Question 1

What determines the identity and chemical properties of an atom?

Accepted Answer

A) the number of protons in its nucleus 
B) the number of neutrons in its nucleus 
C) the number of electrons in its nucleus 
D) the number of protons in the atom's electron shells 
E) the number of neutrons in the atom's electron shells 
A) the number of protons in its nucleus 
B) the number of neutrons in its nucleus 
C) the number of electrons in its nucleus 
D) the number of protons in the atom's electron shells 
E) the number of neutrons in the atom's electron shells

Question 2

Why have most clinical trials in which retroviruses have been used to introduce genes for the purpose of gene therapy in patients lacking that gene been unsuccessful?

Accepted Answer

A) Viral DNA is more unstable than mammalian DNA. 
B) Current viral vectors have too high an infection efficiency rate. 
C) Current viral vectors have too low an infection efficiency rate. 
D) Mammalian DNA is more unstable than viral DNA. 
E) Current viral vectors tend to cause serious diseases. 
A) Viral DNA is more unstable than mammalian DNA. 
B) Current viral vectors have too high an infection efficiency rate. 
C) Current viral vectors have too low an infection efficiency rate. 
D) Mammalian DNA is more unstable than viral DNA. 
E) Current viral vectors tend to cause serious diseases.

Question 3

What presently supplies reduced nitrogen compounds to crop plants that are unable to fix nitrogen from the atmosphere on their own?

Accepted Answer

A) phosphates 
B) water 
C) fertilizers 
D) soil 
E) sunlight 
A) phosphates 
B) water 
C) fertilizers 
D) soil 
E) sunlight

Question 4

An antibody molecule preparation made by a single colony or clone of antibody-producing cells would consist of large quantities of antibodies that all had exactly the same antigen-combining site.Such antibodies would be called _________ antibodies.

Accepted Answer

A) polyclonal 
B) monoclonal 
C) polyvalent 
D) monotreme 
E) monocular 
A) polyclonal 
B) monoclonal 
C) polyvalent 
D) monotreme 
E) monocular

Question 5

Cells that are capable of unlimited division are said to be _________.

Accepted Answer

A) mortal 
B) lifeless 
C) immortal 
D) perpetual 
E) preserved 
A) mortal 
B) lifeless 
C) immortal 
D) perpetual 
E) preserved

Question 6

Which of the following words is a correct description of image formation in the scanning electron microscope?

Accepted Answer

A) direct 
B) explosive 
C) indirect 
D) flexible 
E) inflexible 
A) direct 
B) explosive 
C) indirect 
D) flexible 
E) inflexible

Question 7

Nucleic acid hybridization techniques are based on the observation that ______________.

Accepted Answer

A) DNA can be very long 
B) two single-stranded nucleic acid molecules of complementary base sequence can form a double-stranded hybrid 
C) DNA molecules are very hydrophobic. 
D) two single-stranded nucleic acid molecules of any base sequence can form a double-stranded hybrid 
E) two double-stranded nucleic acid molecules of complementary base sequence can form a double-stranded hybrid 
A) DNA can be very long 
B) two single-stranded nucleic acid molecules of complementary base sequence can form a double-stranded hybrid 
C) DNA molecules are very hydrophobic. 
D) two single-stranded nucleic acid molecules of any base sequence can form a double-stranded hybrid 
E) two double-stranded nucleic acid molecules of complementary base sequence can form a double-stranded hybrid

Question 8

Which of the following is not a commonly used fluorophore?

Accepted Answer

A) GFP 
B) CFP 
C) EPuce 
D) EYFP 
E) mBanana 
A) GFP 
B) CFP 
C) EPuce 
D) EYFP 
E) mBanana

Question 9

Which electron microscope technique below is used to view the surfaces of objects ranging in size from a virus to an animal's head and generates a three-dimensional image?

Accepted Answer

A) positive staining 
B) negative staining 
C) scanning electron microscopy 
D) shadow casting 
E) freeze-fracture freeze-etch 
A) positive staining 
B) negative staining 
C) scanning electron microscopy 
D) shadow casting 
E) freeze-fracture freeze-etch

Question 10

A permanent collection of all of the sequences in the genome of a species is called a(n)_____.

Accepted Answer

A) library 
B) biotome 
C) libotome 
D) acetome 
E) topiary 
A) library 
B) biotome 
C) libotome 
D) acetome 
E) topiary

Question 11

Microinjection is a technique in which ____________.

Accepted Answer

A) mRNAs are injected directly into the cell nucleus. 
B) DNA is injected directly into the cell nucleus. 
C) DNA is injected directly into the mitochondria. 
D) DNA is injected directly into the cell cytoplasm. 
E) DNA is injected directly into phagosomes. 
A) mRNAs are injected directly into the cell nucleus. 
B) DNA is injected directly into the cell nucleus. 
C) DNA is injected directly into the mitochondria. 
D) DNA is injected directly into the cell cytoplasm. 
E) DNA is injected directly into phagosomes.

Question 12

About how big are the DNA fragments that can be placed into phage $\lambda$ and cloned?

Accepted Answer

A) 15 kb in length 
B) 20 kb in length 
C) 50 kb in length 
D) 100 kb in length 
E) 10 kb in length 
A) 15 kb in length 
B) 20 kb in length 
C) 50 kb in length 
D) 100 kb in length 
E) 10 kb in length

Question 13

What device is used to automatically change the temperature of the PCR reaction mixture,allowing each step in the cycle to take place?

Accepted Answer

A) a water bath 
B) a thermal cycler 
C) an ice bucket 
D) a thermocoupler 
E) a thermometer 
A) a water bath 
B) a thermal cycler 
C) an ice bucket 
D) a thermocoupler 
E) a thermometer

Question 14

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA.The ends of the altered DNA are then ligated back together.What is the effect on the gene sequence?

Accepted Answer

A) Its amino acids are altered. 
B) The reading frame of the gene is destroyed. 
C) The gene is unchanged. 
D) The gene is transposed. 
E) The gene is inverted. 
A) Its amino acids are altered. 
B) The reading frame of the gene is destroyed. 
C) The gene is unchanged. 
D) The gene is transposed. 
E) The gene is inverted.

Question 15

What typically happens if the cells of a cell line are injected into susceptible lab animals?

Accepted Answer

A) They become a normal part of the appropriate tissue in the lab animal. 
B) They soon die. 
C) They typically grow into malignant tumors. 
D) They typically grow into the appropriate organ. 
E) They become part of organs 
A) They become a normal part of the appropriate tissue in the lab animal. 
B) They soon die. 
C) They typically grow into malignant tumors. 
D) They typically grow into the appropriate organ. 
E) They become part of organs

Question 16

What is the process of transferring single-stranded DNA from a gel to a nitrocellulose membrane called?

Accepted Answer

A) transferral 
B) blotting 
C) transdetermination 
D) besotting 
E) allotting 
A) transferral 
B) blotting 
C) transdetermination 
D) besotting 
E) allotting

Question 17

Why do phenol and buffered saline separate into two phases when one centrifuges the mixture during the DNA extraction procedure?

Accepted Answer

A) They are soluble. 
B) They are miscible. 
C) They are immiscible. 
D) One freezes and the other does not. 
E) They are both hydrophobic. 
A) They are soluble. 
B) They are miscible. 
C) They are immiscible. 
D) One freezes and the other does not. 
E) They are both hydrophobic.

Question 18

Two fluorescent proteins (Protein 1 and Protein 2)are used in a FRET experiment and are illuminated with light that excites Protein 1.If the proteins are close enough for energy transfer to occur,what wavelength(s)of light will be detected?

Accepted Answer

A) The light that excites Protein 1 
B) The light that is emitted by Protein 1 
C) The light that excites Protein 2 
D) The light that is emitted by Protein 2 
E) No light will be detected,because the fluorescence of Proteins 1 and 2 will cancel out 
A) The light that excites Protein 1 
B) The light that is emitted by Protein 1 
C) The light that excites Protein 2 
D) The light that is emitted by Protein 2 
E) No light will be detected,because the fluorescence of Proteins 1 and 2 will cancel out

Question 19

What is the term for a variety of techniques in which a mixture of dissolved components is fractionated as it moves through a porous matrix?

Accepted Answer

A) electrophoresis 
B) isoelectric focusing 
C) chromatography 
D) selective precipitation 
E) sucrose density centrifugation 
A) electrophoresis 
B) isoelectric focusing 
C) chromatography 
D) selective precipitation 
E) sucrose density centrifugation

Question 20

When making a measurement in a spectrophotometer if you are illuminating a specimen with ultraviolet light,into what kind of container should it be placed?

Accepted Answer

A) in a basinette 
B) in a special,flat-sided quartz container 
C) in a borosilicate glass tube 
D) in a special,flat-sided glass container 
E) in a special,flat-sided plastic container 
A) in a basinette 
B) in a special,flat-sided quartz container 
C) in a borosilicate glass tube 
D) in a special,flat-sided glass container 
E) in a special,flat-sided plastic container

What determines the identity and chemical properties of an atom?

Why have most clinical trials in which retroviruses have been used to introduce genes for the purpose of gene therapy in patients lacking that gene been unsuccessful?

What presently supplies reduced nitrogen compounds to crop plants that are unable to fix nitrogen from the atmosphere on their own?

An antibody molecule preparation made by a single colony or clone of antibody-producing cells would consist of large quantities of antibodies that all had exactly the same antigen-combining site.Such antibodies would be called _________ antibodies.

Cells that are capable of unlimited division are said to be _________.

Which of the following words is a correct description of image formation in the scanning electron microscope?

Nucleic acid hybridization techniques are based on the observation that ______________.

Which of the following is not a commonly used fluorophore?

Which electron microscope technique below is used to view the surfaces of objects ranging in size from a virus to an animal's head and generates a three-dimensional image?

A permanent collection of all of the sequences in the genome of a species is called a(n)_____.

Microinjection is a technique in which ____________.

About how big are the DNA fragments that can be placed into phage $\lambda$ and cloned?

What device is used to automatically change the temperature of the PCR reaction mixture,allowing each step in the cycle to take place?

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA.The ends of the altered DNA are then ligated back together.What is the effect on the gene sequence?

What typically happens if the cells of a cell line are injected into susceptible lab animals?

What is the process of transferring single-stranded DNA from a gel to a nitrocellulose membrane called?

Why do phenol and buffered saline separate into two phases when one centrifuges the mixture during the DNA extraction procedure?

Two fluorescent proteins (Protein 1 and Protein 2)are used in a FRET experiment and are illuminated with light that excites Protein 1.If the proteins are close enough for energy transfer to occur,what wavelength(s)of light will be detected?

What is the term for a variety of techniques in which a mixture of dissolved components is fractionated as it moves through a porous matrix?

When making a measurement in a spectrophotometer if you are illuminating a specimen with ultraviolet light,into what kind of container should it be placed?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics,enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Exam 18: Techniques in Cell and Molecular Biology

What determines the identity and chemical properties of an atom?

Why have most clinical trials in which retroviruses have been used to introduce genes for the purpose of gene therapy in patients lacking that gene been unsuccessful?

What presently supplies reduced nitrogen compounds to crop plants that are unable to fix nitrogen from the atmosphere on their own?

An antibody molecule preparation made by a single colony or clone of antibody-producing cells would consist of large quantities of antibodies that all had exactly the same antigen-combining site.Such antibodies would be called _________ antibodies.

Cells that are capable of unlimited division are said to be _________.

Which of the following words is a correct description of image formation in the scanning electron microscope?

Nucleic acid hybridization techniques are based on the observation that ______________.

Which of the following is not a commonly used fluorophore?

Which electron microscope technique below is used to view the surfaces of objects ranging in size from a virus to an animal's head and generates a three-dimensional image?

A permanent collection of all of the sequences in the genome of a species is called a(n)_____.

Microinjection is a technique in which ____________.

About how big are the DNA fragments that can be placed into phage λ\lambdaλ and cloned?

What device is used to automatically change the temperature of the PCR reaction mixture,allowing each step in the cycle to take place?

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA.The ends of the altered DNA are then ligated back together.What is the effect on the gene sequence?

What typically happens if the cells of a cell line are injected into susceptible lab animals?

What is the process of transferring single-stranded DNA from a gel to a nitrocellulose membrane called?

Why do phenol and buffered saline separate into two phases when one centrifuges the mixture during the DNA extraction procedure?

Two fluorescent proteins (Protein 1 and Protein 2)are used in a FRET experiment and are illuminated with light that excites Protein 1.If the proteins are close enough for energy transfer to occur,what wavelength(s)of light will be detected?

What is the term for a variety of techniques in which a mixture of dissolved components is fractionated as it moves through a porous matrix?

When making a measurement in a spectrophotometer if you are illuminating a specimen with ultraviolet light,into what kind of container should it be placed?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics,enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

About how big are the DNA fragments that can be placed into phage $\lambda$ and cloned?