follow is not true of a bacterial chromosome?

Accepted Answer

A)  is circular. 
B)  is covalently closed. 
C)  is double-stranded DNA. 
D)  contains mobile sequences. 
E)  is a left-handed helix. 
A)  is circular. 
B)  is covalently closed. 
C)  is double-stranded DNA. 
D)  contains mobile sequences. 
E)  is a left-handed helix.

Question 18

Which of the following is NOT accurate with regard to organelle genomes?

Accepted Answer

A)  mtDNA and cpDNA are usually organized into nucleosomes by histones. 
B)  Mitochondrial translation is often inhibited by bacterial antibiotics (e.g. ,chloramphenicol). 
C)  Like bacteria,mitochondria use N-formyl methionine and tRNAfMet in translation. 
D)  Organelle chromosomes are typically circular. 
E)  Organelle genomes are usually present in multiple copies. 
A)  mtDNA and cpDNA are usually organized into nucleosomes by histones. 
B)  Mitochondrial translation is often inhibited by bacterial antibiotics (e.g. ,chloramphenicol). 
C)  Like bacteria,mitochondria use N-formyl methionine and tRNAfMet in translation. 
D)  Organelle chromosomes are typically circular. 
E)  Organelle genomes are usually present in multiple copies.

Question 19

Oxidative phosphorylation decreases with age.

Accepted Answer

A) True 
 B)False

Question 20

Plasmids are small,circular pieces of DNA that some bacteria carry in addition to their own chromosome.Plasmids may include genes that benefit the bacterial host under certain conditions.Describe what some of these genes may be.

Accepted Answer

One important group of plasmids (the F p

Exam 17: Manipulating the Genomes of Eukaryotes

We spread 109 bacteria on a plate with 25 µg streptomycin/mL media.Four colonies grow.These colonies contain bacteria that are

Semiautonomous as applied to mitochondria and chloroplasts means that they require gene products encoded in the cell's nuclear DNA.

When eukaryotic cells are stained with DNA-specific dyes and viewed under the light microscope,DNA molecules are revealed in

Explain why different Hfr cells can have F plasmids integrated at different sites.

Inhibitors of bacterial translation,such as chloramphenicol and erythromycin,generally

Describe the difference between "selection" and "screen" with respect to mutant isolation methods.

Metagenomics is the analysis of genomic DNA from a community or habitat.Why would such studies be useful?

Mitochondria in brain cells of patients with symptoms of Alzheimer's disease have unusually high energy metabolism.

The proportion of mutant mtDNAs and the tissue in which they reside influence phenotype.

Which of the following relationships can be proven using mtDNA?

We grow resistant (Strr)cells to a concentration of 109 and plate them on a medium with 25 µg/mL gentamicin.Six colonies grow.These cells should be

Identical twins arise from a single zygote.One twin has symptoms of MERFF but the other twin does not show the disorder.Explain how this might happen.

RNA editing can occur in mitochondrial transcripts and is the addition and/or deletion of uracils and/or cytosines,which results in codon changes from the original DNA.

An integrated copy of a temperate bacteriophage is called a prophage.

Describe the difference between generalized and specialized transduction.

In uniparental inheritance,transmission is usually maternal.

Which of the follow is not true of a bacterial chromosome?

Which of the following is NOT accurate with regard to organelle genomes?

Oxidative phosphorylation decreases with age.

Plasmids are small,circular pieces of DNA that some bacteria carry in addition to their own chromosome.Plasmids may include genes that benefit the bacterial host under certain conditions.Describe what some of these genes may be.

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

Dna Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Genomes

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Variation and Selection in Populations

Genetics of Complex Traits

Exploring Synthetic Biology: Concepts, Techniques, and Applications

Filters

We spread 10⁹ bacteria on a plate with 25 µg streptomycin/mL media.Four colonies grow.These colonies contain bacteria that are

We grow resistant (Str^r)cells to a concentration of 10⁹ and plate them on a medium with 25 µg/mL gentamicin.Six colonies grow.These cells should be