Exam 8: Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

Which of the following organelles imports proteins in their native, folded conformation?

What GTP-binding protein is associated with the formation of the COPI coat on COPI-coated vesicles?

A ____________ is thought to dissociate the 4-stranded SNARE complex by attaching to the SNARE bundle and, using energy from ATP hydrolysis, twisting it apart.

Which of the models below suggests that the Golgi cisternae are transient structures that form at the cis face of the stack by fusion of membranous carriers from the ER and ERGIC and that each cisterna travels through the Golgi complex from the cis to the trans end of the stack, changing in composition as it progresses?

What is the name for a brief incubation of a tissue with radioactivity during which labeled amino acids are incorporated into protein?

When homogenized, the endomembrane system is broken up into vesicles, which are heterogeneous but similar in size. These vesicles can be purified and, after purification, often retain their biological activity. They are collectively referred to as _________.

A control cell that is synthesizing a GFP-labeled version of mannosidase II has fluorescence localized in the numerous Golgi complexes of the cell. Normally, this enzyme is synthesized in the endoplasmic reticulum and moves via transport vesicles to the Golgi complex, where it takes up residence. What would an experimental cell look like if it contained an siRNA that led to the absence of one of the proteins involved in the transport of the enzyme from the ER to the Golgi complex? (Select all correct choices)

Which of the following is a difference between the coats of COPII- and clathrin-coated vesicles?

What are the two functional categories of SNAREs?

Vesicles that move through the Golgi complex from a trans-donor to a cis-acceptor membrane are said to move in a(n)__________ direction.

What initially happens if misfolded proteins are generated in the ER at a faster rate than they can be exported to the cytoplasm?

Phospholipids are made by integral ER membrane enzymes whose active sites face the cytosol and they are inserted into the outer (cytoplasmic)leaflet of the ER membrane. How then do lipids destined for the luminal leaflet of the ER membrane get there?

What happens if the UPR is unsuccessful in relieving the stressful conditions in the cell?

Which list below names the compartments into which chloroplast proteins can be imported?

Production and transport of materials in secretory vesicles which occurs continuously is known as:

In general, diseases that result from a deficiency of a single lysosomal enzyme are called ________.

How do protein coats select the cargo molecules to be carried by the vesicles they help to form?

What would happen to the movement of vesicles toward their eventual target if a microtubule inhibitor like colchicine were added to the cells?

The movement of vesicular-tubular carriers (VTCs)farther away from the ER and toward the Golgi complex occurs along tracks composed of what material?

Drugs that lower blood LDL levels are referred to as _______.