Exam 15: Recombinant Dna Technology

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore, they can be transmitted like phages, but they can exist and replicate in a cell like plasmids.

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for E. M. Southern, the person who developed the procedure.

The three steps that take place in each cycle during PCR occur in which order?

A __________ is a DNA molecule used in hybridization reactions to detect the presence of a particular gene in separated DNA fragments.

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

Which of the following is not part of a yeast artificial chromosome (YAC)?

___________ is a bacterial plasmid vector.

A genomic __________ is a sufficiently large collection of recombinant DNA molecules in which the inserted sequences together represent the entire genome of an organism.

The most frequently chosen prokaryotic host in cloning techniques is

Which of the following can be used as vectors for cloning DNA fragments?

A PCR procedure that allows a determination of the amount of a particular DNA fragment that is present in a sample is called

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

Which of the following best describes the basis for separation of DNA fragments during agarose gel electrophoresis?

Promoters for genes that code for proteins can be isolated from a cDNA library.

A (n) __________ vector is a plasmid that can be replicated in several different organisms because it has at least one origin of replication that will function in each host.

Cloning a gene involves all of the following except

Some plasmid vectors have incorporated the regulatory sequences of the lactose operon so that the expression of the recombinant gene can be induced at the appropriate time.

A DNA molecule used to carry a foreign gene into a host organism is called a

Genetic engineering methods have been used to produce vaccines.

Plasmid cloning vector DNA is usually introduced into bacterial hosts by