Question 1

Which one of the following statements is NOT true for all E.coli DNA polymerases?

Accepted Answer

A) They require a primer to initiate synthesis. 
B) They use dNTPs to synthesize new DNA. 
C) They produce newly synthesized strands that are complementary and antiparallel to the template strands. 
D) They possess 5' $\rightarrow$3'exonuclease activity. 
E) They synthesize in the 5'$\rightarrow$3'direction by adding nucleotides to a 3' -OH group. 
A) They require a primer to initiate synthesis. 
B) They use dNTPs to synthesize new DNA. 
C) They produce newly synthesized strands that are complementary and antiparallel to the template strands. 
D) They possess 5' $\rightarrow$3'exonuclease activity. 
E) They synthesize in the 5'$\rightarrow$3'direction by adding nucleotides to a 3' -OH group.

Question 2

Which of the following typically only has one origin of replication?

Accepted Answer

A) Prokaryotes 
B) Eukaryotes 
C) Linear model of replication 
D) Humans 
E) Plants 
A) Prokaryotes 
B) Eukaryotes 
C) Linear model of replication 
D) Humans 
E) Plants

Question 3

The proofreading function of DNA polymerases involves _____ activity.

Accepted Answer

A) 5'$\rightarrow$ 3'exonuclease 
B) 3' $\rightarrow$ 5' exonuclease 
C) 5' $\rightarrow$ 3' telomerase 
D) 3' $\rightarrow$ 5'telomerase 
E) 5' $\rightarrow$ 3'gyrase 
A) 5'$\rightarrow$ 3'exonuclease 
B) 3' $\rightarrow$ 5' exonuclease 
C) 5' $\rightarrow$ 3' telomerase 
D) 3' $\rightarrow$ 5'telomerase 
E) 5' $\rightarrow$ 3'gyrase

Question 4

You learn that a Mars lander has retrieved a bacterial sample from the polar ice caps.To determine how the Mars bacteria replicate their DNA, you obtain a sample of these bacteria and perform the same kind of experiment that Meselson and Stahl did.Based on the following equilibrium density gradient centrifugation results, what type of replication would you propose for these new bacteria?

Accepted Answer

A) Conservative 
B) Semiconservative 
C) Dispersive 
D) Semiconservative or dispersive 
E) Conservative or dispersive 
A) Conservative 
B) Semiconservative 
C) Dispersive 
D) Semiconservative or dispersive 
E) Conservative or dispersive

Question 5

Which of the following is a protein that facilitates the termination of replication in E.coli?

Accepted Answer

A) Telomerase 
B) DNA gyrase 
C) Tus 
D) Primase 
E) Topoisomerase 
A) Telomerase 
B) DNA gyrase 
C) Tus 
D) Primase 
E) Topoisomerase

Question 6

What would be a likely result of expressing telomerase in somatic cells?

Accepted Answer

A) Premature aging 
B) Cancer 
C) Lower rates of replication 
D) Immortality of gametes 
E) Early termination of replication 
A) Premature aging 
B) Cancer 
C) Lower rates of replication 
D) Immortality of gametes 
E) Early termination of replication

Question 7

Why did the Meselson and Stahl experiment require two rounds of replication?

Accepted Answer

The first round of replication, which pr

Question 8

What is the function of single-strand-binding proteins?

Accepted Answer

A) Connects Okazaki fragments by sealing nicks in the sugar-phosphate backbone 
B) Unwinds the double helix by breaking the hydrogen bonding between the two strands at the replication fork 
C) Reduces the torsional strain that builds up ahead of the replication fork as a result of unwinding 
D) Binds to oriC and causes a short 
E) Prevents the formation of secondary structures within single-stranded DNA 
A) Connects Okazaki fragments by sealing nicks in the sugar-phosphate backbone 
B) Unwinds the double helix by breaking the hydrogen bonding between the two strands at the replication fork 
C) Reduces the torsional strain that builds up ahead of the replication fork as a result of unwinding 
D) Binds to oriC and causes a short 
E) Prevents the formation of secondary structures within single-stranded DNA

Question 9

What does the term "noncrossover recombinant" mean with respect to homologous recombination?

Accepted Answer

Such a recombinant is therefore said to

Question 10

Which of the following is a necessary step in the Holliday model of recombination?

Accepted Answer

A) DNA primase generates an RNA primer. 
B) The RNA template of telomerase binds to the telomere. 
C) Topoisomerases aid in supercoiling. 
D) DNA polymerase $\alpha$ initiates DNA synthesis. 
E) A single-strand break occurs in the DNA molecule. 
A) DNA primase generates an RNA primer. 
B) The RNA template of telomerase binds to the telomere. 
C) Topoisomerases aid in supercoiling. 
D) DNA polymerase $\alpha$ initiates DNA synthesis. 
E) A single-strand break occurs in the DNA molecule.

Question 11

Which of the following enzymes do NOT aid in unwinding of DNA for replication?

Accepted Answer

A) Helicase 
B) Single-stranded binding proteins 
C) Primase 
D) Gyrase 
E) Topoisomerase 
A) Helicase 
B) Single-stranded binding proteins 
C) Primase 
D) Gyrase 
E) Topoisomerase

Question 12

For which of the following is the "end-replication problem" relevant?

Accepted Answer

A) Circular DNA 
B) Linear chromosomes 
C) The centromere region of a chromosome 
D) Bacterial chromosome 
E) Theta model of replication 
A) Circular DNA 
B) Linear chromosomes 
C) The centromere region of a chromosome 
D) Bacterial chromosome 
E) Theta model of replication

Question 13

What would be the phenotype of a differentiated cell that is a telomerase mutant? Would it be viable?

Accepted Answer

Since telomerase is not requir

Question 14

Which activity is NOT associated with DNA polymerases?

Accepted Answer

A) Ability to attach a DNA nucleotide to the 3'end of previously incorporated DNA nucleotide 
B) Ability to excise a newly incorporated nucleotide that does not match the template strand 
C) Ability to "read" a template strand 3' to 5'and synthesize a complementary strand 
D) Ability to synthesize a DNA from scratch without a primer 
E) Ability to synthesize new DNA in a 5'to 3'direction 
A) Ability to attach a DNA nucleotide to the 3'end of previously incorporated DNA nucleotide 
B) Ability to excise a newly incorporated nucleotide that does not match the template strand 
C) Ability to "read" a template strand 3' to 5'and synthesize a complementary strand 
D) Ability to synthesize a DNA from scratch without a primer 
E) Ability to synthesize new DNA in a 5'to 3'direction

Question 15

Suppose that some cells are grown in culture in the presence of radioactive nucleotides for many generations so that both strands of every DNA molecule include radioactive nucleotides.The cells are then harvested and placed in new medium with nucleotides that are not radioactive so that newly synthesized DNA will not be radioactive.What proportion of DNA molecules will contain radioactivity after two rounds of replication?

Accepted Answer

A) 0 
B) 1/8 
C) 1/4 
D) 1/3 
E) 1/2 
A) 0 
B) 1/8 
C) 1/4 
D) 1/3 
E) 1/2

Question 16

Explain the need for and mechanism of licensing of DNA replication.

Accepted Answer

In order for the large genomes of eukary

Question 17

Telomerase activity is MOST likely to be found in which cells in humans?

Accepted Answer

A) Red blood cells 
B) Muscle cells 
C) Neurons 
D) Germ line 
E) Any type of somatic cell 
A) Red blood cells 
B) Muscle cells 
C) Neurons 
D) Germ line 
E) Any type of somatic cell

Question 18

Both gyrase and helicase help to open up the DNA for replication.How do they differ?

Accepted Answer

Gyrase unwinds the two DNA str

Question 19

What type of bonds does DNA ligase create between adjacent nucleotides?

Accepted Answer

A) Hydrogen 
B) Phosphodiester 
C) Ionic 
D) Metallic 
E) Ribonucleotide 
A) Hydrogen 
B) Phosphodiester 
C) Ionic 
D) Metallic 
E) Ribonucleotide

Which one of the following statements is NOT true for all E.coli DNA polymerases?

Which of the following typically only has one origin of replication?

The proofreading function of DNA polymerases involves _____ activity.

Which of the following is a protein that facilitates the termination of replication in E.coli?

What would be a likely result of expressing telomerase in somatic cells?

Why did the Meselson and Stahl experiment require two rounds of replication?

What is the function of single-strand-binding proteins?

What does the term "noncrossover recombinant" mean with respect to homologous recombination?

Which of the following is a necessary step in the Holliday model of recombination?

Which of the following enzymes do NOT aid in unwinding of DNA for replication?

For which of the following is the "end-replication problem" relevant?

What would be the phenotype of a differentiated cell that is a telomerase mutant? Would it be viable?

Which activity is NOT associated with DNA polymerases?

Explain the need for and mechanism of licensing of DNA replication.

Telomerase activity is MOST likely to be found in which cells in humans?

Both gyrase and helicase help to open up the DNA for replication.How do they differ?

What type of bonds does DNA ligase create between adjacent nucleotides?

Introduction to Genetics

Chromosomes and Cellular Reproduction

Basic Principles of Heredity

Sex Determination and Sex-Linked Characteristics

Linkage, Recombination, and Eukaryotic Gene Mapping

Chromosome Variation

Bacterial and Viral Genetic Systems

DNA- the Chemical Nature of the Gene

From DNA to Proteins- Transcription and Rna Processing

From DNA to Protein- Translation

Control of Gene Expression

Gene Mutations, Transposable Elements, and Dna Repair

Molecular Genetic Analysis and Biotechnology

Genomics and Proteomics

Cancer Genetics

Quantitative Genetics

Population and Evolutionary Genetics

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Exam 9: DNA Replication and Recombination

Which one of the following statements is NOT true for all E.coli DNA polymerases?

Which of the following typically only has one origin of replication?

The proofreading function of DNA polymerases involves _____ activity.

Which of the following is a protein that facilitates the termination of replication in E.coli?

What would be a likely result of expressing telomerase in somatic cells?

Why did the Meselson and Stahl experiment require two rounds of replication?

What is the function of single-strand-binding proteins?

What does the term "noncrossover recombinant" mean with respect to homologous recombination?

Which of the following is a necessary step in the Holliday model of recombination?

Which of the following enzymes do NOT aid in unwinding of DNA for replication?

For which of the following is the "end-replication problem" relevant?

What would be the phenotype of a differentiated cell that is a telomerase mutant? Would it be viable?

Which activity is NOT associated with DNA polymerases?

Explain the need for and mechanism of licensing of DNA replication.

Telomerase activity is MOST likely to be found in which cells in humans?

Both gyrase and helicase help to open up the DNA for replication.How do they differ?

What type of bonds does DNA ligase create between adjacent nucleotides?

Introduction to Genetics

Chromosomes and Cellular Reproduction

Basic Principles of Heredity

Sex Determination and Sex-Linked Characteristics

Linkage, Recombination, and Eukaryotic Gene Mapping

Chromosome Variation

Bacterial and Viral Genetic Systems

DNA- the Chemical Nature of the Gene

From DNA to Proteins- Transcription and Rna Processing

From DNA to Protein- Translation

Control of Gene Expression

Gene Mutations, Transposable Elements, and Dna Repair

Molecular Genetic Analysis and Biotechnology

Genomics and Proteomics

Cancer Genetics

Quantitative Genetics

Population and Evolutionary Genetics

Filters