Question 1

DNA polymerase I and DNA polymerase III both have _____ activity, but only DNA polymerase I has _____ activity.

Accepted Answer

A) 5'$\rightarrow$3' exonuclease; 3'$\rightarrow$5' exonuclease 
B) 5'$\rightarrow$ 3' polymerase; 3'$\rightarrow$5' polymerase 
C) 3'$\rightarrow$5' polymerase; 5'$\rightarrow$3' polymerase 
D) 3'$\rightarrow$5' exonuclease; 5'$\rightarrow$3' exonuclease 
E) 5'$\rightarrow$ 3'polymerase; 3'$\rightarrow$5' exonuclease 
A) 5'$\rightarrow$3' exonuclease; 3'$\rightarrow$5' exonuclease 
B) 5'$\rightarrow$ 3' polymerase; 3'$\rightarrow$5' polymerase 
C) 3'$\rightarrow$5' polymerase; 5'$\rightarrow$3' polymerase 
D) 3'$\rightarrow$5' exonuclease; 5'$\rightarrow$3' exonuclease 
E) 5'$\rightarrow$ 3'polymerase; 3'$\rightarrow$5' exonuclease

Question 2

Meselson and Stahl showed that DNA is replicated by a _____ system.

Accepted Answer

A) conservative 
B) semiconservative 
C) dispersive 
D) semidispersive 
E) conservative in prokaryotes and dispersive in eukaryotes 
A) conservative 
B) semiconservative 
C) dispersive 
D) semidispersive 
E) conservative in prokaryotes and dispersive in eukaryotes

Question 3

What is the function of DNA gyrase?

Accepted Answer

A) Connects Okazaki fragments by sealing nicks in the sugar-phosphate backbone 
B) Unwinds the double helix by breaking the hydrogen bonding between the two strands at the replication fork 
C) Reduces the torsional strain that builds up ahead of the replication fork as a result of unwinding 
D) Binds to oriC and causes a short 
E) Prevents the formation of secondary structures within single-stranded DNA 
A) Connects Okazaki fragments by sealing nicks in the sugar-phosphate backbone 
B) Unwinds the double helix by breaking the hydrogen bonding between the two strands at the replication fork 
C) Reduces the torsional strain that builds up ahead of the replication fork as a result of unwinding 
D) Binds to oriC and causes a short 
E) Prevents the formation of secondary structures within single-stranded DNA

Question 4

Are Okazaki fragments formed on the leading strand during DNA replication? Explain your answer.

Accepted Answer

No, they are not.Okazaki fragments form

Question 5

You discovered a special dye that reveals the position of recombination sites on meiotic chromosomes.You use this dye to count the number of recombination sites and then compare this to the number of genetic exchanges that you can detect by looking at the segregation of markers across the genome.You find many more recombination sites as compared to genetic exchanges.Explain this result.

Accepted Answer

Recombination, whether by the Holliday m

Question 6

Which of the following is TRUE of DNA polymerases of eukaryotic cells?

Accepted Answer

A) The same DNA polymerase replicates mitochondrial, chloroplast, and nuclear DNA. 
B) There are only two different DNA polymerases that function in the process of replication. 
C) Some DNA polymerases have the ability to function in DNA repair mechanisms. 
D) All eukaryotic DNA polymerases have 3'$\rightarrow$5' exonuclease activity. 
E) Leading and lagging strand synthesis are performed by the same type of DNA polymerase. 
A) The same DNA polymerase replicates mitochondrial, chloroplast, and nuclear DNA. 
B) There are only two different DNA polymerases that function in the process of replication. 
C) Some DNA polymerases have the ability to function in DNA repair mechanisms. 
D) All eukaryotic DNA polymerases have 3'$\rightarrow$5' exonuclease activity. 
E) Leading and lagging strand synthesis are performed by the same type of DNA polymerase.

Question 7

DNA synthesis during replication is initiated from:

Accepted Answer

A) a free 5' OH. 
B) DNA primers. 
C) RNA primers. 
D) telomerase. 
E) DNA polymerase I. 
A) a free 5' OH. 
B) DNA primers. 
C) RNA primers. 
D) telomerase. 
E) DNA polymerase I.

Question 8

What type of synthesis occurs on the leading strand?

Accepted Answer

A) Conservative 
B) Dispersive 
C) Continuous 
D) Discontinuous 
E) Recombination 
A) Conservative 
B) Dispersive 
C) Continuous 
D) Discontinuous 
E) Recombination

Question 9

You have a bacterial cell in which a mutation occurred.You observe that Okazaki fragments are created but not linked as one continuous strand.Which gene is mutated?

Accepted Answer

A) DNS ligase 
B) DNA polymerase I 
C) DNA polymerase III 
D) DNA helicase 
E) SS binding proteins 
A) DNS ligase 
B) DNA polymerase I 
C) DNA polymerase III 
D) DNA helicase 
E) SS binding proteins

Question 10

_____ are tandemly repeated DNA sequences located at the ends of eukaryotic chromosomes.

Accepted Answer

A) Replication bubbles 
B) Telomeres 
C) Nucleosomes 
D) Licensing factors 
E) Holliday junctions 
A) Replication bubbles 
B) Telomeres 
C) Nucleosomes 
D) Licensing factors 
E) Holliday junctions

Question 11

Which of the following does NOT utilize bidirectional replication?

Accepted Answer

A) Theta model 
B) Archaebacteria 
C) Linear model 
D) Eukaryotes 
E) Bacteria 
A) Theta model 
B) Archaebacteria 
C) Linear model 
D) Eukaryotes 
E) Bacteria

Question 12

Suppose Meselson and Stahl had obtained the following results in their experiment.Which model of replication are these results consistent with?

Accepted Answer

A) Conservative replication only 
B) Semiconservative and conservative replication 
C) Semiconservative replication only 
D) Dispersive replication only 
E) Semiconservative and dispersive replication 
A) Conservative replication only 
B) Semiconservative and conservative replication 
C) Semiconservative replication only 
D) Dispersive replication only 
E) Semiconservative and dispersive replication

Question 13

In the diagram below, the arrow indicates the direction of movement of a replication fork.   a.Which strand, top or bottom, is the leading strand? Explain.
b.On which strand, top or bottom, would you expect to find Okazaki fragments? Explain.

Accepted Answer

a.The bottom strand, which has 5' to 3's

Question 14

Telomerase uses _____ to synthesize new DNA.

Accepted Answer

A) exonuclease activity 
B) a licensing factor 
C) strand invasion 
D) a DNA template 
E) an RNA template 
A) exonuclease activity 
B) a licensing factor 
C) strand invasion 
D) a DNA template 
E) an RNA template

Question 15

Explain the effect on DNA replication of mutations that destroy each of the following activities in DNA polymerase.Also, for each kind of mutation, how might you detect the effect in an in vitro replication reaction?
a.5' to 3' polymerase
b.5'to 3'exonuclease
c.3' to 5'exonuclease

Accepted Answer

a.If the 5' to 3'polymerase activity is

Question 16

List and describe the key events of DNA replication in their relative order for E.coli.

Accepted Answer

(1)Initiator proteins recognize and bind

Question 17

The leading and the lagging strands differ in that the:

Accepted Answer

A) leading strand is synthesized toward the replication fork and the lagging strand is synthesized in the opposite direction. 
B) leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5' end. 
C) lagging strand is synthesized continuously, whereas the leading strand is synthesized in short fragments that are ultimately attached to each other. 
D) leading strand is synthesized at twice the rate of the lagging strand. 
A) leading strand is synthesized toward the replication fork and the lagging strand is synthesized in the opposite direction. 
B) leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5' end. 
C) lagging strand is synthesized continuously, whereas the leading strand is synthesized in short fragments that are ultimately attached to each other. 
D) leading strand is synthesized at twice the rate of the lagging strand.

Question 18

The following is a depiction of a Holliday junction with the option of cutting on the horizontal plane or the vertical plane.

Accepted Answer

A) The horizontal plane will result in no recombination, and the vertical plane will result in crossover recombination. 
B) The horizontal plane will result in crossover recombination, and the vertical plane will result in no recombination. 
C) Both will result in no recombination. 
D) Both will result in crossover with crossover recombination. 
E) Which plane is cut is not important. 
A) The horizontal plane will result in no recombination, and the vertical plane will result in crossover recombination. 
B) The horizontal plane will result in crossover recombination, and the vertical plane will result in no recombination. 
C) Both will result in no recombination. 
D) Both will result in crossover with crossover recombination. 
E) Which plane is cut is not important.

Question 19

(a)Explain the significance of telomerase in cells of the germ line.(b)Explain the significance of telomerase in cancer cells.

Accepted Answer

(a)Telomerase allows for the ends of chr

Question 20

What is a heteroduplex region, and how is it created?

Accepted Answer

A heteroduplex DNA region is c

DNA polymerase I and DNA polymerase III both have _ activity, but only DNA polymerase I has _ activity.

Meselson and Stahl showed that DNA is replicated by a _____ system.

What is the function of DNA gyrase?

Are Okazaki fragments formed on the leading strand during DNA replication? Explain your answer.

Which of the following is TRUE of DNA polymerases of eukaryotic cells?

DNA synthesis during replication is initiated from:

What type of synthesis occurs on the leading strand?

You have a bacterial cell in which a mutation occurred.You observe that Okazaki fragments are created but not linked as one continuous strand.Which gene is mutated?

_____ are tandemly repeated DNA sequences located at the ends of eukaryotic chromosomes.

Which of the following does NOT utilize bidirectional replication?

Suppose Meselson and Stahl had obtained the following results in their experiment.Which model of replication are these results consistent with?

In the diagram below, the arrow indicates the direction of movement of a replication fork. a.Which strand, top or bottom, is the leading strand? Explain. b.On which strand, top or bottom, would you expect to find Okazaki fragments? Explain.

Telomerase uses _____ to synthesize new DNA.

Explain the effect on DNA replication of mutations that destroy each of the following activities in DNA polymerase.Also, for each kind of mutation, how might you detect the effect in an in vitro replication reaction? a.5' to 3' polymerase b.5'to 3'exonuclease c.3' to 5'exonuclease

List and describe the key events of DNA replication in their relative order for E.coli.

The leading and the lagging strands differ in that the:

The following is a depiction of a Holliday junction with the option of cutting on the horizontal plane or the vertical plane.

(a)Explain the significance of telomerase in cells of the germ line.(b)Explain the significance of telomerase in cancer cells.

What is a heteroduplex region, and how is it created?

Introduction to Genetics

Chromosomes and Cellular Reproduction

Basic Principles of Heredity

Sex Determination and Sex-Linked Characteristics

Linkage, Recombination, and Eukaryotic Gene Mapping

Chromosome Variation

Bacterial and Viral Genetic Systems

DNA- the Chemical Nature of the Gene

From DNA to Proteins- Transcription and Rna Processing

From DNA to Protein- Translation

Control of Gene Expression

Gene Mutations, Transposable Elements, and Dna Repair

Molecular Genetic Analysis and Biotechnology

Genomics and Proteomics

Cancer Genetics

Quantitative Genetics

Population and Evolutionary Genetics

Filters

Exam 9: DNA Replication and Recombination

DNA polymerase I and DNA polymerase III both have _____ activity, but only DNA polymerase I has _____ activity.

Meselson and Stahl showed that DNA is replicated by a _____ system.

What is the function of DNA gyrase?

Are Okazaki fragments formed on the leading strand during DNA replication? Explain your answer.

Which of the following is TRUE of DNA polymerases of eukaryotic cells?

DNA synthesis during replication is initiated from:

What type of synthesis occurs on the leading strand?

You have a bacterial cell in which a mutation occurred.You observe that Okazaki fragments are created but not linked as one continuous strand.Which gene is mutated?

_____ are tandemly repeated DNA sequences located at the ends of eukaryotic chromosomes.

Which of the following does NOT utilize bidirectional replication?

Suppose Meselson and Stahl had obtained the following results in their experiment.Which model of replication are these results consistent with?

In the diagram below, the arrow indicates the direction of movement of a replication fork. a.Which strand, top or bottom, is the leading strand? Explain. b.On which strand, top or bottom, would you expect to find Okazaki fragments? Explain.

Telomerase uses _____ to synthesize new DNA.

Explain the effect on DNA replication of mutations that destroy each of the following activities in DNA polymerase.Also, for each kind of mutation, how might you detect the effect in an in vitro replication reaction? a.5' to 3' polymerase b.5'to 3'exonuclease c.3' to 5'exonuclease

List and describe the key events of DNA replication in their relative order for E.coli.

The leading and the lagging strands differ in that the:

The following is a depiction of a Holliday junction with the option of cutting on the horizontal plane or the vertical plane.

(a)Explain the significance of telomerase in cells of the germ line.(b)Explain the significance of telomerase in cancer cells.

What is a heteroduplex region, and how is it created?

Introduction to Genetics

Chromosomes and Cellular Reproduction

Basic Principles of Heredity

Sex Determination and Sex-Linked Characteristics

Linkage, Recombination, and Eukaryotic Gene Mapping

Chromosome Variation

Bacterial and Viral Genetic Systems

DNA- the Chemical Nature of the Gene

From DNA to Proteins- Transcription and Rna Processing

From DNA to Protein- Translation

Control of Gene Expression

Gene Mutations, Transposable Elements, and Dna Repair

Molecular Genetic Analysis and Biotechnology

Genomics and Proteomics

Cancer Genetics

Quantitative Genetics

Population and Evolutionary Genetics

Filters

DNA polymerase I and DNA polymerase III both have _ activity, but only DNA polymerase I has _ activity.