Question 1

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

Accepted Answer

A) if it has a greater density than the surrounding medium 
B) if it has a lower density than the surrounding medium 
C) if it is wet 
D) if the surrounding medium is denser than the component 
E) if the component and the surrounding medium have equal density 
A) if it has a greater density than the surrounding medium 
B) if it has a lower density than the surrounding medium 
C) if it is wet 
D) if the surrounding medium is denser than the component 
E) if the component and the surrounding medium have equal density A

Question 2

Under what set of circumstances will organelles move to the bottom of a centrifuge tube in a centrifugal field?

Accepted Answer

A) if the organelle is smaller than ribosomes 
B) if the organelle has too much fat content 
C) if the organelle is less dense than the surrounding medium 
D) if the organelle is more dense than the surrounding medium 
E) if the organelle has less fat content than the surrounding medium 
A) if the organelle is smaller than ribosomes 
B) if the organelle has too much fat content 
C) if the organelle is less dense than the surrounding medium 
D) if the organelle is more dense than the surrounding medium 
E) if the organelle has less fat content than the surrounding medium D

Question 3

Which part of a microscope uses the real,enlarged image formed by the objective lens in the column of the microscope and forms from it an enlarged,virtual image?

Accepted Answer

A) condenser lens 
B) the numerical aperture 
C) ocular lens 
D) iris 
E) focusing knob 
A) condenser lens 
B) the numerical aperture 
C) ocular lens 
D) iris 
E) focusing knob C

Question 4

What enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

Accepted Answer

A) RNase H 
B) reverse transcriptase 
C) DNA polymerase I 
D) RNase K 
E) DNA ligase 
A) RNase H 
B) reverse transcriptase 
C) DNA polymerase I 
D) RNase K 
E) DNA ligase

Question 5

The electron beam strikes the specimen in an area where there is a high concentration of heavy metal atoms.What does the spot on the phosphorescent screen of the electron microscope just below this part of the specimen look like?

Accepted Answer

A) It is dark. 
B) It is bright. 
C) It is speckled. 
D) It is invisible. 
E) It is purple. 
A) It is dark. 
B) It is bright. 
C) It is speckled. 
D) It is invisible. 
E) It is purple.

Question 6

A(n)________ is a substance that reveals its presence in one way or another and thus can be localized or monitored during an experiment.

Accepted Answer

A) trancer 
B) tracer 
C) phosphor 
D) fluor 
E) trancept 
A) trancer 
B) tracer 
C) phosphor 
D) fluor 
E) trancept

Question 7

Why does centrifugation at high speeds proceed in a near vacuum?

Accepted Answer

A) to maximize frictional resistance to the rotor's motion 
B) to minimize frictional resistance to the rotor's motion 
C) to balance the rotor 
D) to stabilize the rotor 
E) to minimize pH fluctuations 
A) to maximize frictional resistance to the rotor's motion 
B) to minimize frictional resistance to the rotor's motion 
C) to balance the rotor 
D) to stabilize the rotor 
E) to minimize pH fluctuations

Question 8

Which form of radiation is equivalent to an electron?

Accepted Answer

A) an alpha particle 
B) a delta particle 
C) a beta particle 
D) gamma radiation 
E) an omega particle 
A) an alpha particle 
B) a delta particle 
C) a beta particle 
D) gamma radiation 
E) an omega particle

Question 9

The first fully human antibody has been manufactured using bacteriophage rather than hybridomas;it is approved for rheumatoid arthritis treatment and is called _________.

Accepted Answer

A) Phagoma 
B) Humira 
C) Humana 
D) Humantibody 
E) Amana 
A) Phagoma 
B) Humira 
C) Humana 
D) Humantibody 
E) Amana

Question 10

Which technique for detecting radioactivity is the best one for quantifying the amount of radioactivity in a specimen?

Accepted Answer

A) autoradiography 
B) an NMR machine 
C) liquid scintillation spectrometry 
D) mass spectrometry 
E) AFM 
A) autoradiography 
B) an NMR machine 
C) liquid scintillation spectrometry 
D) mass spectrometry 
E) AFM

Question 11

In what electrophoresis medium are small RNAs or DNAs of a few hundred nucleotides typically separated?

Accepted Answer

A) agarose 
B) polyacrylamide 
C) cellulose 
D) paper 
E) dextran 
A) agarose 
B) polyacrylamide 
C) cellulose 
D) paper 
E) dextran

Question 12

How are probes labeled with a radioactive isotope detected?

Accepted Answer

A) autoradiography 
B) fluorescence 
C) by changing colors 
D) SEM 
E) TEM 
A) autoradiography 
B) fluorescence 
C) by changing colors 
D) SEM 
E) TEM

Question 13

For each protein,there is a pH at which negative and positive charges are equal.This pH is referred to as the ___________.

Accepted Answer

A) pH point 
B) neutralization point 
C) isometric point 
D) isoelectric point 
E) isotonic point 
A) pH point 
B) neutralization point 
C) isometric point 
D) isoelectric point 
E) isotonic point

Question 14

Which of the following is a technique used to encourage bacteria to take up DNA from the surrounding culture medium?

Accepted Answer

A) pretreatment of bacteria with a brief heat shock,followed by exposure to Ca2+ ions 
B) pretreatment of bacteria with Cu2+ ions,followed by a brief heat shock 
C) pretreatment of bacteria with Ca2+ ions,followed by a brief heat shock 
D) pretreatment of bacteria with Ca2+ ions,followed by a brief cold shock 
E) pretreatment of bacteria with a brief heat shock,followed by exposure to Mg2+ ions 
A) pretreatment of bacteria with a brief heat shock,followed by exposure to Ca2+ ions 
B) pretreatment of bacteria with Cu2+ ions,followed by a brief heat shock 
C) pretreatment of bacteria with Ca2+ ions,followed by a brief heat shock 
D) pretreatment of bacteria with Ca2+ ions,followed by a brief cold shock 
E) pretreatment of bacteria with a brief heat shock,followed by exposure to Mg2+ ions

Question 15

When malignant myeloma cells and normal lymphocytes are fused together to make hybrid cells,the cells thus produced are called _________.

Accepted Answer

A) lymphomas 
B) antibomas 
C) hybridomas 
D) myelobrids 
E) lymphocytomas 
A) lymphomas 
B) antibomas 
C) hybridomas 
D) myelobrids 
E) lymphocytomas

Question 16

What is the name of a piece of DNA containing sequences derived from more than one organism?

Accepted Answer

A) combinational DNA 
B) recombinant DNA 
C) combined DNA 
D) cloned DNA 
E) recreational DNA 
A) combinational DNA 
B) recombinant DNA 
C) combined DNA 
D) cloned DNA 
E) recreational DNA

Question 17

What device is used to automatically change the temperature of the PCR reaction mixture,allowing each step in the cycle to take place?

Accepted Answer

A) a water bath 
B) a thermal cycler 
C) an ice bucket 
D) a thermocoupler 
E) a thermometer 
A) a water bath 
B) a thermal cycler 
C) an ice bucket 
D) a thermocoupler 
E) a thermometer

Question 18

Of what is the electron source of an electron microscope,the cathode,composed?

Accepted Answer

A) a tungsten wire filament 
B) an iron wire filament 
C) a titanium wire filament 
D) an iron spike filament 
E) a tungsten spike filament 
A) a tungsten wire filament 
B) an iron wire filament 
C) a titanium wire filament 
D) an iron spike filament 
E) a tungsten spike filament

Question 19

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

Accepted Answer

A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob 
A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob

Question 20

Why is a uniform layer of carbon applied to a replica after the metal has coated the specimen at an angle?

Accepted Answer

A) to make the replica smooth 
B) to cement the patches of metal into a solid surface 
C) to make the replica rough 
D) to make the replica transparent to electrons 
E) to make the replica opaque to electrons 
A) to make the replica smooth 
B) to cement the patches of metal into a solid surface 
C) to make the replica rough 
D) to make the replica transparent to electrons 
E) to make the replica opaque to electrons

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

Under what set of circumstances will organelles move to the bottom of a centrifuge tube in a centrifugal field?

Which part of a microscope uses the real,enlarged image formed by the objective lens in the column of the microscope and forms from it an enlarged,virtual image?

What enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

The electron beam strikes the specimen in an area where there is a high concentration of heavy metal atoms.What does the spot on the phosphorescent screen of the electron microscope just below this part of the specimen look like?

A(n)________ is a substance that reveals its presence in one way or another and thus can be localized or monitored during an experiment.

Why does centrifugation at high speeds proceed in a near vacuum?

Which form of radiation is equivalent to an electron?

The first fully human antibody has been manufactured using bacteriophage rather than hybridomas;it is approved for rheumatoid arthritis treatment and is called _________.

Which technique for detecting radioactivity is the best one for quantifying the amount of radioactivity in a specimen?

In what electrophoresis medium are small RNAs or DNAs of a few hundred nucleotides typically separated?

How are probes labeled with a radioactive isotope detected?

For each protein,there is a pH at which negative and positive charges are equal.This pH is referred to as the ___________.

Which of the following is a technique used to encourage bacteria to take up DNA from the surrounding culture medium?

When malignant myeloma cells and normal lymphocytes are fused together to make hybrid cells,the cells thus produced are called _________.

What is the name of a piece of DNA containing sequences derived from more than one organism?

What device is used to automatically change the temperature of the PCR reaction mixture,allowing each step in the cycle to take place?

Of what is the electron source of an electron microscope,the cathode,composed?

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

Why is a uniform layer of carbon applied to a replica after the metal has coated the specimen at an angle?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes, and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

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Exam 18: Techniques in Cell and Molecular Biology

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

Under what set of circumstances will organelles move to the bottom of a centrifuge tube in a centrifugal field?

Which part of a microscope uses the real,enlarged image formed by the objective lens in the column of the microscope and forms from it an enlarged,virtual image?

What enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?

The electron beam strikes the specimen in an area where there is a high concentration of heavy metal atoms.What does the spot on the phosphorescent screen of the electron microscope just below this part of the specimen look like?

A(n)________ is a substance that reveals its presence in one way or another and thus can be localized or monitored during an experiment.

Why does centrifugation at high speeds proceed in a near vacuum?

Which form of radiation is equivalent to an electron?

The first fully human antibody has been manufactured using bacteriophage rather than hybridomas;it is approved for rheumatoid arthritis treatment and is called _________.

Which technique for detecting radioactivity is the best one for quantifying the amount of radioactivity in a specimen?

In what electrophoresis medium are small RNAs or DNAs of a few hundred nucleotides typically separated?

How are probes labeled with a radioactive isotope detected?

For each protein,there is a pH at which negative and positive charges are equal.This pH is referred to as the ___________.

Which of the following is a technique used to encourage bacteria to take up DNA from the surrounding culture medium?

When malignant myeloma cells and normal lymphocytes are fused together to make hybrid cells,the cells thus produced are called _________.

What is the name of a piece of DNA containing sequences derived from more than one organism?

What device is used to automatically change the temperature of the PCR reaction mixture,allowing each step in the cycle to take place?

Of what is the electron source of an electron microscope,the cathode,composed?

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

Why is a uniform layer of carbon applied to a replica after the metal has coated the specimen at an angle?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes, and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters