Exam 18: Techniques in Cell and Molecular Biology

Which electron microscope technique below is used to view the surfaces of objects ranging in size from a virus to an animal's head and generates a three-dimensional image?

What advantage do images produced by freeze-fracturing and freeze-etching have over images generated with freeze-fracturing alone?

Whole blood from an animal that has been exposed to foreign materials (antigens)and then had the cells and clotting factors removed from it is called _________.

Why must a specimen that is to be viewed in the SEM have no water in it?

Nowadays,how are double-stranded molecules of DNA synthesized for use in experimental procedures?

What is the term for a variety of techniques in which a mixture of dissolved components is fractionated as it moves through a porous matrix?

What is the name of the special device used to section a frozen block of tissue?

When DNAs from two different organisms are incubated together,some duplexes are formed by DNA strands from the two species.What indicates that such duplexes are less stable than those between strands from the same organism?

If the photographic emulsion used in the autoradiography procedure is left in contact with the isotope for a longer period of time,what happens?

A plant cell that lacks a cell wall is called a(n)_________.

__________ is the ratio of the amount of the protein of interest to the total amount of total protein present in the sample.

Protein solubility in a given solution is based on ____________.

The process of learning about genotypes by studying mutant phenotypes is called ________.

Why do phenol and buffered saline separate into two phases when one centrifuges the mixture during the DNA extraction procedure?

In which chromatographic method are the charges of the proteins in question the basis of their purification?

A permanent collection of all of the sequences in the genome of a species is called a(n)_____.

A small,circular,double-stranded DNA molecule that is separate from the main bacterial chromosome is called a(n)___________.

Why have most clinical trials in which retroviruses have been used to introduce genes for the purpose of gene therapy in patients lacking that gene been unsuccessful?

A ________ is a culture in which the cells have undergone genetic modifications that allow them to grow indefinitely.

With what is the solvent in an SEM specimen's cells usually replaced?