Question 1

To what are the reflection positions and intensities on the photographic plate of an X-ray crystallography experiment related?

Accepted Answer

A) proton densities of proteins 
B) neutron densities of proteins 
C) electron densities of proteins 
D) proton densities of nucleic acids 
E) neutron densities of lipids 
A) proton densities of proteins 
B) neutron densities of proteins 
C) electron densities of proteins 
D) proton densities of nucleic acids 
E) neutron densities of lipids

Question 2

If you are illuminating a specimen with ultraviolet light, into what kind of container should it be placed to make a measurement in a spectrophotometer?

Accepted Answer

A) in a basinette 
B) in a special, flat-sided quartz container 
C) in a borosilicate glass tube 
D) in a special, flat-sided glass container 
E) in a special, flat-sided plastic container 
A) in a basinette 
B) in a special, flat-sided quartz container 
C) in a borosilicate glass tube 
D) in a special, flat-sided glass container 
E) in a special, flat-sided plastic container

Question 3

Why have most clinical trials in which retroviruses have been used to introduce genes for the purpose of gene therapy in patients lacking that gene been unsuccessful?

Accepted Answer

A) Viral DNA is more unstable than mammalian DNA. 
B) Current viral vectors have too high an infection efficiency rate. 
C) Current viral vectors have too low an infection efficiency rate. 
D) Mammalian DNA is more unstable than viral DNA. 
E) Current viral vectors tend to cause serious diseases. 
A) Viral DNA is more unstable than mammalian DNA. 
B) Current viral vectors have too high an infection efficiency rate. 
C) Current viral vectors have too low an infection efficiency rate. 
D) Mammalian DNA is more unstable than viral DNA. 
E) Current viral vectors tend to cause serious diseases.

Question 4

What step must first be carried out before RNA templates can be amplified?

Accepted Answer

A) Denaturation must be carried out first. 
B) They must first be converted to complementary DNAs using DNA polymerase. 
C) They must first be converted to complementary RNAs using RNA polymerase. 
D) They must first be converted to complementary DNAs using reverse transcriptase. 
E) They must first be converted to complementary DNAs using Taq polymerase. 
A) Denaturation must be carried out first. 
B) They must first be converted to complementary DNAs using DNA polymerase. 
C) They must first be converted to complementary RNAs using RNA polymerase. 
D) They must first be converted to complementary DNAs using reverse transcriptase. 
E) They must first be converted to complementary DNAs using Taq polymerase.

Question 5

Which enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

Accepted Answer

A) RNase H 
B) reverse transcriptase 
C) DNA polymerase I 
D) RNase K 
E) DNA ligase 
A) RNase H 
B) reverse transcriptase 
C) DNA polymerase I 
D) RNase K 
E) DNA ligase

Question 6

What is NOT an advantage of capturing the image generated by transmission electron microscopy using a CCD video camera?

Accepted Answer

A) It provides an instant image. 
B) There is no need to develop the image using chemicals. 
C) The image can be captured at very high resolution. 
D) Less voltage is required. 
A) It provides an instant image. 
B) There is no need to develop the image using chemicals. 
C) The image can be captured at very high resolution. 
D) Less voltage is required.

Question 7

What method is typically used to dry specimens for scanning electron microscopy?

Accepted Answer

A) air drying 
B) critical-point drying 
C) forced air drying 
D) vacuum drying 
E) dehydrational drying 
A) air drying 
B) critical-point drying 
C) forced air drying 
D) vacuum drying 
E) dehydrational drying

Question 8

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

Accepted Answer

A) if it has a greater density than the surrounding medium 
B) if it has a lower density than the surrounding medium 
C) if it is wet 
D) if the surrounding medium is denser than the component 
E) if the component and the surrounding medium have equal density 
A) if it has a greater density than the surrounding medium 
B) if it has a lower density than the surrounding medium 
C) if it is wet 
D) if the surrounding medium is denser than the component 
E) if the component and the surrounding medium have equal density

Question 9

Why is it that with a standard transmission electron microscope, resolution is actually about two orders of magnitude less than its theoretical limit?

Accepted Answer

A) The lenses of an electron microscope exhibit serious spherical aberration problems, which can be avoided by using a very small N. A. 
B) The lenses of an electron microscope exhibit serious spherical aberration problems, which can be avoided by using a very large N. A. 
C) If electron wavelength is too high, spherical aberration distorts the image. 
D) If electron wavelength is too low, spherical aberration distorts the image. 
A) The lenses of an electron microscope exhibit serious spherical aberration problems, which can be avoided by using a very small N. A. 
B) The lenses of an electron microscope exhibit serious spherical aberration problems, which can be avoided by using a very large N. A. 
C) If electron wavelength is too high, spherical aberration distorts the image. 
D) If electron wavelength is too low, spherical aberration distorts the image.

Question 10

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

Accepted Answer

A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob 
A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob

Question 11

A collection of cloned DNA fragments is called a _________.

Accepted Answer

A) DNA collection 
B) DNA file 
C) DNA library 
D) DNA biblion 
E) DNA codon 
A) DNA collection 
B) DNA file 
C) DNA library 
D) DNA biblion 
E) DNA codon

Question 12

What size, on average. are the DNA fragments that can be placed into phage λ and cloned?

Accepted Answer

A) 15 kb in length 
B) 20 kb in length 
C) 50 kb in length 
D) 100 kb in length 
E) 10 kb in length 
A) 15 kb in length 
B) 20 kb in length 
C) 50 kb in length 
D) 100 kb in length 
E) 10 kb in length

To what are the reflection positions and intensities on the photographic plate of an X-ray crystallography experiment related?

If you are illuminating a specimen with ultraviolet light, into what kind of container should it be placed to make a measurement in a spectrophotometer?

Why have most clinical trials in which retroviruses have been used to introduce genes for the purpose of gene therapy in patients lacking that gene been unsuccessful?

What step must first be carried out before RNA templates can be amplified?

Which enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

What is NOT an advantage of capturing the image generated by transmission electron microscopy using a CCD video camera?

What method is typically used to dry specimens for scanning electron microscopy?

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

Why is it that with a standard transmission electron microscope, resolution is actually about two orders of magnitude less than its theoretical limit?

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

A collection of cloned DNA fragments is called a _________.

What size, on average. are the DNA fragments that can be placed into phage λ and cloned?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Exam 18: Techniques in Cell and Molecular Biology

To what are the reflection positions and intensities on the photographic plate of an X-ray crystallography experiment related?

If you are illuminating a specimen with ultraviolet light, into what kind of container should it be placed to make a measurement in a spectrophotometer?

Why have most clinical trials in which retroviruses have been used to introduce genes for the purpose of gene therapy in patients lacking that gene been unsuccessful?

What step must first be carried out before RNA templates can be amplified?

Which enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

What is NOT an advantage of capturing the image generated by transmission electron microscopy using a CCD video camera?

What method is typically used to dry specimens for scanning electron microscopy?

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

Why is it that with a standard transmission electron microscope, resolution is actually about two orders of magnitude less than its theoretical limit?

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

A collection of cloned DNA fragments is called a _________.

What size, on average. are the DNA fragments that can be placed into phage λ and cloned?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters