Exam 18: Techniques in Cell and Molecular Biology

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What is the name of the procedure in which proteins separated on a polyacrylamide gel are transferred with the application of a current to a nitrocellulose filter placed against the gel and subsequently identified by their interaction with specific antibodies?

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What is a chelator?

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What technique distributes the contents of a homogenate into various layers according to the components' densities?

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An antibody molecule preparation made by a single colony or clone of antibody-producing cells would consist of large quantities of antibodies that all had exactly the same antigen-combining site.Such antibodies would be called _________ antibodies.

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Which of the following words is a correct description of image formation in the scanning electron microscope?

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About how big are the DNA fragments that can be placed into phage λ\lambda and cloned?

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What step must first be carried out before one can amplify RNA templates?

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What is required in order to study a protein's fine structure or function?

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When making a measurement in a spectrophotometer if you are illuminating a specimen with ultraviolet light,into what kind of container should it be placed?

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Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _________;each different B cell has membrane-bound antibodies with an affinity for __________.

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The amount of light passing through a solution unabsorbed or _________ is measured by ________ on the other side of the cuvette.

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How sensitive is gel electrophoresis when it is used to separate DNA?

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Which of the following is a technique used to isolate a particular organelle in bulk so that its function can be studied or so that an enzyme can be isolated from it?

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If you do site-directed mutagenesis of the coding region of a gene,which of the following might happen?

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In phage display,the antibody molecule is displayed on the _______.

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Which form of radiation is composed of electromagnetic radiation or photons?

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Cells that are capable of unlimited division are said to be _________.

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The magnification of a microscope is equal to __________.

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What method is typically used to dry specimens for scanning electron microscopy?

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To what are the reflection positions and intensities on the photographic plate of an X-ray crystallography experiment related?

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