Exam 18: Techniques in Cell and Molecular Biology

________ are atoms with the same number of protons and varying number of neutrons.

Why does the fracture plane usually pass through the center of cell membranes?

After exposing a DNA extract to phenol or a mixture of phenol and chloroform and centrifuging the solution,where are the proteins located?

Through what process did the existence of genes first become apparent?

What is the process of transferring single-stranded DNA from a gel to a nitrocellulose membrane called?

Which form of radiation is emitted by the most commonly used isotopes and by most biologically important isotopes?

__________ are established with cells that have been obtained directly from the organism;most of these cultures produced from animal cells are obtained from ________.

Nowadays,how are longer synthetic molecules of DNA synthesized for use in experimental procedures?

What property of proteins is used to purify them by selective precipitation?

A collection of cloned DNA fragments is called _________.

How are fragments already in a mass spectrometer further fragmented if this becomes necessary?

As solvent passes through a column,it is collected as it drips out of the bottom of the column into a series of tubes;each of these tubes is called a(n)____________.

After plant cells have been separated and grown in culture,they can grow into an undifferentiated clump of cells called a(n)__________.

With what molecules in a cell does osmium tetroxide primarily react?

What word or phrase describes what you get if the magnification power of the microscope exceeds the ability of the scope to resolve?

PCR employs a heat-stable DNA polymerase called ________,originally isolated from a bacterium that lives in hot springs at temperatures higher than 90°C.

In the PCR quantification procedure involving molecular beacons,what causes the fluorochrome to fluoresce?

What happens if a photographic emulsion is brought into close contact with radioactive source in a specimen?

Good fixatives are chemicals that _______ and ________ cellular macromolecules without producing artifacts.

Microinjection is a technique in which ____________.