Question 1

H.influenzae can be distinguished from H.aegyptius by its _____ test for _____.

Accepted Answer

A) negative;sucrose fermentation 
B) negative;lactose fermentation 
C) positive;catalase 
D) positive;xylose fermentation 
A) negative;sucrose fermentation 
B) negative;lactose fermentation 
C) positive;catalase 
D) positive;xylose fermentation D

Question 2

Chancroid is the sexually transmitted disease caused by:

Accepted Answer

A) H.influenzae. 
B) Haemophilus ducreyi. 
C) H.aphrophilus. 
D) Haemophilus parainfluenzae. 
A) H.influenzae. 
B) Haemophilus ducreyi. 
C) H.aphrophilus. 
D) Haemophilus parainfluenzae. B

Question 3

In a phenomenon known as satelliting,tiny colonies of Haemophilus spp.may be observed growing on sheep blood agar close to colonies of bacteria that can produce:

Accepted Answer

A) hemin. 
B) NAD. 
C) vitamin K. 
D) cystine. 
A) hemin. 
B) NAD. 
C) vitamin K. 
D) cystine. B

Question 4

A 6-year-old boy was diagnosed with purulent conjunctivitis caused by a species of Haemophilus.What would the biochemical profile of the organism be?

Accepted Answer

A) Growth requirements require both X and V factors;the organism is hemolytic on rabbit blood. 
B) Growth requirements require both X and V factors;the organism is nonhemolytic on rabbit blood. 
C) Growth requirements require V factor;the organism is hemolytic on rabbit blood. 
D) Growth requirements require X factor;the organism is nonhemolytic on rabbit blood. 
A) Growth requirements require both X and V factors;the organism is hemolytic on rabbit blood. 
B) Growth requirements require both X and V factors;the organism is nonhemolytic on rabbit blood. 
C) Growth requirements require V factor;the organism is hemolytic on rabbit blood. 
D) Growth requirements require X factor;the organism is nonhemolytic on rabbit blood.

Question 5

Of the following media,which provides the NAD necessary for the growth of Haemophilus spp.?

Accepted Answer

A) 5% sheep blood agar 
B) Brain-heart infusion agar 
C) Chocolate agar 
D) Nutrient agar 
A) 5% sheep blood agar 
B) Brain-heart infusion agar 
C) Chocolate agar 
D) Nutrient agar

Question 6

Isolates from cerebrospinal fluid (CSF)or respiratory tract specimens that are gram-negative coccobacilli grow on chocolate agar in CO₂ but not on blood agar,and are porphyrin-negative and nonhemolytic on rabbit or horse blood may be identified as:

Accepted Answer

A) H.parainfluenzae. 
B) Haemophilus parahaemolyticus. 
C) H.haemolyticus. 
D) H.influenzae. 
A) H.parainfluenzae. 
B) Haemophilus parahaemolyticus. 
C) H.haemolyticus. 
D) H.influenzae.

Question 7

In a phenomenon known as satelliting,tiny colonies of Haemophilus spp.may be observed growing on sheep blood agar close to colonies of:

Accepted Answer

A) Streptococcus pyogenes. 
B) Staphylococcus aureus. 
C) Micrococcus spp. 
D) other Haemophilus spp. 
A) Streptococcus pyogenes. 
B) Staphylococcus aureus. 
C) Micrococcus spp. 
D) other Haemophilus spp.

Question 8

Although a useful test in the past,the particle agglutination assay for the detection of H.influenzae,type b,capsular polysaccharide antigen is no longer reliable because of:

Accepted Answer

A) insufficiently high concentration of the organisms in clinical infections. 
B) a lack of sensitivity and specificity of the test procedure. 
C) the frequent use of antimicrobial therapy before collecting the specimen. 
D) the increased use of H.influenzae,type b,vaccination. 
A) insufficiently high concentration of the organisms in clinical infections. 
B) a lack of sensitivity and specificity of the test procedure. 
C) the frequent use of antimicrobial therapy before collecting the specimen. 
D) the increased use of H.influenzae,type b,vaccination.

Question 9

Which of the following media provides the factors necessary for the growth of Haemophilus spp.?

Accepted Answer

A) 5% sheep blood agar 
B) Brain-heart infusion agar 
C) Chocolate agar 
D) Nutrient agar 
A) 5% sheep blood agar 
B) Brain-heart infusion agar 
C) Chocolate agar 
D) Nutrient agar

Question 10

To increase the sensitivity of direct Gram-stain examination of body fluid specimens,the specimens are usually:

Accepted Answer

A) incubated for 18 to 24 hours before staining. 
B) centrifuged before staining. 
C) examined for at least 1 hour. 
D) stained for an additional 5 minutes. 
A) incubated for 18 to 24 hours before staining. 
B) centrifuged before staining. 
C) examined for at least 1 hour. 
D) stained for an additional 5 minutes.

Question 11

The virulence of this organism,which causes chancroid,is most likely attributed to:

Accepted Answer

A) proteases. 
B) capsular factors. 
C) hemolysins. 
D) catalase. 
A) proteases. 
B) capsular factors. 
C) hemolysins. 
D) catalase.

Question 12

The sputum of a 75-year-old man with chronic lung infections yields the following:
·Many gram-negative coccobacilli in Gram stain
·X and V factors required for growth
·Nonhemolytic on rabbit blood agar
The organism can be identified as:

Accepted Answer

A) Haemophilus aegyptius. 
B) H.parainfluenzae. 
C) Haemophilus haemolyticus. 
D) H.influenzae. 
A) Haemophilus aegyptius. 
B) H.parainfluenzae. 
C) Haemophilus haemolyticus. 
D) H.influenzae.

Question 13

Which of the following opportunistic organisms is associated with slowly progressive subacute bacterial endocarditis?

Accepted Answer

A) H.ducreyi 
B) H.influenzae 
C) Aggregatibacter aphrophilus 
D) H.parahaemolyticus 
A) H.ducreyi 
B) H.influenzae 
C) Aggregatibacter aphrophilus 
D) H.parahaemolyticus

Question 14

Isolates from an ear swab that are gram-negative coccobacilli grow on chocolate agar in CO₂ but not blood agar,require both X and V factors for growth,and are nonhemolytic on rabbit or horse blood may be identified as:

Accepted Answer

A) H.parainfluenzae. 
B) H.ducreyi. 
C) H.aphrophilus. 
D) H.influenzae. 
A) H.parainfluenzae. 
B) H.ducreyi. 
C) H.aphrophilus. 
D) H.influenzae.

Question 15

Among Haemophilus influenzae strains,group designations (e.g. ,type b)are based on:

Accepted Answer

A) flagellar antigens. 
B) capsular characteristics. 
C) cell wall antigens. 
D) infectious capabilities. 
A) flagellar antigens. 
B) capsular characteristics. 
C) cell wall antigens. 
D) infectious capabilities.

Question 16

With one exception,species of the genus Haemophilus require which of the following media ingredients for in vitro growth?

Accepted Answer

A) Vitamin K and hemin 
B) Hemin and nicotine adenine dinucleotide (NA
D)  
C) Vitamin K and NAD 
D) Hemin and cysteine 
A) Vitamin K and hemin 
B) Hemin and nicotine adenine dinucleotide (NA
D)  
C) Vitamin K and NAD 
D) Hemin and cysteine

Question 17

Isolates from CSF that are gram-negative coccobacilli,grow on chocolate agar in CO₂ but not blood agar,require factor V but not X,and are nonhemolytic on rabbit or horse blood may be identified as:

Accepted Answer

A) H.parainfluenzae. 
B) H.influenzae. 
C) H.aphrophilus. 
D) H.ducreyi. 
A) H.parainfluenzae. 
B) H.influenzae. 
C) H.aphrophilus. 
D) H.ducreyi.

Question 18

Performance of the "factor requirement test" for Haemophilus involves the:

Accepted Answer

A) inoculation of unsupplemented media with a light suspension of the organism and placement of X and V factor disks on the agar surface. 
B) inoculation of liquid media,unsupplemented and supplemented with X and V factors. 
C) detection of the presence of enzymes that convert d-aminolevulinic acid (AL
A) into porphyrins. 
D) growth of the organism in the presence of bacterial species that produce X and V factors as metabolic byproducts. 
A) inoculation of unsupplemented media with a light suspension of the organism and placement of X and V factor disks on the agar surface. 
B) inoculation of liquid media,unsupplemented and supplemented with X and V factors. 
C) detection of the presence of enzymes that convert d-aminolevulinic acid (AL
A) into porphyrins. 
D) growth of the organism in the presence of bacterial species that produce X and V factors as metabolic byproducts.

Question 19

Growth of most Haemophilus species in culture is enhanced by:

Accepted Answer

A) incubation at 33°C to 35°C. 
B) high humidity. 
C) 5% to 10% carbon dioxide (CO₂). 
D) enriched special media. 
A) incubation at 33°C to 35°C. 
B) high humidity. 
C) 5% to 10% carbon dioxide (CO₂). 
D) enriched special media.

Question 20

H.ducreyi requires what special media to grow?

Accepted Answer

A) Heart infusion-based agar,supplemented with 10% IsoVitaleX and vancomycin 
B) Mueller-Hinton-based chocolate agar,supplemented with 1% IsoVitaleX and vancomycin 
C) Heart infusion-based chocolate agar,supplemented with 10% fetal bovine serum and vancomycin 
D) Mueller-Hinton-based agar,supplemented with 10% IsoVitaleX and vancomycin 
A) Heart infusion-based agar,supplemented with 10% IsoVitaleX and vancomycin 
B) Mueller-Hinton-based chocolate agar,supplemented with 1% IsoVitaleX and vancomycin 
C) Heart infusion-based chocolate agar,supplemented with 10% fetal bovine serum and vancomycin 
D) Mueller-Hinton-based agar,supplemented with 10% IsoVitaleX and vancomycin

Exam 30: Haemophilus

H.influenzae can be distinguished from H.aegyptius by its _____ test for _____.

Chancroid is the sexually transmitted disease caused by:

In a phenomenon known as satelliting,tiny colonies of Haemophilus spp.may be observed growing on sheep blood agar close to colonies of bacteria that can produce:

A 6-year-old boy was diagnosed with purulent conjunctivitis caused by a species of Haemophilus.What would the biochemical profile of the organism be?

Of the following media,which provides the NAD necessary for the growth of Haemophilus spp.?

Isolates from cerebrospinal fluid (CSF)or respiratory tract specimens that are gram-negative coccobacilli grow on chocolate agar in CO₂ but not on blood agar,and are porphyrin-negative and nonhemolytic on rabbit or horse blood may be identified as:

In a phenomenon known as satelliting,tiny colonies of Haemophilus spp.may be observed growing on sheep blood agar close to colonies of:

Although a useful test in the past,the particle agglutination assay for the detection of H.influenzae,type b,capsular polysaccharide antigen is no longer reliable because of:

Which of the following media provides the factors necessary for the growth of Haemophilus spp.?

To increase the sensitivity of direct Gram-stain examination of body fluid specimens,the specimens are usually:

The virulence of this organism,which causes chancroid,is most likely attributed to:

The sputum of a 75-year-old man with chronic lung infections yields the following: ·Many gram-negative coccobacilli in Gram stain ·X and V factors required for growth ·Nonhemolytic on rabbit blood agar The organism can be identified as:

Which of the following opportunistic organisms is associated with slowly progressive subacute bacterial endocarditis?

Isolates from an ear swab that are gram-negative coccobacilli grow on chocolate agar in CO₂ but not blood agar,require both X and V factors for growth,and are nonhemolytic on rabbit or horse blood may be identified as:

Among Haemophilus influenzae strains,group designations (e.g. ,type b)are based on:

With one exception,species of the genus Haemophilus require which of the following media ingredients for in vitro growth?

Isolates from CSF that are gram-negative coccobacilli,grow on chocolate agar in CO₂ but not blood agar,require factor V but not X,and are nonhemolytic on rabbit or horse blood may be identified as:

Performance of the "factor requirement test" for Haemophilus involves the:

Growth of most Haemophilus species in culture is enhanced by:

H.ducreyi requires what special media to grow?

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H.influenzae can be distinguished from H.aegyptius by its _ test for _.