Question 1

What is required in order to study a protein's fine structure or function?

Accepted Answer

A) It must be isolated in a relatively pure state. 
B) It must be observed in a light microscope. 
C) It must be denatured. 
D) It must be enzymatically degraded. 
E) It must be fixed. 
A) It must be isolated in a relatively pure state. 
B) It must be observed in a light microscope. 
C) It must be denatured. 
D) It must be enzymatically degraded. 
E) It must be fixed.

Question 2

If two DNA samples are exactly the same,what would you expect to happen if you exposed both to the same mixture of primers and then carried out the polymerase chain reaction (PCR)?

Accepted Answer

A) Nothing at all happens. 
B) The PCR products should be exactly the same. 
C) There should be differences in the nature of PCR products. 
D) All PCR products in one of the samples will be destroyed. 
E) All PCR products in one of the samples will be methylated. 
A) Nothing at all happens. 
B) The PCR products should be exactly the same. 
C) There should be differences in the nature of PCR products. 
D) All PCR products in one of the samples will be destroyed. 
E) All PCR products in one of the samples will be methylated.

Question 3

What is a chelator?

Accepted Answer

A) a substance that precipitates cells 
B) a substance that degrades the proteins that hold cells together 
C) a substance that binds to or chelates calcium ions to remove them from solution 
D) a substance that precipitates calcium ions 
E) a substance that adds calcium ions to a solution 
A) a substance that precipitates cells 
B) a substance that degrades the proteins that hold cells together 
C) a substance that binds to or chelates calcium ions to remove them from solution 
D) a substance that precipitates calcium ions 
E) a substance that adds calcium ions to a solution

Question 4

How can a researcher visualize all of the DNA fragments present in an electrophoresis gel?

Accepted Answer

A) using a labeled probe with a sequence complementary to the desired DNA fragment 
B) staining with ethidium bromide 
C) staining with potassium chloride 
D) using labeled antibodies 
E) staining with Coomassie Blue 
A) using a labeled probe with a sequence complementary to the desired DNA fragment 
B) staining with ethidium bromide 
C) staining with potassium chloride 
D) using labeled antibodies 
E) staining with Coomassie Blue

Question 5

Which of the two earliest sequencing methodologies used a chemical approach?

Accepted Answer

A) the Sanger-Coulson method 
B) the Maxam-Gilbert method 
C) the cycle sequencing method 
D) the Sanger-Gilbert method 
E) the Maxam-Coulson method 
A) the Sanger-Coulson method 
B) the Maxam-Gilbert method 
C) the cycle sequencing method 
D) the Sanger-Gilbert method 
E) the Maxam-Coulson method

Question 6

A(n)_________ is responsible for collecting light rays previously focused on the specimen.

Accepted Answer

A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob 
A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob

Question 7

What is usually the first step in the purification of DNA?

Accepted Answer

A) homogenization of cells and isolation of nuclei 
B) fixation of the cells 
C) isolation of mitochondria and chloroplasts 
D) crystallization of DNA 
E) removal of deoxyribonucleases 
A) homogenization of cells and isolation of nuclei 
B) fixation of the cells 
C) isolation of mitochondria and chloroplasts 
D) crystallization of DNA 
E) removal of deoxyribonucleases

Question 8

The fluorescence imaging method in which a specimen containing one or more photoswitchable fluorophores is illuminated with pulses of light is known as:

Accepted Answer

A) Fluorescence recovery after photobleaching 
B) Laser scanning confocal microscopy 
C) Super-resolution microscopy 
D) Light sheet microscopy 
E) Fluorescence resonance energy transfer 
A) Fluorescence recovery after photobleaching 
B) Laser scanning confocal microscopy 
C) Super-resolution microscopy 
D) Light sheet microscopy 
E) Fluorescence resonance energy transfer

Question 9

What enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

Accepted Answer

A) RNase H 
B) reverse transcriptase 
C) DNA polymerase I 
D) RNase K 
E) DNA ligase 
A) RNase H 
B) reverse transcriptase 
C) DNA polymerase I 
D) RNase K 
E) DNA ligase

Question 10

The electron beam strikes the specimen in an area where there is a high concentration of heavy metal atoms.What does the spot on the phosphorescent screen of the electron microscope just below this part of the specimen look like?

Accepted Answer

A) It is dark. 
B) It is bright. 
C) It is speckled. 
D) It is invisible. 
E) It is purple. 
A) It is dark. 
B) It is bright. 
C) It is speckled. 
D) It is invisible. 
E) It is purple.

Question 11

Which technique could be used for molecular weight (mass)determinations in proteins?

Accepted Answer

A) X- ray diffraction 
B) chromatofocusing 
C) diethylaminoethyl (DEA
E) cellulose chromatography 
D) affinity chromatography 
E) SDS-PAGE 
A) X- ray diffraction 
B) chromatofocusing 
C) diethylaminoethyl (DEA
E) cellulose chromatography 
D) affinity chromatography 
E) SDS-PAGE

Question 12

As solvent passes through a column,it is collected as it drips out of the bottom of the column into a series of tubes; each of these tubes is called a(n)____________.

Accepted Answer

A) elutant 
B) fractal 
C) diluent 
D) fraction 
E) comutant 
A) elutant 
B) fractal 
C) diluent 
D) fraction 
E) comutant

Question 13

What advantage do images produced by freeze-fracturing and freeze-etching have over images generated with freeze-fracturing alone?

Accepted Answer

A) The images with freeze-fracture-freeze-etch are three-dimensional. 
B) The images with freeze-fracture-freeze-etch are in color. 
C) The images with freeze-fracture-freeze-etch reveal both the external and the internal surfaces of the cell membrane. 
D) The images with freeze-fracture-freeze-etch reveal only the external surface of the cell membrane. 
E) The images with freeze-fracture-freeze-etch reveal only the internal surface of the cell membrane. 
A) The images with freeze-fracture-freeze-etch are three-dimensional. 
B) The images with freeze-fracture-freeze-etch are in color. 
C) The images with freeze-fracture-freeze-etch reveal both the external and the internal surfaces of the cell membrane. 
D) The images with freeze-fracture-freeze-etch reveal only the external surface of the cell membrane. 
E) The images with freeze-fracture-freeze-etch reveal only the internal surface of the cell membrane.

Question 14

Why does the S value alone not provide a measure of molecular mass?

Accepted Answer

A) It is too difficult to measure. 
B) The speed with which a particle travels through a liquid column is not uniform. 
C) The velocity at which a particle moves through a liquid column depends on a number of factors,including shape. 
D) The velocity of movement accelerates as the particle approaches the bottom of the tube. 
E) The velocity of movement slows up as the particle approaches the top of the tube. 
A) It is too difficult to measure. 
B) The speed with which a particle travels through a liquid column is not uniform. 
C) The velocity at which a particle moves through a liquid column depends on a number of factors,including shape. 
D) The velocity of movement accelerates as the particle approaches the bottom of the tube. 
E) The velocity of movement slows up as the particle approaches the top of the tube.

Question 15

After plant cells have been separated and grown in culture,they can grow into an undifferentiated clump of cells called a(n)__________.

Accepted Answer

A) callus 
B) callous 
C) blastema 
D) tumor 
E) blastula 
A) callus 
B) callous 
C) blastema 
D) tumor 
E) blastula

Question 16

PCR employs a heat-stable DNA polymerase called ________,originally isolated from a bacterium that lives in hot springs at temperatures higher than 90°C.

Accepted Answer

A) Tack polymerase 
B) DNA polymerase I 
C) reverse transcriptase 
D) Taq polymerase 
E) poly(
A) polymerase 
A) Tack polymerase 
B) DNA polymerase I 
C) reverse transcriptase 
D) Taq polymerase 
E) poly(
A) polymerase

Question 17

The fluorescence imaging method in which an excitation laser is focused into a thin,flat sheet that illuminates the sample along a plane perpendicular to the angle of observation is known as:

Accepted Answer

A) Fluorescence recovery after photobleaching 
B) Laser scanning confocal microscopy 
C) Super-resolution microscopy 
D) Light sheet microscopy 
E) Fluorescence resonance energy transfer 
A) Fluorescence recovery after photobleaching 
B) Laser scanning confocal microscopy 
C) Super-resolution microscopy 
D) Light sheet microscopy 
E) Fluorescence resonance energy transfer

Question 18

__________ is the ratio of the amount of the protein of interest to the total amount of total protein present in the sample.

Accepted Answer

A) Radioactivity 
B) Special activity 
C) Specific activity 
D) Magnetic activity 
E) Proteinaceous activity 
A) Radioactivity 
B) Special activity 
C) Specific activity 
D) Magnetic activity 
E) Proteinaceous activity

Question 19

What method is typically used to dry specimens for scanning electron microscopy?

Accepted Answer

A) air drying 
B) critical-point drying 
C) forced air drying 
D) vacuum drying 
E) dehydrational drying 
A) air drying 
B) critical-point drying 
C) forced air drying 
D) vacuum drying 
E) dehydrational drying

Question 20

Why do electron microscopes provide much greater resolving power than light microscopes?

Accepted Answer

A) because more electrical power is involved in running an electron microscope 
B) because the wavelength of an electron is much lower than that of visible light 
C) because the wavelength of an electron is much higher than that of visible light 
D) because the numerical aperture is much larger in electron microscopes 
E) because the numerical aperture is much smaller in electron microscopes 
A) because more electrical power is involved in running an electron microscope 
B) because the wavelength of an electron is much lower than that of visible light 
C) because the wavelength of an electron is much higher than that of visible light 
D) because the numerical aperture is much larger in electron microscopes 
E) because the numerical aperture is much smaller in electron microscopes

What is required in order to study a protein's fine structure or function?

If two DNA samples are exactly the same,what would you expect to happen if you exposed both to the same mixture of primers and then carried out the polymerase chain reaction (PCR)?

What is a chelator?

How can a researcher visualize all of the DNA fragments present in an electrophoresis gel?

Which of the two earliest sequencing methodologies used a chemical approach?

A(n)_________ is responsible for collecting light rays previously focused on the specimen.

What is usually the first step in the purification of DNA?

The fluorescence imaging method in which a specimen containing one or more photoswitchable fluorophores is illuminated with pulses of light is known as:

What enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

The electron beam strikes the specimen in an area where there is a high concentration of heavy metal atoms.What does the spot on the phosphorescent screen of the electron microscope just below this part of the specimen look like?

Which technique could be used for molecular weight (mass)determinations in proteins?

As solvent passes through a column,it is collected as it drips out of the bottom of the column into a series of tubes; each of these tubes is called a(n)____________.

What advantage do images produced by freeze-fracturing and freeze-etching have over images generated with freeze-fracturing alone?

Why does the S value alone not provide a measure of molecular mass?

After plant cells have been separated and grown in culture,they can grow into an undifferentiated clump of cells called a(n)__________.

PCR employs a heat-stable DNA polymerase called ________,originally isolated from a bacterium that lives in hot springs at temperatures higher than 90°C.

The fluorescence imaging method in which an excitation laser is focused into a thin,flat sheet that illuminates the sample along a plane perpendicular to the angle of observation is known as:

__________ is the ratio of the amount of the protein of interest to the total amount of total protein present in the sample.

What method is typically used to dry specimens for scanning electron microscopy?

Why do electron microscopes provide much greater resolving power than light microscopes?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics,enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Exam 18: Techniques in Cell and Molecular Biology

What is required in order to study a protein's fine structure or function?

If two DNA samples are exactly the same,what would you expect to happen if you exposed both to the same mixture of primers and then carried out the polymerase chain reaction (PCR)?

What is a chelator?

How can a researcher visualize all of the DNA fragments present in an electrophoresis gel?

Which of the two earliest sequencing methodologies used a chemical approach?

A(n)_________ is responsible for collecting light rays previously focused on the specimen.

What is usually the first step in the purification of DNA?

The fluorescence imaging method in which a specimen containing one or more photoswitchable fluorophores is illuminated with pulses of light is known as:

What enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

The electron beam strikes the specimen in an area where there is a high concentration of heavy metal atoms.What does the spot on the phosphorescent screen of the electron microscope just below this part of the specimen look like?

Which technique could be used for molecular weight (mass)determinations in proteins?

As solvent passes through a column,it is collected as it drips out of the bottom of the column into a series of tubes; each of these tubes is called a(n)____________.

What advantage do images produced by freeze-fracturing and freeze-etching have over images generated with freeze-fracturing alone?

Why does the S value alone not provide a measure of molecular mass?

After plant cells have been separated and grown in culture,they can grow into an undifferentiated clump of cells called a(n)__________.

PCR employs a heat-stable DNA polymerase called ________,originally isolated from a bacterium that lives in hot springs at temperatures higher than 90°C.

The fluorescence imaging method in which an excitation laser is focused into a thin,flat sheet that illuminates the sample along a plane perpendicular to the angle of observation is known as:

__________ is the ratio of the amount of the protein of interest to the total amount of total protein present in the sample.

What method is typically used to dry specimens for scanning electron microscopy?

Why do electron microscopes provide much greater resolving power than light microscopes?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics,enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters