Question 1

How are fragments already in a mass spectrometer further fragmented?

Accepted Answer

A) injection of pepsin 
B) injection of more trypsin 
C) collision of peptides with an inert gas 
D) collision of peptides with nitrogen gas 
E) collision of peptides with oxygen 
A) injection of pepsin 
B) injection of more trypsin 
C) collision of peptides with an inert gas 
D) collision of peptides with nitrogen gas 
E) collision of peptides with oxygen

Question 2

When a cell is growing on a culture dish, what is the word that refers to the lower surface of the cell facing toward the substratum?

Accepted Answer

A) anterior 
B) dorsal 
C) posterior 
D) ventral 
E) caudal 
A) anterior 
B) dorsal 
C) posterior 
D) ventral 
E) caudal

Question 3

A culture system in which cells are grown in a multi-dimensional matrix consisting of synthetic and/or natural extracellular materials is called a ______.

Accepted Answer

A) three-dimensional culture system 
B) two-dimensional culture system 
C) matrix culture system 
D) secondary culture system 
E) organic culture system 
A) three-dimensional culture system 
B) two-dimensional culture system 
C) matrix culture system 
D) secondary culture system 
E) organic culture system

Question 4

If the template in the cycle sequencing method is a PCR product, what is generally used as a primer?

Accepted Answer

A) both PCR primers 
B) randomly generated primers 
C) only one of the PCR primers 
D) any primer except the PCR primers 
E) poly(
A) oligonucleotides 
A) both PCR primers 
B) randomly generated primers 
C) only one of the PCR primers 
D) any primer except the PCR primers 
E) poly(
A) oligonucleotides

Question 5

If you do site-directed mutagenesis of the coding region of a gene, which of the following is most likely to happen?

Accepted Answer

A) The rate of gene expression slows down. 
B) The rate of gene expression speeds up. 
C) The protein's structure and function are altered. 
D) The protein's structure and function remain unaltered. 
A) The rate of gene expression slows down. 
B) The rate of gene expression speeds up. 
C) The protein's structure and function are altered. 
D) The protein's structure and function remain unaltered.

Question 6

How can a researcher visualize the DNA fragments separated in an electrophoresis gel?

Accepted Answer

A) using a labeled probe with a sequence complementary to the desired DNA fragment 
B) staining with ethidium bromide 
C) staining with potassium chloride 
D) using labeled antibodies 
E) staining with Coomassie Blue 
A) using a labeled probe with a sequence complementary to the desired DNA fragment 
B) staining with ethidium bromide 
C) staining with potassium chloride 
D) using labeled antibodies 
E) staining with Coomassie Blue

Question 7

What is the term used to describe a piece of DNA containing sequences derived from more than one organism?

Accepted Answer

A) combinational DNA 
B) recombinant DNA 
C) combined DNA 
D) cloned DNA 
E) recreational DNA 
A) combinational DNA 
B) recombinant DNA 
C) combined DNA 
D) cloned DNA 
E) recreational DNA

Question 8

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA. The ends of the altered DNA are then ligated back together. What is the effect on the gene sequence?

Accepted Answer

A) Its amino acids remain constant 
B) The reading frame of the gene is destroyed. 
C) The gene is unchanged. 
D) The gene is transposed. 
E) The gene is inverted. 
A) Its amino acids remain constant 
B) The reading frame of the gene is destroyed. 
C) The gene is unchanged. 
D) The gene is transposed. 
E) The gene is inverted.

Question 9

What is the name of the special device used to section a frozen block of tissue?

Accepted Answer

A) micrometer 
B) cryoslicer 
C) cryomicrotome 
D) ultratome 
E) microslicer 
A) micrometer 
B) cryoslicer 
C) cryomicrotome 
D) ultratome 
E) microslicer

Question 10

Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _________; individual B cell clones which respond to the same antigen have membrane-bound antibodies with an affinity for __________.

Accepted Answer

A) identical antigen-combining sites, different parts of the antigen 
B) variable antigen-combining sites, the same part of the antigen 
C) identical antigen-combining sites, the same part of the antigen 
D) variable antigen-combining sites, different parts of the antigen 
E) different antigen-binding sites, the cell membrane 
A) identical antigen-combining sites, different parts of the antigen 
B) variable antigen-combining sites, the same part of the antigen 
C) identical antigen-combining sites, the same part of the antigen 
D) variable antigen-combining sites, different parts of the antigen 
E) different antigen-binding sites, the cell membrane

Question 11

Advanced optical techniques such as stochastic optical reconstruction microscopy (STORM)have lowered the optical microscope resolution limit to less than ________.

Accepted Answer

A) 0.02 µm 
B) 0.2 µm 
C) 2 µm 
D) 20 µm 
E) 200 µm 
A) 0.02 µm 
B) 0.2 µm 
C) 2 µm 
D) 20 µm 
E) 200 µm

Question 12

In the yeast two-hybrid assay, the _________ domain and the _________ domain of a transcription factor are fused to two different proteins. If the proteins interact with each other, the transcription factor can allow reporter gene expression.

Accepted Answer

A) DNA binding, activation 
B) DNA binding, inhibition 
C) regulation, activation 
D) fish, bait 
E) upper, lower 
A) DNA binding, activation 
B) DNA binding, inhibition 
C) regulation, activation 
D) fish, bait 
E) upper, lower

Question 13

What is the name of the procedure in which single-stranded DNA molecules are separated by electrophoresis, immobilized on a nitrocellulose filter and then hybridized with labeled, single-stranded DNA probes?

Accepted Answer

A) Southern blot 
B) Northern blot 
C) Western blot 
D) Southwestern blot 
E) Eastern blot 
A) Southern blot 
B) Northern blot 
C) Western blot 
D) Southwestern blot 
E) Eastern blot

Question 14

How do enzymes like trypsin release cells from tissues to create preparations of single cells?

Accepted Answer

A) The enzymes digest extracellular domains of proteins that mediate cell adhesion. 
B) The enzymes digest intracellular domains of proteins that mediate cell adhesion. 
C) The enzymes digest intracellular domains of proteins that mediate cytoskeletal function. 
D) The enzymes digest extracellular domains of proteins that mediate cytoskeletal function. 
E) The enzymes digest extracellular domains of proteins that mediate endocytosis. 
A) The enzymes digest extracellular domains of proteins that mediate cell adhesion. 
B) The enzymes digest intracellular domains of proteins that mediate cell adhesion. 
C) The enzymes digest intracellular domains of proteins that mediate cytoskeletal function. 
D) The enzymes digest extracellular domains of proteins that mediate cytoskeletal function. 
E) The enzymes digest extracellular domains of proteins that mediate endocytosis.

Question 15

Microinjection is a technique in which ____________.

Accepted Answer

A) mRNAs are injected directly into the cell nucleus. 
B) DNA is injected directly into the cell nucleus. 
C) DNA is injected directly into the mitochondria. 
D) DNA is injected directly into the cell cytoplasm. 
E) DNA is injected directly into phagosomes. 
A) mRNAs are injected directly into the cell nucleus. 
B) DNA is injected directly into the cell nucleus. 
C) DNA is injected directly into the mitochondria. 
D) DNA is injected directly into the cell cytoplasm. 
E) DNA is injected directly into phagosomes.

Question 16

Two fluorescent proteins (protein 1 and protein 2)are used in a FRET experiment and are illuminated with light that excites protein 1. If the proteins are close enough for energy transfer to occur, what wavelength(s)of light will be detected?

Accepted Answer

A) The light that excites protein 1 
B) The light that is emitted by protein 1 
C) The light that excites protein 2 
D) The light that is emitted by protein 2 
E) No light will be detected, because the fluorescence of proteins 1 and 2 will cancel out 
A) The light that excites protein 1 
B) The light that is emitted by protein 1 
C) The light that excites protein 2 
D) The light that is emitted by protein 2 
E) No light will be detected, because the fluorescence of proteins 1 and 2 will cancel out

Question 17

An antibody preparation made by a single colony or clone of antibody-producing cells would consist of large quantities of antibodies that all had exactly the same antigen-combining site. Such antibodies would be called _________ antibodies.

Accepted Answer

A) polyclonal 
B) monoclonal 
C) polyvalent 
D) monotreme 
E) monocular 
A) polyclonal 
B) monoclonal 
C) polyvalent 
D) monotreme 
E) monocular

Question 18

For each protein, there is a pH at which negative and positive charges are equal. This pH is referred to as the ___________.

Accepted Answer

A) pH point 
B) neutralization point 
C) isometric point 
D) isoelectric point 
E) isotonic point 
A) pH point 
B) neutralization point 
C) isometric point 
D) isoelectric point 
E) isotonic point

Question 19

Why does the S value alone not provide a measure of molecular mass?

Accepted Answer

A) It is too difficult to measure. 
B) The speed with which a particle travels through a liquid column is not uniform. 
C) The velocity at which a particle moves through a liquid column depends on a number of factors, including shape. 
D) The velocity of movement accelerates as the particle approaches the bottom of the tube. 
E) The velocity of movement slows up as the particle approaches the top of the tube. 
A) It is too difficult to measure. 
B) The speed with which a particle travels through a liquid column is not uniform. 
C) The velocity at which a particle moves through a liquid column depends on a number of factors, including shape. 
D) The velocity of movement accelerates as the particle approaches the bottom of the tube. 
E) The velocity of movement slows up as the particle approaches the top of the tube.

Question 20

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

Accepted Answer

A) blastoderm 
B) fetus 
C) blastocyst 
D) trophectoderm 
E) inner cell mass 
A) blastoderm 
B) fetus 
C) blastocyst 
D) trophectoderm 
E) inner cell mass

How are fragments already in a mass spectrometer further fragmented?

When a cell is growing on a culture dish, what is the word that refers to the lower surface of the cell facing toward the substratum?

A culture system in which cells are grown in a multi-dimensional matrix consisting of synthetic and/or natural extracellular materials is called a ______.

If the template in the cycle sequencing method is a PCR product, what is generally used as a primer?

If you do site-directed mutagenesis of the coding region of a gene, which of the following is most likely to happen?

How can a researcher visualize the DNA fragments separated in an electrophoresis gel?

What is the term used to describe a piece of DNA containing sequences derived from more than one organism?

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA. The ends of the altered DNA are then ligated back together. What is the effect on the gene sequence?

What is the name of the special device used to section a frozen block of tissue?

Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _; individual B cell clones which respond to the same antigen have membrane-bound antibodies with an affinity for __.

Advanced optical techniques such as stochastic optical reconstruction microscopy (STORM)have lowered the optical microscope resolution limit to less than ________.

In the yeast two-hybrid assay, the _ domain and the _ domain of a transcription factor are fused to two different proteins. If the proteins interact with each other, the transcription factor can allow reporter gene expression.

What is the name of the procedure in which single-stranded DNA molecules are separated by electrophoresis, immobilized on a nitrocellulose filter and then hybridized with labeled, single-stranded DNA probes?

How do enzymes like trypsin release cells from tissues to create preparations of single cells?

Microinjection is a technique in which ____________.

Two fluorescent proteins (protein 1 and protein 2)are used in a FRET experiment and are illuminated with light that excites protein 1. If the proteins are close enough for energy transfer to occur, what wavelength(s)of light will be detected?

An antibody preparation made by a single colony or clone of antibody-producing cells would consist of large quantities of antibodies that all had exactly the same antigen-combining site. Such antibodies would be called _________ antibodies.

For each protein, there is a pH at which negative and positive charges are equal. This pH is referred to as the ___________.

Why does the S value alone not provide a measure of molecular mass?

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Exam 18: Techniques in Cell and Molecular Biology

How are fragments already in a mass spectrometer further fragmented?

When a cell is growing on a culture dish, what is the word that refers to the lower surface of the cell facing toward the substratum?

A culture system in which cells are grown in a multi-dimensional matrix consisting of synthetic and/or natural extracellular materials is called a ______.

If the template in the cycle sequencing method is a PCR product, what is generally used as a primer?

If you do site-directed mutagenesis of the coding region of a gene, which of the following is most likely to happen?

How can a researcher visualize the DNA fragments separated in an electrophoresis gel?

What is the term used to describe a piece of DNA containing sequences derived from more than one organism?

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA. The ends of the altered DNA are then ligated back together. What is the effect on the gene sequence?

What is the name of the special device used to section a frozen block of tissue?

Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _________; individual B cell clones which respond to the same antigen have membrane-bound antibodies with an affinity for __________.

Advanced optical techniques such as stochastic optical reconstruction microscopy (STORM)have lowered the optical microscope resolution limit to less than ________.

In the yeast two-hybrid assay, the _________ domain and the _________ domain of a transcription factor are fused to two different proteins. If the proteins interact with each other, the transcription factor can allow reporter gene expression.

What is the name of the procedure in which single-stranded DNA molecules are separated by electrophoresis, immobilized on a nitrocellulose filter and then hybridized with labeled, single-stranded DNA probes?

How do enzymes like trypsin release cells from tissues to create preparations of single cells?

Microinjection is a technique in which ____________.

Two fluorescent proteins (protein 1 and protein 2)are used in a FRET experiment and are illuminated with light that excites protein 1. If the proteins are close enough for energy transfer to occur, what wavelength(s)of light will be detected?

An antibody preparation made by a single colony or clone of antibody-producing cells would consist of large quantities of antibodies that all had exactly the same antigen-combining site. Such antibodies would be called _________ antibodies.

For each protein, there is a pH at which negative and positive charges are equal. This pH is referred to as the ___________.

Why does the S value alone not provide a measure of molecular mass?

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _; individual B cell clones which respond to the same antigen have membrane-bound antibodies with an affinity for __.

In the yeast two-hybrid assay, the _ domain and the _ domain of a transcription factor are fused to two different proteins. If the proteins interact with each other, the transcription factor can allow reporter gene expression.