Exam 22: Gene Expression: II Protein Synthesis and Sorting

The appropriate folding of a newly translated protein is essential, and chronic misfolding often leads to disease, especially in vertebrates. This misfolding, particularly in the case of prion diseases, may be due to a defect in

In studying an E. coli strain containing a known frameshift mutation, you isolate a mutant in which this frameshift is suppressed. You therefore expect to find which of the following associated with the suppression? (Assume the frameshift has not been corrected in the coding for the peptide.)

Using the techniques of genetic engineering, you design a cytoplasmic protein you want to accumulate within the ER of yeast cells. To accomplish this goal, you need to

Ribosomes are very old structures, found in all organisms (except viruses)from bacteria to humans. As a result, molecular taxonomists are looking at relationships based upon which rRNA sequence(s)?

Which of the following activities is NOT associated with post- translational processing?

Release factors of translation recognize the codon

Which of the following requires eIFs for initiation?

Which of the following steps in prokaryotic translation takes place first?

In the following gene sequence, the original sequence is ABC* DEFGH. It now reads ABC* DGFEH. The * indicates the centromere. This type of mutation is a (an)

Once an amino acid has been attached to the appropriate tRNA, the tRNA is called a(n) and the amino acid is said to be .

If more than one codon specifies the same amino acid, the code is said to be

Messenger RNAs containing no stop codons due to a frameshift mutation are eliminated by

The enzymes link amino acids with their appropriate tRNAs.

Ribosomes that do not anchor on the surface of the rough endoplasmic reticulum during translation MOST probably

Of the following steps associated with translation, which does NOT involve hydrolysis of GTP?

Which of the following is NOT a prion disease?

In cloning a gene, you discover that the gene product is much longer than should be observed based upon the insert you successfully cloned. What problems would result in a longer cloned sequence? How will you go about investigating your hypothesis? Would your answer change based upon the presence or absence of introns? Why or why not?

A mutant bacterium is isolated. This bacterial strain has the characteristic of very slow, "sickly" growth. You isolate mRNA from this bacteria to use in an in vitro translation system and note that even in vitro it is difficult to use this message and obtain protein. Of the following, which is a likely explanation for these results?