Question 1

The determination of carbohydrate utilization patterns for the differentiation of Neisseria spp.and M.catarrhalis is best performed by:

Accepted Answer

A) incubation of inoculated cysteine trypticase soy agar (CT
A) with 1% dextrose,maltose,lactose,and sucrose for 48 to 72 hours. 
B) oxidative-fermentative (OF)medium with dextrose,maltose,lactose,and sucrose. 
C) heavy inoculation of buffered,low-peptone substrate with dextrose,maltose,lactose,and sucrose. 
D) carbohydrate testing strips. 
A) incubation of inoculated cysteine trypticase soy agar (CT
A) with 1% dextrose,maltose,lactose,and sucrose for 48 to 72 hours. 
B) oxidative-fermentative (OF)medium with dextrose,maltose,lactose,and sucrose. 
C) heavy inoculation of buffered,low-peptone substrate with dextrose,maltose,lactose,and sucrose. 
D) carbohydrate testing strips. C

Question 2

The gram-negative coccus that can be described as oxidase-positive,glucose-negative,maltose-negative,sucrose-negative,lactose-negative,and DNase-positive is:

Accepted Answer

A) N.meningitidis. 
B) M.catarrhalis. 
C) Neisseria cinerea. 
D) N.gonorrhoeae. 
A) N.meningitidis. 
B) M.catarrhalis. 
C) Neisseria cinerea. 
D) N.gonorrhoeae. B

Question 3

M.catarrhalis may be described as:

Accepted Answer

A) oxidase-positive. 
B) a glucose utilizer. 
C) a major cause of venereal disease. 
D) often carrying an antiphagocytic capsule. 
A) oxidase-positive. 
B) a glucose utilizer. 
C) a major cause of venereal disease. 
D) often carrying an antiphagocytic capsule. A

Question 4

Which one of the following statements is true regarding antimicrobial therapy and susceptibility testing?

Accepted Answer

A) Cephalosporins and macrolides are ineffective against M.catarrhalis. 
B) Resistance to quinolones is emerging in strains of N.gonorrhoeae. 
C) Most Neisseria spp.are resistant to penicillin. 
D) Most strains of N.meningitidis are resistant to penicillin. 
A) Cephalosporins and macrolides are ineffective against M.catarrhalis. 
B) Resistance to quinolones is emerging in strains of N.gonorrhoeae. 
C) Most Neisseria spp.are resistant to penicillin. 
D) Most strains of N.meningitidis are resistant to penicillin.

Question 5

Organisms belonging to the genus Neisseria are:

Accepted Answer

A) gram-positive diplococci. 
B) gram-negative diplococci. 
C) gram-negative coccobacilli. 
D) gram-negative bacilli. 
A) gram-positive diplococci. 
B) gram-negative diplococci. 
C) gram-negative coccobacilli. 
D) gram-negative bacilli.

Question 6

An advantage of all molecular assays is:

Accepted Answer

A) the ability to test for C.trachomatis from the same specimen at the same time. 
B) the ability to use these tests for assessing the successfulness of treatment. 
C) their role as evidence in medicolegal cases. 
D) the ability to detect the capsular antigen in body fluids. 
A) the ability to test for C.trachomatis from the same specimen at the same time. 
B) the ability to use these tests for assessing the successfulness of treatment. 
C) their role as evidence in medicolegal cases. 
D) the ability to detect the capsular antigen in body fluids.

Question 7

The second most commonly reported sexually transmitted infection in the United States,according to the CDC,is:

Accepted Answer

A) M.catarrhalis. 
B) N.gonorrhoeae. 
C) N.meningitidis. 
D) HIV. 
A) M.catarrhalis. 
B) N.gonorrhoeae. 
C) N.meningitidis. 
D) HIV.

Question 8

Gonococci require which of the following nutrients for growth?

Accepted Answer

A) Arginine 
B) Hypoxanthine 
C) Uracil 
D) IsoVitaleX 
A) Arginine 
B) Hypoxanthine 
C) Uracil 
D) IsoVitaleX

Question 9

Gram-negative diplococci are isolated from a genital specimen and yield the following results:
·Glucose: positive
·Maltose: negative
·Lactose: negative
The organism can be identified as:

Accepted Answer

A) N.gonorrhoeae. 
B) Neisseria meningitidis. 
C) Neisseria lactamica. 
D) M.catarrhalis. 
A) N.gonorrhoeae. 
B) Neisseria meningitidis. 
C) Neisseria lactamica. 
D) M.catarrhalis.

Question 10

Which two organisms should be used to determine the validity of the nitrate reduction test?

Accepted Answer

A) N.gonorrhoeae and N.lactamica 
B) Neisseria mucosa and M.catarrhalis 
C) N.lactamica and M.catarrhalis 
D) Neisseria sicca and Neisseria subflava 
A) N.gonorrhoeae and N.lactamica 
B) Neisseria mucosa and M.catarrhalis 
C) N.lactamica and M.catarrhalis 
D) Neisseria sicca and Neisseria subflava

Question 11

The differentiation of the similar M.catarrhalis and Neisseria can be best accomplished by:

Accepted Answer

A) examination of Gram stain of the colony. 
B) vancomycin susceptibility testing. 
C) the oxidase test. 
D) carbohydrate utilization testing. 
A) examination of Gram stain of the colony. 
B) vancomycin susceptibility testing. 
C) the oxidase test. 
D) carbohydrate utilization testing.

Question 12

A gram-negative diplococcus grew on 5% sheep blood agar and chocolate agar,had scant growth on MacConkey agar,and had no growth on modified Thayer-Martin (MTM)agar.The organism was identified as N.sicca.Which one of the following statements explains the discrepancy of the results obtained on the MTM agar?

Accepted Answer

A) The medium was most likely expired. 
B) The organism was most likely incorrectly transported. 
C) The organism was most likely incubated at 35°C instead of 25°C. 
D) No discrepancy is present.N.sicca does not grow on MTM agar. 
A) The medium was most likely expired. 
B) The organism was most likely incorrectly transported. 
C) The organism was most likely incubated at 35°C instead of 25°C. 
D) No discrepancy is present.N.sicca does not grow on MTM agar.

Question 13

N.gonorrhoeae is spread by:

Accepted Answer

A) person-to-person nosocomial spread by respiratory droplets. 
B) person-to-person spread by sexual contact. 
C) from infected mother to newborn during birth. 
D) B and C only. 
A) person-to-person nosocomial spread by respiratory droplets. 
B) person-to-person spread by sexual contact. 
C) from infected mother to newborn during birth. 
D) B and C only.

Question 14

An endocervical swab was collected from a 26-year-old woman with a purulent discharge.The Gram stain revealed the following:
·Many white blood cells (WBCs)
·Moderate gram-negative diplococci
·Moderate gram-negative coccobacilli
·Few yeast
Based on these findings,what would be the next course of action?

Accepted Answer

A) Report the isolation of Neisseria gonorrhoeae. 
B) Work up the yeast. 
C) Report the Gram stain,and follow up with culture. 
D) Request a new specimen;this one is contaminated. 
A) Report the isolation of Neisseria gonorrhoeae. 
B) Work up the yeast. 
C) Report the Gram stain,and follow up with culture. 
D) Request a new specimen;this one is contaminated.

Question 15

Blood cultures collected for the recovery of N.gonorrhoeae or N.meningitidis require:

Accepted Answer

A) no special methods. 
B) more than 0.025% sodium polyanethol sulfonate (SPS). 
C) transfer from Vacutainer tubes to culture broth within 1 hour of collection. 
D) less than 0.025% SPS. 
A) no special methods. 
B) more than 0.025% sodium polyanethol sulfonate (SPS). 
C) transfer from Vacutainer tubes to culture broth within 1 hour of collection. 
D) less than 0.025% SPS.

Question 16

An organism isolated from a sputum sample that has a "hockey puck" consistency would have which of the following biochemical profiles?

Accepted Answer

A) Glucose-positive,maltose-positive,and lactose-positive 
B) Glucose-positive,maltose-positive,and lactose-negative 
C) Glucose-positive,maltose-negative,and lactose-negative 
D) Glucose-negative,maltose-negative,and lactose-negative 
A) Glucose-positive,maltose-positive,and lactose-positive 
B) Glucose-positive,maltose-positive,and lactose-negative 
C) Glucose-positive,maltose-negative,and lactose-negative 
D) Glucose-negative,maltose-negative,and lactose-negative

Question 17

Which statement is true regarding the pathogenesis of N.cinerea?

Accepted Answer

A) N.cinerea is a leading cause of fatal bacterial meningitis. 
B) Virulence factors include pili,capsule formation,and endotoxin. 
C) Disseminated intravascular coagulation (DI
C) and thrombocytopenia are complications of infection. 
D) N.cinerea is commonly encountered as a contaminant in clinical specimens. 
A) N.cinerea is a leading cause of fatal bacterial meningitis. 
B) Virulence factors include pili,capsule formation,and endotoxin. 
C) Disseminated intravascular coagulation (DI
C) and thrombocytopenia are complications of infection. 
D) N.cinerea is commonly encountered as a contaminant in clinical specimens.

Question 18

MTM,Martin-Lewis (ML),and New York City (NYC)media were all developed for the selective isolation of which organism?

Accepted Answer

A) M.catarrhalis 
B) Chlamydia trachomatis 
C) N.gonorrhoeae 
D) N.mucosa 
A) M.catarrhalis 
B) Chlamydia trachomatis 
C) N.gonorrhoeae 
D) N.mucosa

Question 19

M.catarrhalis can be described as a:

Accepted Answer

A) gram-positive diplococcus. 
B) gram-negative diplococcus. 
C) gram-negative coccobacillus. 
D) gram-negative bacillus. 
A) gram-positive diplococcus. 
B) gram-negative diplococcus. 
C) gram-negative coccobacillus. 
D) gram-negative bacillus.

Question 20

M.catarrhalis may be differentiated from gonococci and meningococci on the basis of its:

Accepted Answer

A) growth on blood agar at 35°C. 
B) growth on nutrient agar at 22°C. 
C) reduction of nitrite to nitrate. 
D) production of DNase. 
A) growth on blood agar at 35°C. 
B) growth on nutrient agar at 22°C. 
C) reduction of nitrite to nitrate. 
D) production of DNase.

Exam 38: Neisseria and Moraxella Catarrhalis

The determination of carbohydrate utilization patterns for the differentiation of Neisseria spp.and M.catarrhalis is best performed by:

The gram-negative coccus that can be described as oxidase-positive,glucose-negative,maltose-negative,sucrose-negative,lactose-negative,and DNase-positive is:

M.catarrhalis may be described as:

Which one of the following statements is true regarding antimicrobial therapy and susceptibility testing?

Organisms belonging to the genus Neisseria are:

An advantage of all molecular assays is:

The second most commonly reported sexually transmitted infection in the United States,according to the CDC,is:

Gonococci require which of the following nutrients for growth?

Gram-negative diplococci are isolated from a genital specimen and yield the following results: ·Glucose: positive ·Maltose: negative ·Lactose: negative The organism can be identified as:

Which two organisms should be used to determine the validity of the nitrate reduction test?

The differentiation of the similar M.catarrhalis and Neisseria can be best accomplished by:

N.gonorrhoeae is spread by:

Blood cultures collected for the recovery of N.gonorrhoeae or N.meningitidis require:

An organism isolated from a sputum sample that has a "hockey puck" consistency would have which of the following biochemical profiles?

Which statement is true regarding the pathogenesis of N.cinerea?

MTM,Martin-Lewis (ML),and New York City (NYC)media were all developed for the selective isolation of which organism?

M.catarrhalis can be described as a:

M.catarrhalis may be differentiated from gonococci and meningococci on the basis of its:

Microbial Taxonomy

Bacterial Genetics, metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Overview of Immunochemical Methods Used for Organism Detection

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

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Sphingomonas Paucimobilis and Similar Organisms

Moraxella

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Overview and General Considerations

Laboratory Considerations

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Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Overview of the Methods and Strategies in Parasitology

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Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Zygomycetes, Dermatophytes, and Opportunistic and Systemic Mycoses

Dematiaceious Molds

Opportunistic Atypical Fungus: Pjirovecii

The Yeasts

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