Exam 11: Genetic Engineering and Biotechnology

Artificially synthesizing DNA strands (e.g.,oligonucleotide primers)involves the careful attachment of one nucleotide at a time to an immobilized sequence.

Free

(True/False)

4.7/5

(26)

Question 1

Correct Answer:

Verified

True

One important advantage of eukaryotic cells as hosts for cloning vectors is that they already possess the complex RNA and posttranslational processing systems required for the production of eukaryotic proteins.

Free

(True/False)

4.8/5

(26)

Question 2

Correct Answer:

Verified

True

Explain how T7 promoters regulate transcription and why cloning vectors contain them.

Free

(Essay)

4.8/5

(39)

Question 3

Correct Answer:

Verified

The bacteriophage T7 polymerase gene is typically cloned on a vector such that it is under regulation of the lac promoter.In this way,analogs of lactose such as IPTG can be exogenously added to the growth medium at any given time to induce expression of the T7 polymerase.The T7 polymerase will recognize only T7 promoters,so only genes downstream to them are turned on in response to IPTG.Many cloning vectors contain T7 promoters upstream of the insert site so that their transcription can be easily manipulated.

The key steps in cloning a foreign gene into a vector,regardless of the application,involve isolating the insert fragment,ligating the insert into a vector,and transforming it into a host.

(True/False)

4.9/5

(37)

Question 4

If a protein to be overexpressed is toxic to the expression host,it is best to select an expression vector that

(Multiple Choice)

4.9/5

(28)

Question 5

What is necessary for a YAC to function as a normal eukaryotic chromosome?

(Essay)

4.8/5

(34)

Question 6

Engineering a metabolic pathway enables a researcher to use different genes from unrelated organisms.

(True/False)

4.8/5

(31)

Question 7

To verify a gene was cloned into a vector successfully,sequencing the vector as well as ________ are commonly performed.

(Multiple Choice)

4.9/5

(27)

Question 8

Some proteins overexpressed at high levels resulting in the formation of inclusion bodies can abolish the goal of producing large quantities of active protein.What could be done to minimize this issue?

(Multiple Choice)

4.8/5

(25)

Question 9

One fundamental technique of genetic engineering includes the ability to cut DNA into random fragments.

(True/False)

4.9/5

(31)

Question 10

If you wanted to discover a novel biosynthetic pathway for an antimicrobial compound and were given a handful of soil,describe the key experimental steps you would need to take to accomplish the task and explain why alternative procedures were not performed.

(Essay)

4.8/5

(28)

Question 11

Although various codons often code for the same amino acid,it is important to choose the codon preferred by the expression host itself.

(True/False)

4.9/5

(36)

Question 12

Explain why DNA fragments migrate toward the positive electrode and why some fragments migrate more rapidly than others during gel electrophoresis.How does this electronegativity influence observations and conclusions drawn from DNA migrated through an agarose gel?

(Essay)

4.8/5

(39)

Question 13

High expression levels of a eukaryotic gene in a bacterium such as Escherichia coli cannot be accomplished due to the presence of introns.

(True/False)

4.7/5

(36)

Question 14

The principle behind a nucleic acid probe design is that the probe itself must contain

(Multiple Choice)

4.7/5

(30)

Question 15

In principle,a type II restriction endonuclease with an 8-nucleotide recognition sequence should cut 1 in every 4⁸ nucleotide positions.

(True/False)

4.7/5

(45)

Question 16

Polyvalent vaccines using vaccinia virus are highly favored by doctors and physicians but are especially challenging for those who develop them,because

(Multiple Choice)

4.8/5

(38)

Question 17

What makes eukaryotic transcripts easier to isolate than transcripts in bacteria?

(Multiple Choice)

4.9/5

(40)

Question 18

Which construct would be MOST useful in studying translational control?

(Multiple Choice)

4.9/5

(34)

Question 19

Describe the usefulness of blue-white screening,also called α-complementation,in cloning vectors such as pUC19.Include in your answer the terms polylinker,DNA ligase,lacZ gene,insertional inactivation,Xgal,and β-galactosidase.

(Essay)

4.8/5

(40)

Question 20

Showing 1 - 20 of 81

Exam 11: Genetic Engineering and Biotechnology

Artificially synthesizing DNA strands (e.g.,oligonucleotide primers)involves the careful attachment of one nucleotide at a time to an immobilized sequence.

One important advantage of eukaryotic cells as hosts for cloning vectors is that they already possess the complex RNA and posttranslational processing systems required for the production of eukaryotic proteins.

Explain how T7 promoters regulate transcription and why cloning vectors contain them.

The key steps in cloning a foreign gene into a vector,regardless of the application,involve isolating the insert fragment,ligating the insert into a vector,and transforming it into a host.

If a protein to be overexpressed is toxic to the expression host,it is best to select an expression vector that

What is necessary for a YAC to function as a normal eukaryotic chromosome?

Engineering a metabolic pathway enables a researcher to use different genes from unrelated organisms.

To verify a gene was cloned into a vector successfully,sequencing the vector as well as ________ are commonly performed.

Some proteins overexpressed at high levels resulting in the formation of inclusion bodies can abolish the goal of producing large quantities of active protein.What could be done to minimize this issue?

One fundamental technique of genetic engineering includes the ability to cut DNA into random fragments.

If you wanted to discover a novel biosynthetic pathway for an antimicrobial compound and were given a handful of soil,describe the key experimental steps you would need to take to accomplish the task and explain why alternative procedures were not performed.

Although various codons often code for the same amino acid,it is important to choose the codon preferred by the expression host itself.

Explain why DNA fragments migrate toward the positive electrode and why some fragments migrate more rapidly than others during gel electrophoresis.How does this electronegativity influence observations and conclusions drawn from DNA migrated through an agarose gel?

High expression levels of a eukaryotic gene in a bacterium such as Escherichia coli cannot be accomplished due to the presence of introns.

The principle behind a nucleic acid probe design is that the probe itself must contain

In principle,a type II restriction endonuclease with an 8-nucleotide recognition sequence should cut 1 in every 4⁸ nucleotide positions.

Polyvalent vaccines using vaccinia virus are highly favored by doctors and physicians but are especially challenging for those who develop them,because

What makes eukaryotic transcripts easier to isolate than transcripts in bacteria?

Which construct would be MOST useful in studying translational control?

Describe the usefulness of blue-white screening,also called α-complementation,in cloning vectors such as pUC19.Include in your answer the terms polylinker,DNA ligase,lacZ gene,insertional inactivation,Xgal,and β-galactosidase.

Microorganisms and Microbiology

Microbial Cell Structure and Function

Microbial Metabolism

Molecular Biology

Microbial Growth and Control

Microbial Genomics

Metabolic Regulation

Viruses and Virology

Viral Genomes and Diversity

Genetics of Bacteria and Archaea

Microbial Evolution and Systematics

Metabolic Diversity of Microorganisms

Functional Diversity of Bacteria

Diversity of Bacteria

Diversity of Archaea

Diversity of Eukaryotic Microorganisms

Methods in Microbial Ecology

Microbial Ecosystems

Nutrient Cycles

Microbiology of the Built Environment

Microbial Symbioses

Microbial Interactions With Humans

Immunity and Host Defense

Immune Mechanisms

Molecular Immunology

Diagnostic Microbiology

Epidemiology

Person-To-Person Bacterial and Viral Diseases

Vectorborne and Soilborne Bacterial and Viral Diseases

Water and Food As Vehicles of Bacterial Diseases

Eukaryotic Pathogens: Fungal and Parasitic Diseases

Filters