Question 1

If both lactose and glucose are present in large amounts,which of the following scenarios would result?

Accepted Answer

A) lac repressor bound to promoter,CRP bound to promoter,no lac operon transcription 
B) lac repressor bound to inducer,CRP bound to promoter,no lac operon transcription 
C) lac repressor bound to inducer,CRP bound to promoter,lac operon transcribed 
D) lac repressor bound to inducer,CRP not bound,no lac operon transcription 
E) lac repressor bound to inducer,CRP not bound,lac operon transcribed 
A) lac repressor bound to promoter,CRP bound to promoter,no lac operon transcription 
B) lac repressor bound to inducer,CRP bound to promoter,no lac operon transcription 
C) lac repressor bound to inducer,CRP bound to promoter,lac operon transcribed 
D) lac repressor bound to inducer,CRP not bound,no lac operon transcription 
E) lac repressor bound to inducer,CRP not bound,lac operon transcribed D

Question 2

The bromodomain of nucleosome remodeling complexes binds to ____________ histones while the chromodomain binds to ___________ histones.

Accepted Answer

A) acetylated;methylated 
B) acetylated;phosphorylated 
C) methylated;acetylated 
D) methylated;phosphorylated 
E) none of the above 
A) acetylated;methylated 
B) acetylated;phosphorylated 
C) methylated;acetylated 
D) methylated;phosphorylated 
E) none of the above A

Question 3

DNA damage that requires RecA to bind to a DNA gap activates proteolysis of _____,which then activates the transcription of genes within the SOS regulon.

Accepted Answer

A) cI 
B) Cro 
C) LexA 
D) uvrA 
E) uvrB 
A) cI 
B) Cro 
C) LexA 
D) uvrA 
E) uvrB C

Question 4

A short RNA that is complimentary to a specific mRNA and used for inhibition of translation is called ______________.

Accepted Answer

A) antisense RNA 
B) missense RNA 
C) nonsense RNA 
D) senseless RNA 
E) none of the above 
A) antisense RNA 
B) missense RNA 
C) nonsense RNA 
D) senseless RNA 
E) none of the above

Question 5

Which of the following accounts for the greater complexity in eukaryotic transcriptional control when compared with prokaryotic control?

Accepted Answer

A) physical separation of transcription from translation 
B) the presence of chromatin instead of naked DNA 
C) the larger size of the chromosomes 
D) the presence of introns 
E) none of the above 
A) physical separation of transcription from translation 
B) the presence of chromatin instead of naked DNA 
C) the larger size of the chromosomes 
D) the presence of introns 
E) none of the above

Question 6

What elements are contained within the promoter of the lactose operon?

Accepted Answer

A) operator and CRP site 
B) operator and repressor 
C) CRP site and repressor 
D) operator,CRP site and repressor 
E) none of the above 
A) operator and CRP site 
B) operator and repressor 
C) CRP site and repressor 
D) operator,CRP site and repressor 
E) none of the above

Question 7

Which of the following is the true inducer that binds to the lac repressor in vivo?

Accepted Answer

A) lactose 
B) glucose 
C) galactose 
D) isopropyl-$\beta$-thiogalactoside 
E) allolactose 
A) lactose 
B) glucose 
C) galactose 
D) isopropyl-$\beta$-thiogalactoside 
E) allolactose

Question 8

Which of the following bacteriophage $\lambda$ proteins serves as both an activator and a repressor?

Accepted Answer

A) cI 
B) cII 
C) cIII 
D) Cro 
E) N 
A) cI 
B) cII 
C) cIII 
D) Cro 
E) N

Question 9

On the polycistronic mRNA of bacteriophage MS2,the ability to translate multiple copies of the coat protein but only a single copy of the A protein is accomplished by _________.

Accepted Answer

A) regulatory RNA molecules 
B) translational repression by protein binding to mRNA 
C) antisense RNA 
D) ribosome binding site occlusion 
E) none of the above 
A) regulatory RNA molecules 
B) translational repression by protein binding to mRNA 
C) antisense RNA 
D) ribosome binding site occlusion 
E) none of the above

Question 10

The phosphorylation of several RNA polymerase II serine residues has been identified as a key event in eukaryotic translation initiation.Which of the following is one of the enzymes responsible for this phosphorylation?

Accepted Answer

A) Mediator complex 
B) Rap1 
C) TFIIH 
D) TATA box-binding protein 
E) ACT 
A) Mediator complex 
B) Rap1 
C) TFIIH 
D) TATA box-binding protein 
E) ACT

Question 11

In prokaryotes,DNA methylation occurs on ______ while in eukaryotes,methylation occurs on _______.

Accepted Answer

A) adenine;thymine 
B) adenine;cytosine 
C) guanine;adenine 
D) guanine;thymine 
E) guanine;cytosine 
A) adenine;thymine 
B) adenine;cytosine 
C) guanine;adenine 
D) guanine;thymine 
E) guanine;cytosine

Question 12

Give the sequence and draw the first two residues of a morpholino oligo that would serve as an antisense RNA for the following mRNA sequence (you may use the letter A,C,G and U to denote the bases):
5'-CAUGUCC-3'

Accepted Answer

The answer of Give the sequence and draw the first...

Question 13

What is thought to occur when CRP binds to DNA?

Accepted Answer

A) formation of a hairpin that prevents progression of RNA polymerase 
B) bending of DNA to facilitate RNA polymerase binding 
C) blocking of promoter regions to prevent binding of RNA polymerase 
D) forms non-covalent interactions with RNA polymerase to bind it to DNA 
E) none of the above 
A) formation of a hairpin that prevents progression of RNA polymerase 
B) bending of DNA to facilitate RNA polymerase binding 
C) blocking of promoter regions to prevent binding of RNA polymerase 
D) forms non-covalent interactions with RNA polymerase to bind it to DNA 
E) none of the above

Question 14

DNA methylation in eukaryotes plays a role in ____________.

Accepted Answer

A) methyl-directed mismatch repair 
B) protection of restriction sites 
C) chromatin remodeling 
D) cancerous cell growth 
E) gene silencing 
A) methyl-directed mismatch repair 
B) protection of restriction sites 
C) chromatin remodeling 
D) cancerous cell growth 
E) gene silencing

Question 15

In a histone protein,what modification to lysine marks the nucleosome as a transcription target?

Accepted Answer

A) phosphorylation 
B) methylation 
C) ubiquitylation 
D) ADP-ribosylation 
E) acetylation 
A) phosphorylation 
B) methylation 
C) ubiquitylation 
D) ADP-ribosylation 
E) acetylation

Question 16

If an miRNA is completely complementary to an mRNA,what is the result of the pairing of these two molecules?

Accepted Answer

A) rapid translation of the mRNA 
B) repeated translation as the miRNA prevents destruction of the mRNA 
C) slowed translation of the mRNA 
D) degradation of the mRNA 
E) none of the above 
A) rapid translation of the mRNA 
B) repeated translation as the miRNA prevents destruction of the mRNA 
C) slowed translation of the mRNA 
D) degradation of the mRNA 
E) none of the above

Question 17

Which of the genes in the SOS regulon encodes a DNA polymerase?

Accepted Answer

A) recA 
B) lexA 
C) uvrA 
D) uvrB 
E) dinA 
A) recA 
B) lexA 
C) uvrA 
D) uvrB 
E) dinA

Question 18

When bacteriophage $\lambda$ lysogeny terminates and lytic growth in E.coli cells begins,____ is inactivated leading to unoccupied OR sites._____ is produced,ultimately resulting in DNA replication.

Accepted Answer

A) Cro;cI 
B) cI;Cro 
C) cII;Cro 
D) cI;cII 
E) none of the above 
A) Cro;cI 
B) cI;Cro 
C) cII;Cro 
D) cI;cII 
E) none of the above

Question 19

Lysine methylation in a histone protein produces a form of chromatin known as ________ with the genes being transcriptionally __________.

Accepted Answer

A) euchromatin;active 
B) euchromatin;inactive 
C) heterochromatin;active 
D) heterochromatin;inactive 
E) none of the above 
A) euchromatin;active 
B) euchromatin;inactive 
C) heterochromatin;active 
D) heterochromatin;inactive 
E) none of the above

Question 20

Design an antisense RNA that prevents the start of translation for any mRNA in an E.coli cell.

Accepted Answer

In order to prevent the start of transla

Exam 29: Regulation of Gene Expression

If both lactose and glucose are present in large amounts,which of the following scenarios would result?

The bromodomain of nucleosome remodeling complexes binds to ____________ histones while the chromodomain binds to ___________ histones.

DNA damage that requires RecA to bind to a DNA gap activates proteolysis of _____,which then activates the transcription of genes within the SOS regulon.

A short RNA that is complimentary to a specific mRNA and used for inhibition of translation is called ______________.

Which of the following accounts for the greater complexity in eukaryotic transcriptional control when compared with prokaryotic control?

What elements are contained within the promoter of the lactose operon?

Which of the following is the true inducer that binds to the lac repressor in vivo?

Which of the following bacteriophage λ\lambdaλ proteins serves as both an activator and a repressor?

On the polycistronic mRNA of bacteriophage MS2,the ability to translate multiple copies of the coat protein but only a single copy of the A protein is accomplished by _________.

The phosphorylation of several RNA polymerase II serine residues has been identified as a key event in eukaryotic translation initiation.Which of the following is one of the enzymes responsible for this phosphorylation?

In prokaryotes,DNA methylation occurs on ______ while in eukaryotes,methylation occurs on _______.

Give the sequence and draw the first two residues of a morpholino oligo that would serve as an antisense RNA for the following mRNA sequence (you may use the letter A,C,G and U to denote the bases): 5'-CAUGUCC-3'

What is thought to occur when CRP binds to DNA?

DNA methylation in eukaryotes plays a role in ____________.

In a histone protein,what modification to lysine marks the nucleosome as a transcription target?

If an miRNA is completely complementary to an mRNA,what is the result of the pairing of these two molecules?

Which of the genes in the SOS regulon encodes a DNA polymerase?

When bacteriophage λ\lambdaλ lysogeny terminates and lytic growth in E.coli cells begins,____ is inactivated leading to unoccupied OR sites._____ is produced,ultimately resulting in DNA replication.

Lysine methylation in a histone protein produces a form of chromatin known as ________ with the genes being transcriptionally __________.

Design an antisense RNA that prevents the start of translation for any mRNA in an E.coli cell.

The Scope of Biochemistry

The Matrix of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Contractile Proteins and Molecular Motors

Carbohydrates: Sugars,saccharides,glycans

Lipids,membranes and Cellular Transport

Enzymes: Biological Catalysts

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, gluconeogenesis, glycogen Metabolism, and the Pentose Phosphate Pathway

Citric Acid Cycle and Glyoxylate Cycle

Electron Transport, oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism I: Fatty Acids,triacylglycerols,and Lipoproteins

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Lipid Metabolism Ii: Membrane Lipids, steroids, isoprenoids, and Eicosanoids

Metabolism of Nitrogenous Compounds I: Principles of Biosynthesis, utilization, and Turnover

Metabolism of Nitogenous Compounds Ii: Amino Acids, porphyrins, and Neurotransmitters

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes,genomes and Chromosomes

Dna Replication

Dna Restructuring: Repair,recombination,rearrangement,amplification

Information Readout: Transcription and Post-Transcriptional Processing

Information Decoding: Translation and Post-Translational Protein Processing

Filters

The bromodomain of nucleosome remodeling complexes binds to __ histones while the chromodomain binds to _ histones.

Which of the following bacteriophage $\lambda$ proteins serves as both an activator and a repressor?

In prokaryotes,DNA methylation occurs on while in eukaryotes,methylation occurs on _.

When bacteriophage $\lambda$ lysogeny terminates and lytic growth in E.coli cells begins,____ is inactivated leading to unoccupied O_R sites._____ is produced,ultimately resulting in DNA replication.

Lysine methylation in a histone protein produces a form of chromatin known as with the genes being transcriptionally __.