Exam 18: Techniques in Cell and Molecular Biology

Which electron microscope technique is used to create a three-dimensional image of the surfaces of objects ranging in size from a virus to an animal's head?

The total magnification of a microscope is equal to __________.

How are probes labeled with a radioactive isotope detected?

For higher resolution cryo-EM studies, what has replaced the use of negatively stained specimens?

________ are atoms with the same number of protons but varying numbers of neutrons.

What is the general term used to describe a variety of techniques in which a mixture of dissolved components is separated as it moves through a porous matrix?

When DNA molecules from two different organisms are incubated together, some duplexes are formed by DNA strands from the two species. What indicates that such duplexes are less stable than those between strands from the same organism?

What does cloning of cDNAs allow?

In which method of transfection are cells treated with DNA that is bound to positively charged lipids, called cationic liposomes, that are capable of fusing with the lipid bilayer of the cell membrane and delivering the DNA to the cytoplasm?

Which of the following is a technique used to isolate a particular organelle in bulk so that its function can be studied or so that an enzyme can be isolated from it?

A ________ is a substance that reveals its presence in one way or another and thus can be localized or monitored during an experiment.

Which technique can be used for molecular weight (mass)determinations in proteins?

The amount of light passing through a solution unabsorbed (or _________)is measured by a ___________ on the other side of the cuvette.

Which of the following is most likely to elute first from a gel filtration column if each of them is essentially globular?

Which of the following is NOT a commonly used fluorophore?

What happens if a photographic emulsion is brought into close contact with a radioactive source in a specimen?

Why have frozen-hydrated particles largely replaced negatively stained specimens for high resolution cryo-EM studies?

When specimens are air-dried, what causes the damage that the tissues suffer?

Which form of radiation is emitted by the most commonly used isotopes and by most biologically important isotopes?

In phage display, the antibody molecule is displayed on the _______.