Exam 9: DNA Replication and Recombination

You are studying a new virus with a DNA genome of 12 kb.It can synthesize DNA at a rate of 400 nucleotides per second.If the virus uses theta replication, how long will it take to replicate its genome?

Which of the following BEST describes heteroduplex DNA?

The nuclear genome of a single human cell (i.e., the entire diploid complement)contains about 6.6 billion (6.6 × 10⁹)base pairs of DNA.If synthesis at each replication fork occurs at an average rate of 50 nucleotides per second, all the DNA is replicated in 5 minutes.Assume that replication is initiated simultaneously at all origins.How many origins of replication exist in a human diploid genome?

In the diagram below, which letter indicates the 5' end of the leading strand?

The Holliday model describes which process?

All DNA polymerases synthesize new DNA by adding nucleotides to the _____ of the growing DNA chain.

Replication in archaebacteria is similar to:

DNA primase requires a _____ template and _____ nucleotides to initiate primer synthesis.

Why do eukaryotic chromosomes have multiple origins of replication, whereas prokaryotic chromosomes typically have only one origin?

Which different DNA polymerases are found in eukaryotic cells? What are their functions?

The nuclear genome of a single human cell (i.e., the entire diploid complement)contains about 6.6 billion (6.6 × 10⁹)base pairs of DNA.If synthesis at each replication fork occurs at an average rate of 50 nucleotides per second, all the DNA is replicated in 5 minutes.Assume that replication is initiated simultaneously at all origins.Assuming that the origins are approximately equally distributed across the chromosomes, what is the average number of origins per human chromosome?

You have a bacterial cell in which a mutation occurred.You observe that Okazaki fragments are created but RNA primers are not removed.Which gene is mutated?

The diagram below represents a replication fork.The leading strand is the _____ strand.

If a deletion occurs in a gene that encodes DNA polymerase I and no functional DNA polymerase I is produced, what will be the MOST likely consequence of this mutation?

What does "proofreading" refer to with regard to DNA replication?

What is the function of DNA ligase?

Okazaki fragments are found in all of the following EXCEPT:

DNA polymerases require all of the following for DNA replication EXCEPT:

Topoisomerases help DNA replication by:

There is a misconception that telomerase prevents the shortening of telomeres by just filling in the piece of RNA primer lost.What does telomerase actually do?