Exam 11: Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

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In the microdilution broth dilution method,the interpretive criteria for the susceptible,intermediate,or resistant categories are based on the:

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Assaying bacterial isolates for resistance genes by molecular methods:

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B

Chromogenic cephalosporins are used to test for:

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C

Daptomycin requires free calcium for its antibacterial action,is inhibited by pulmonary surfactant,and has activity against gram-positive bacteria.Gram-positive bacterial isolates can be tested for daptomycin susceptibility.For which type of infection should daptomycin susceptibility NOT be reported?

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AST was performed using Mueller-Hinton agar with 5% sheep blood,35°C in 5% CO₂ from 24 hours according to standard protocols,what is the most likely identity of the organism being tested?

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A bacterial suspension of 1.5× 10⁸ colony-forming units (CFUs)/mL is comparable with which McFarland standard?

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Staphylococcal resistance to oxacillin is used to determine and report resistance to:

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Antimicrobial susceptibility testing should be performed when

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The minimum inhibitory concentration (MIC)result recorded in antimicrobial testing can be defined as the:

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Detection of the haze produced by some resistant staphylococci and enterococci against methicillin and vancomycin can best be accomplished by:

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A sputum culture from a cystic fibrosis patient grew Stenotrophomonas maltophila.A broth microdilution panel was inoculated.Antimicrobials tested included: ceftazidime,imipenem,ticarcillin-clavulanate,levofloxacin,and trimethoprim-sulfamethoxazole.After 20-hour incubation at 35°C in ambient air,no growth was seen in any well of the microtiter plate.Which of the following statements is true?

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Enterococcal high-level gentamicin resistance predicts resistance to:

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The antibiotic nitrofurantoin is effective against both gram-positive and gram-negative bacteria.This antibiotic is effective against which type of specimen?

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Enterococcal resistance to ampicillin predicts resistance to:

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What action should be taken for any isolate found to be nonsusceptible (NS)to a particular antimicrobial agent?

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MIC endpoints can be reported results obtained by the following conventional AST methods EXCEPT:

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Which antimicrobial susceptibility testing method uses a series of plates with different antimicrobial concentrations inoculated with multiple bacterial isolates per plate?

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In the disk diffusion method of antimicrobial susceptibility testing,what is the standard medium used for testing most bacterial organisms?

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The minimal bactericidal concentration (MBC)test:

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S.pneumoniae was isolated from a spinal fluid sample from an inpatient.An oxacillin disk screening test was performed and the zone of inhibition measured 19 mm.Which action is most appropriate?

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