Question 1

Antibodies can distinguish between two polypeptides that differ by as little as __________.

Accepted Answer

A) one amino acid 
B) two amino acids 
C) five amino acids 
D) one nucleotide 
E) two nucleotides 
A) one amino acid 
B) two amino acids 
C) five amino acids 
D) one nucleotide 
E) two nucleotides

Question 2

_________ are established with cells that have been frozen in liquid nitrogen,thawed and then placed in a waiting culture medium.They are usually previously cultured cells that were frozen for storage.

Accepted Answer

A) Primary cultures 
B) Secondary cultures 
C) Tertiary cultures 
D) Supernatant cultures 
E) Penultimate cultures 
A) Primary cultures 
B) Secondary cultures 
C) Tertiary cultures 
D) Supernatant cultures 
E) Penultimate cultures

Question 3

How big are the sites recognized by most restriction enzymes?

Accepted Answer

A) 4 - 6 nucleotides 
B) 6 - 8 nucleotides 
C) 2 - 4 nucleotides 
D) 6 - 10 nucleotides 
E) 2 - 10 nucleotides 
A) 4 - 6 nucleotides 
B) 6 - 8 nucleotides 
C) 2 - 4 nucleotides 
D) 6 - 10 nucleotides 
E) 2 - 10 nucleotides

Question 4

What is used to separate DNA fragments in the cycle sequencing method of DNA sequencing in such a way that chains differing by only one nucleotide in length can be separated?

Accepted Answer

A) SDS-PAGE 
B) non-denaturing PAGE 
C) agarose gel electrophoresis 
D) electrophoresis on very thin capillary gels 
E) gel filtration chromatography 
A) SDS-PAGE 
B) non-denaturing PAGE 
C) agarose gel electrophoresis 
D) electrophoresis on very thin capillary gels 
E) gel filtration chromatography

Question 5

If two DNA samples are exactly the same,what would you expect to happen if you exposed both to the same mixture of primers and then carried out the polymerase chain reaction (PCR)?

Accepted Answer

A) Nothing at all happens. 
B) The PCR products should be exactly the same. 
C) There should be differences in the nature of PCR products. 
D) All PCR products in one of the samples will be destroyed. 
E) All PCR products in one of the samples will be methylated. 
A) Nothing at all happens. 
B) The PCR products should be exactly the same. 
C) There should be differences in the nature of PCR products. 
D) All PCR products in one of the samples will be destroyed. 
E) All PCR products in one of the samples will be methylated.

Question 6

Which of the following is most likely to elute first from a gel filtration column if each of them is essentially globular?

Accepted Answer

A) a 250 kilodalton protein 
B) a 120 kilodalton protein 
C) a chromate ion 
D) a 12 kilodalton protein 
E) an 80 kilodalton protein 
A) a 250 kilodalton protein 
B) a 120 kilodalton protein 
C) a chromate ion 
D) a 12 kilodalton protein 
E) an 80 kilodalton protein

Question 7

A(n)_________ is responsible for collecting light rays previously focused on the specimen.

Accepted Answer

A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob 
A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob

Question 8

In which technique is radioactivity detected by mixing the sample in a vial with a special fluid that contains compounds that emit light if struck by a beta particle? The amount of light emitted in the fluid is a measure of the amount of radioactivity in sample.

Accepted Answer

A) autoradiography 
B) an NMR machine 
C) liquid scintillation spectrometry 
D) mass spectrometry 
E) AFM 
A) autoradiography 
B) an NMR machine 
C) liquid scintillation spectrometry 
D) mass spectrometry 
E) AFM

Question 9

What determines the identity and chemical properties of an atom?

Accepted Answer

A) the number of protons in its nucleus 
B) the number of neutrons in its nucleus 
C) the number of electrons in its nucleus 
D) the number of protons in the atom's electron shells 
E) the number of neutrons in the atom's electron shells 
A) the number of protons in its nucleus 
B) the number of neutrons in its nucleus 
C) the number of electrons in its nucleus 
D) the number of protons in the atom's electron shells 
E) the number of neutrons in the atom's electron shells

Question 10

What salt is most often used in selective precipitation experiments?

Accepted Answer

A) sodium chloride 
B) sodium carbonate 
C) ammonium sulfate 
D) ammonium chloride 
E) sodium thiocyanate 
A) sodium chloride 
B) sodium carbonate 
C) ammonium sulfate 
D) ammonium chloride 
E) sodium thiocyanate

Question 11

Another name for short,single-stranded nucleic acid molecules is _________.

Accepted Answer

A) mininucleotides 
B) micronucleotides 
C) polynucleotides 
D) oligonucleotides 
E) olivonucleotides 
A) mininucleotides 
B) micronucleotides 
C) polynucleotides 
D) oligonucleotides 
E) olivonucleotides

Question 12

Why does ion exchange chromatography depend on the pH of the medium?

Accepted Answer

A) The charge of each amino acid R group depends on the medium's pH. 
B) The charge of each nucleic acid R group depends on the medium's pH. 
C) The charge of each fatty acid R group depends on the medium's pH. 
D) The charge of each terminal amino acid depends on the medium's pH. 
E) The shape of each protein depends on the medium's pH. 
A) The charge of each amino acid R group depends on the medium's pH. 
B) The charge of each nucleic acid R group depends on the medium's pH. 
C) The charge of each fatty acid R group depends on the medium's pH. 
D) The charge of each terminal amino acid depends on the medium's pH. 
E) The shape of each protein depends on the medium's pH.

Question 13

The fractured surface of the specimen is coated with a heavy metal layer with the metal being deposited at an angle.A uniform carbon layer is deposited on top of the metal layer from directly overhead to cement the patches of metal into a solid layer.What is the name for this process?

Accepted Answer

A) freeze-fracture 
B) replication 
C) entrapment 
D) metallification 
E) freeze-etch 
A) freeze-fracture 
B) replication 
C) entrapment 
D) metallification 
E) freeze-etch

Question 14

What is the name of the process by which recombinant DNA gets into bacteria after being picked up from the surrounding medium?

Accepted Answer

A) transduction 
B) transmutation 
C) transformation 
D) transjunction 
E) transition 
A) transduction 
B) transmutation 
C) transformation 
D) transjunction 
E) transition

Question 15

Non-denaturing gel electrophoresis (PAGE)has the disadvantage that it ____________.

Accepted Answer

A) separates solely on the basis of charge 
B) allows the detection of proteins in a gel by their biological activity 
C) separates on the basis of more than one property thus to some degree confusing the results 
D) separates solely on the basis of molecular weight 
E) separates on the basis of molecular weight and frictional coefficient 
A) separates solely on the basis of charge 
B) allows the detection of proteins in a gel by their biological activity 
C) separates on the basis of more than one property thus to some degree confusing the results 
D) separates solely on the basis of molecular weight 
E) separates on the basis of molecular weight and frictional coefficient

Question 16

What is defined as viral-mediated gene transfer,which is a very widely used strategy to get genes into eukaryotic cells?

Accepted Answer

A) transduction 
B) transfection 
C) electroporation 
D) lipofection 
E) transgenics 
A) transduction 
B) transfection 
C) electroporation 
D) lipofection 
E) transgenics

Question 17

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA.The ends of the altered DNA are then ligated back together.What is the effect on the gene sequence?

Accepted Answer

A) Its amino acids are altered. 
B) The reading frame of the gene is destroyed. 
C) The gene is unchanged. 
D) The gene is transposed. 
E) The gene is inverted. 
A) Its amino acids are altered. 
B) The reading frame of the gene is destroyed. 
C) The gene is unchanged. 
D) The gene is transposed. 
E) The gene is inverted.

Question 18

What typically happens if the cells of a cell line are injected into susceptible lab animals?

Accepted Answer

A) They become a normal part of the appropriate tissue in the lab animal. 
B) They soon die. 
C) They typically grow into malignant tumors. 
D) They typically grow into the appropriate organ. 
E) They become part of organs 
A) They become a normal part of the appropriate tissue in the lab animal. 
B) They soon die. 
C) They typically grow into malignant tumors. 
D) They typically grow into the appropriate organ. 
E) They become part of organs

Question 19

The 3-D computerized reconstruction of a cell generated by cryoelectron tomography is called a(n)________.

Accepted Answer

A) tomatogram 
B) chromatogram 
C) tomogram 
D) telegram 
E) tonogram 
A) tomatogram 
B) chromatogram 
C) tomogram 
D) telegram 
E) tonogram

Question 20

Nucleic acid hybridization techniques are based on the observation that ______________.

Accepted Answer

A) DNA can be very long 
B) two single-stranded nucleic acid molecules of complementary base sequence can form a double-stranded hybrid 
C) DNA molecules are very hydrophobic. 
D) two single-stranded nucleic acid molecules of any base sequence can form a double-stranded hybrid 
E) two double-stranded nucleic acid molecules of complementary base sequence can form a double-stranded hybrid 
A) DNA can be very long 
B) two single-stranded nucleic acid molecules of complementary base sequence can form a double-stranded hybrid 
C) DNA molecules are very hydrophobic. 
D) two single-stranded nucleic acid molecules of any base sequence can form a double-stranded hybrid 
E) two double-stranded nucleic acid molecules of complementary base sequence can form a double-stranded hybrid

Antibodies can distinguish between two polypeptides that differ by as little as __________.

_________ are established with cells that have been frozen in liquid nitrogen,thawed and then placed in a waiting culture medium.They are usually previously cultured cells that were frozen for storage.

How big are the sites recognized by most restriction enzymes?

What is used to separate DNA fragments in the cycle sequencing method of DNA sequencing in such a way that chains differing by only one nucleotide in length can be separated?

If two DNA samples are exactly the same,what would you expect to happen if you exposed both to the same mixture of primers and then carried out the polymerase chain reaction (PCR)?

Which of the following is most likely to elute first from a gel filtration column if each of them is essentially globular?

A(n)_________ is responsible for collecting light rays previously focused on the specimen.

In which technique is radioactivity detected by mixing the sample in a vial with a special fluid that contains compounds that emit light if struck by a beta particle? The amount of light emitted in the fluid is a measure of the amount of radioactivity in sample.

What determines the identity and chemical properties of an atom?

What salt is most often used in selective precipitation experiments?

Another name for short,single-stranded nucleic acid molecules is _________.

Why does ion exchange chromatography depend on the pH of the medium?

The fractured surface of the specimen is coated with a heavy metal layer with the metal being deposited at an angle.A uniform carbon layer is deposited on top of the metal layer from directly overhead to cement the patches of metal into a solid layer.What is the name for this process?

What is the name of the process by which recombinant DNA gets into bacteria after being picked up from the surrounding medium?

Non-denaturing gel electrophoresis (PAGE)has the disadvantage that it ____________.

What is defined as viral-mediated gene transfer,which is a very widely used strategy to get genes into eukaryotic cells?

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA.The ends of the altered DNA are then ligated back together.What is the effect on the gene sequence?

What typically happens if the cells of a cell line are injected into susceptible lab animals?

The 3-D computerized reconstruction of a cell generated by cryoelectron tomography is called a(n)________.

Nucleic acid hybridization techniques are based on the observation that ______________.

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes, and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Exam 18: Techniques in Cell and Molecular Biology

Antibodies can distinguish between two polypeptides that differ by as little as __________.

_________ are established with cells that have been frozen in liquid nitrogen,thawed and then placed in a waiting culture medium.They are usually previously cultured cells that were frozen for storage.

How big are the sites recognized by most restriction enzymes?

What is used to separate DNA fragments in the cycle sequencing method of DNA sequencing in such a way that chains differing by only one nucleotide in length can be separated?

If two DNA samples are exactly the same,what would you expect to happen if you exposed both to the same mixture of primers and then carried out the polymerase chain reaction (PCR)?

Which of the following is most likely to elute first from a gel filtration column if each of them is essentially globular?

A(n)_________ is responsible for collecting light rays previously focused on the specimen.

In which technique is radioactivity detected by mixing the sample in a vial with a special fluid that contains compounds that emit light if struck by a beta particle? The amount of light emitted in the fluid is a measure of the amount of radioactivity in sample.

What determines the identity and chemical properties of an atom?

What salt is most often used in selective precipitation experiments?

Another name for short,single-stranded nucleic acid molecules is _________.

Why does ion exchange chromatography depend on the pH of the medium?

The fractured surface of the specimen is coated with a heavy metal layer with the metal being deposited at an angle.A uniform carbon layer is deposited on top of the metal layer from directly overhead to cement the patches of metal into a solid layer.What is the name for this process?

What is the name of the process by which recombinant DNA gets into bacteria after being picked up from the surrounding medium?

Non-denaturing gel electrophoresis (PAGE)has the disadvantage that it ____________.

What is defined as viral-mediated gene transfer,which is a very widely used strategy to get genes into eukaryotic cells?

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA.The ends of the altered DNA are then ligated back together.What is the effect on the gene sequence?

What typically happens if the cells of a cell line are injected into susceptible lab animals?

The 3-D computerized reconstruction of a cell generated by cryoelectron tomography is called a(n)________.

Nucleic acid hybridization techniques are based on the observation that ______________.

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes, and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters