Question 1

The uptake of DNA released from a cell is ________,while transfer of DNA with cell-to-cell contact would most likely result in ________.

Accepted Answer

A)  transformation / conjugation 
B)  transduction / conjugation 
C)  conjugation / transformation 
D)  transformation / transduction 
A)  transformation / conjugation 
B)  transduction / conjugation 
C)  conjugation / transformation 
D)  transformation / transduction A

Question 2

The process in which related DNA sequences from two different sources are exchanged is called

Accepted Answer

A)  transduction. 
B)  phage conversion. 
C)  reversion. 
D)  homologous recombination. 
A)  transduction. 
B)  phage conversion. 
C)  reversion. 
D)  homologous recombination. D

Question 3

Plasmids that govern their own transfer are known as

Accepted Answer

A)  transformable. 
B)  transmutable. 
C)  conjugative. 
D)  transfective. 
A)  transformable. 
B)  transmutable. 
C)  conjugative. 
D)  transfective. C

Question 4

A plasmid may

Accepted Answer

A)  replicate independently of the chromosome. 
B)  be transferred cell-to-cell during conjugation. 
C)  be integrated into the chromosome. 
D)  replicate independently of the chromosome, integrate into the chromosome, or be transferred cell-to-cell during conjugation. 
A)  replicate independently of the chromosome. 
B)  be transferred cell-to-cell during conjugation. 
C)  be integrated into the chromosome. 
D)  replicate independently of the chromosome, integrate into the chromosome, or be transferred cell-to-cell during conjugation.

Question 5

The enzyme transposase may be coded for by insertion sequences on a

Accepted Answer

A)  chromosome. 
B)  phage. 
C)  plasmid. 
D)  chromosome, phage, or plasmid. 
A)  chromosome. 
B)  phage. 
C)  plasmid. 
D)  chromosome, phage, or plasmid.

Question 6

F⁺ strains of Escherichia coli

Accepted Answer

A)  do not have an F factor. 
B)  have the F factor as a plasmid. 
C)  have an integrated F factor. 
D)  transfer the F factor to recipient cells at a high frequency. 
A)  do not have an F factor. 
B)  have the F factor as a plasmid. 
C)  have an integrated F factor. 
D)  transfer the F factor to recipient cells at a high frequency.

Question 7

Which of the following proteins is NOT required for homologous recombination?

Accepted Answer

A)  Hfr 
B)  RecA 
C)  proteins having helicase activity 
D)  endonuclease 
A)  Hfr 
B)  RecA 
C)  proteins having helicase activity 
D)  endonuclease

Question 8

Consider conjugation in Escherichia coli.In which of the following matings would chromosomal genes be transferred most frequently?

Accepted Answer

A)  F⁺ × F⁻ 
B)  F⁻ × F⁻ 
C)  Hfr × F⁻ 
D)  Hfr × F⁺ 
A)  F⁺ × F⁻ 
B)  F⁻ × F⁻ 
C)  Hfr × F⁻ 
D)  Hfr × F⁺

Question 9

All Hfr strains integrate into the chromosome at

Accepted Answer

A)  the same locus. 
B)  several specific sites. 
C)  the same locus most of the time, although there may be some variation. 
D)  loci that cannot be accurately determined. 
A)  the same locus. 
B)  several specific sites. 
C)  the same locus most of the time, although there may be some variation. 
D)  loci that cannot be accurately determined.

Question 10

In a prokaryotic genome,either insertion elements OR transposons are present,but both are never present at the same time.

Accepted Answer

A) True 
 B)False

Question 11

Transposition is a(n)

Accepted Answer

A)  homologous recombination event. 
B)  analogous recombination event. 
C)  site-specific recombination event. 
D)  general recombination event. 
A)  homologous recombination event. 
B)  analogous recombination event. 
C)  site-specific recombination event. 
D)  general recombination event.

Question 12

In specialized transduction,as exemplified by lambda phage in E.coli,transduction occurs at high efficiency for only a restricted group of genes near the insertion site of lambda.

Accepted Answer

A) True 
 B)False

Question 13

The SOS regulatory system is activated by

Accepted Answer

A)  the activity of DNA polymerase IV. 
B)  DNA damage. 
C)  transcription of LexA. 
D)  repression of RecA. 
A)  the activity of DNA polymerase IV. 
B)  DNA damage. 
C)  transcription of LexA. 
D)  repression of RecA.

Question 14

When damaged or single-stranded DNA activates the RecA protein,the RecA protein stimulates the cleavage of LexA.This results in

Accepted Answer

A)  repression of polymerase V and activation of endonuclease. 
B)  activation of the Hfr system. 
C)  derepression of the SOS system. 
D)  increased transduction and recombination. 
A)  repression of polymerase V and activation of endonuclease. 
B)  activation of the Hfr system. 
C)  derepression of the SOS system. 
D)  increased transduction and recombination.

Question 15

Homologous recombination has been observed in

Accepted Answer

A)  Archaea. 
B)  Bacteria. 
C)  Eukarya. 
D)  Archaea, Bacteria, and Eukarya. 
A)  Archaea. 
B)  Bacteria. 
C)  Eukarya. 
D)  Archaea, Bacteria, and Eukarya.

Question 16

A typical mutation rate for a bacterium is in the range of 10⁻⁶ to 10⁻⁹ per kbp.

Accepted Answer

A) True 
 B)False

Question 17

If a bacterium carrying a plasmid that confers resistance to ampicillin is placed into medium without ampicillin,it may

Accepted Answer

A)  gain resistance to other antibiotics. 
B)  transfer resistance to other cultures in the laboratory. 
C)  undergo a reversion mutation. 
D)  lose the plasmid because there is no selection for ampicillin resistance. 
A)  gain resistance to other antibiotics. 
B)  transfer resistance to other cultures in the laboratory. 
C)  undergo a reversion mutation. 
D)  lose the plasmid because there is no selection for ampicillin resistance.

Question 18

The F (fertility)plasmid contains a set of genes that encode for the ________ proteins that are essential in conjugative transfer of DNA.

Accepted Answer

A)  pili 
B)  SOS repair 
C)  transduction 
D)  transformation 
A)  pili 
B)  SOS repair 
C)  transduction 
D)  transformation

Question 19

You have performed the following mating experiment using Hfr and F⁻ strains of Escherichia coli: Hfr (thr⁺ leu⁺ gal⁺ strˢ)× F⁻ (thr⁻ leu⁻ gal⁻ strʳ).Which of the following selective media would you use to score recombinant colonies?

Accepted Answer

A)  minimal medium 
B)  minimal medium + streptomycin 
C)  minimal medium + threonine 
D)  minimal medium + streptomycin + threonine 
A)  minimal medium 
B)  minimal medium + streptomycin 
C)  minimal medium + threonine 
D)  minimal medium + streptomycin + threonine

Question 20

When UV radiation damage occurs,DNA repair occurs only in the absence of template instruction.

Accepted Answer

A) True 
 B)False

Exam 10: Genetics of Bacteria and Archaea

The uptake of DNA released from a cell is ________,while transfer of DNA with cell-to-cell contact would most likely result in ________.

The process in which related DNA sequences from two different sources are exchanged is called

Plasmids that govern their own transfer are known as

A plasmid may

The enzyme transposase may be coded for by insertion sequences on a

F⁺ strains of Escherichia coli

Which of the following proteins is NOT required for homologous recombination?

Consider conjugation in Escherichia coli.In which of the following matings would chromosomal genes be transferred most frequently?

All Hfr strains integrate into the chromosome at

In a prokaryotic genome,either insertion elements OR transposons are present,but both are never present at the same time.

Transposition is a(n)

In specialized transduction,as exemplified by lambda phage in E.coli,transduction occurs at high efficiency for only a restricted group of genes near the insertion site of lambda.

The SOS regulatory system is activated by

When damaged or single-stranded DNA activates the RecA protein,the RecA protein stimulates the cleavage of LexA.This results in

Homologous recombination has been observed in

A typical mutation rate for a bacterium is in the range of 10⁻⁶ to 10⁻⁹ per kbp.

If a bacterium carrying a plasmid that confers resistance to ampicillin is placed into medium without ampicillin,it may

The F (fertility)plasmid contains a set of genes that encode for the ________ proteins that are essential in conjugative transfer of DNA.

You have performed the following mating experiment using Hfr and F⁻ strains of Escherichia coli: Hfr (thr⁺ leu⁺ gal⁺ strˢ)× F⁻ (thr⁻ leu⁻ gal⁻ strʳ).Which of the following selective media would you use to score recombinant colonies?

When UV radiation damage occurs,DNA repair occurs only in the absence of template instruction.

Microorganisms and Microbiology

Microbial Cell Structure and Function

Microbial Metabolism

Molecular Biology

Microbial Growth and Control

Microbial Genomics

Metabolic Regulation

Viruses and Virology

Viral Genomes and Diversity

Genetic Engineering and Biotechnology

Microbial Evolution and Systematics

Metabolic Diversity of Microorganisms

Functional Diversity of Bacteria

Diversity of Bacteria

Diversity of Archaea

Diversity of Eukaryotic Microorganisms

Methods in Microbial Ecology

Microbial Ecosystems

Nutrient Cycles

Microbiology of the Built Environment

Microbial Symbioses

Microbial Interactions With Humans

Immunity and Host Defense

Immune Mechanisms

Molecular Immunology

Diagnostic Microbiology

Epidemiology

Person-To-Person Bacterial and Viral Diseases

Vectorborne and Soilborne Bacterial and Viral Diseases

Water and Food As Vehicles of Bacterial Diseases

Eukaryotic Pathogens: Fungal and Parasitic Diseases

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The uptake of DNA released from a cell is ,while transfer of DNA with cell-to-cell contact would most likely result in .