Exam 17: Manipulating the Genomes of Eukaryotes

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Variegated four o'clock leaves have white patches among the green areas due to

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Explain briefly the genetic technique known as recombineering.

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Specialized transduction allows phages to pick up only adjacent DNA from the host chromosome during the transition from the lysogenic to the lytic cycle.

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cpDNA-encoded proteins include

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Explain how mitochondrial traits are inherited.

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The molecules used in a chloroplast to carry out photosynthesis:

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What would happen if a transposon were to "jump" into the origin of the bacteria genome?

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The codon UGA specifies

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An open reading frame is a long stretch of DNA that codes for amino acids and is uninterrupted by a stop codon.

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Why might a researcher attach a gene encoding a green fluorescent protein to a DNA sequence before transforming a cell's mtDNA?

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Electroporation is the use of high voltage and high calcium salt to force foreign DNA into bacteria.

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RNA editing occurs in the mitochondria of most organisms.

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Human mitochondrial DNA contains tightly packed genes without introns.

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________ are small circular pieces of DNA frequently found in bacteria.

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Describe how you would screen for Arg- cells arising from a wild-type culture of E.coli. To increase frequency of mutation,first expose wild-type culture to a mutagen.Then plate mutagenized cells onto minimal plates containing arginine.After colonies have grown up,replica plate onto minimal plates without arginine.Note position of cells that grow on first set of plates but not the second set.Those are the Arg- mutants.

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A transductant is a bacterium that has been the recipient of donor DNA from a bacteriophage.

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Which of the following has been associated with Alzheimer's disease?

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