Question 1

Which subunit of E. coli RNA polymerase is responsible for DNA binding?

Accepted Answer

A) $\alpha$ 
B) $\beta$ 
C) $\beta$' 
D)  $\sigma$ 
E) $\omega$ 
A) $\alpha$ 
B) $\beta$ 
C) $\beta$' 
D)  $\sigma$ 
E) $\omega$

Question 2

The figure below shows a newly transcribed mRNA still associated with the template DNA. Draw the stem-loop structure that often forms during termination.
3'-GGTCGGGCGGATTACTCGCCCGAAAAAAAA-5'
5'-CCAGCCCGCCUAAUGAGCGGGCUUUUUUUU-3'

Accepted Answer

AAU
U G
C A
C-G
G-C

Question 3

E. coli cells achieve a great deal of gene regulatory expression through the  $\sigma$subunit of RNA polymerase. Which  $\sigma$ subunit is present most often since it promotes the production of most of the proteins required under normal conditions within the cell?

Accepted Answer

A)  $\sigma$N 
B)  $\sigma$S 
C)  $\sigma$H 
D)  $\sigma$30 
E)  $\sigma$70 
A)  $\sigma$N 
B)  $\sigma$S 
C)  $\sigma$H 
D)  $\sigma$30 
E)  $\sigma$70

Question 4

Which of the following events commonly occur in the processing of eukaryotic mRNA?

Accepted Answer

A) addition of a 5' cap 
B) RNA editing 
C) splicing to remove introns 
D) alternative splicing to combine different exons into mature mRNA 
E) all of the above 
A) addition of a 5' cap 
B) RNA editing 
C) splicing to remove introns 
D) alternative splicing to combine different exons into mature mRNA 
E) all of the above

Question 5

In vivo, which of the following transcription initiation factors is required for binding to a TATA box?

Accepted Answer

A) TFIID 
B) TFIIE 
C) TFIIF 
D) TFIIH 
E) TAF 
A) TFIID 
B) TFIIE 
C) TFIIF 
D) TFIIH 
E) TAF

Which subunit of E. coli RNA polymerase is responsible for DNA binding?

The figure below shows a newly transcribed mRNA still associated with the template DNA. Draw the stem-loop structure that often forms during termination. 3'-GGTCGGGCGGATTACTCGCCCGAAAAAAAA-5' 5'-CCAGCCCGCCUAAUGAGCGGGCUUUUUUUU-3'

E. coli cells achieve a great deal of gene regulatory expression through the $\sigma$ subunit of RNA polymerase. Which $\sigma$ subunit is present most often since it promotes the production of most of the proteins required under normal conditions within the cell?

Which of the following events commonly occur in the processing of eukaryotic mRNA?

In vivo, which of the following transcription initiation factors is required for binding to a TATA box?

The Scope of Biochemistry

The Matrix of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Contractile Proteins and Molecular Motors

Carbohydrates: Sugars, Saccharides, Glycans

Lipids, Membranes and Cellular Transport

Enzymes: Biological Catalysts

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway

Citric Acid Cycle and Glyoxylate Cycle

Electron Transport, Oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism I: Fatty Acids, Triacylglycerols, and Lipoproteins

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Lipid Metabolism Ii: Membrane Lipids, Steroids, Isoprenoids, and Eicosanoids

Metabolism of Nitrogenous Compounds I: Principles of Biosynthesis, Utilization, and Turnover

Metabolism of Nitogenous Compounds II: Amino Acids, Porphyrins, and Neurotransmitters

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes, Genomes and Chromosomes

DNA Replication

DNA Restructuring: Repair, Recombination, Rearrangement, Amplification

Information Decoding: Translation and Post-Translational Protein Processing

Regulation of Gene Expression

Filters

Exam 27: Information Readout: Transcription and Post-Transcriptional Processing

Which subunit of E. coli RNA polymerase is responsible for DNA binding?

The figure below shows a newly transcribed mRNA still associated with the template DNA. Draw the stem-loop structure that often forms during termination. 3'-GGTCGGGCGGATTACTCGCCCGAAAAAAAA-5' 5'-CCAGCCCGCCUAAUGAGCGGGCUUUUUUUU-3'

E. coli cells achieve a great deal of gene regulatory expression through the σ\sigmaσ subunit of RNA polymerase. Which σ\sigmaσ subunit is present most often since it promotes the production of most of the proteins required under normal conditions within the cell?

Which of the following events commonly occur in the processing of eukaryotic mRNA?

In vivo, which of the following transcription initiation factors is required for binding to a TATA box?

The Scope of Biochemistry

The Matrix of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Contractile Proteins and Molecular Motors

Carbohydrates: Sugars, Saccharides, Glycans

Lipids, Membranes and Cellular Transport

Enzymes: Biological Catalysts

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway

Citric Acid Cycle and Glyoxylate Cycle

Electron Transport, Oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism I: Fatty Acids, Triacylglycerols, and Lipoproteins

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Lipid Metabolism Ii: Membrane Lipids, Steroids, Isoprenoids, and Eicosanoids

Metabolism of Nitrogenous Compounds I: Principles of Biosynthesis, Utilization, and Turnover

Metabolism of Nitogenous Compounds II: Amino Acids, Porphyrins, and Neurotransmitters

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes, Genomes and Chromosomes

DNA Replication

DNA Restructuring: Repair, Recombination, Rearrangement, Amplification

Information Decoding: Translation and Post-Translational Protein Processing

Regulation of Gene Expression

Filters

E. coli cells achieve a great deal of gene regulatory expression through the $\sigma$ subunit of RNA polymerase. Which $\sigma$ subunit is present most often since it promotes the production of most of the proteins required under normal conditions within the cell?