Question 1

Genetically engineered mice can now produce fully human monoclonal antibodies.

Accepted Answer

A) True 
 B)False

Question 2

Antibiotics incorporated into the culture medium can

Accepted Answer

A)  select against organisms that have not incorporated the plasmid. 
B)  select against organisms that have incorporated a plasmid not containing the desired gene. 
C)  enhance production of recombinant proteins. 
D)  select against organisms that have not incorporated the plasmid and select against organisms that have incorporated a plasmid not containing the desired gene 
A)  select against organisms that have not incorporated the plasmid. 
B)  select against organisms that have incorporated a plasmid not containing the desired gene. 
C)  enhance production of recombinant proteins. 
D)  select against organisms that have not incorporated the plasmid and select against organisms that have incorporated a plasmid not containing the desired gene

Question 3

A(n)__________ __________ is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb) pieces of foreign DNA into a host organism.

Accepted Answer

artificial

Question 4

Restriction endonucleases in bacteria may have evolved in order to

Accepted Answer

A)  carry out natural genetic engineering. 
B)  protect the bacteria from infection by viruses. 
C)  use nucleic acids as a food (energy) source. 
D)  all of the choices 
A)  carry out natural genetic engineering. 
B)  protect the bacteria from infection by viruses. 
C)  use nucleic acids as a food (energy) source. 
D)  all of the choices

Question 5

In _____________, cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Accepted Answer

The answer of In _____________, cells are mixed with recombinant...

Question 6

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

Accepted Answer

A) True 
 B)False

Question 7

An enzyme that cleaves internal phosphodiester bonds of a DNA molecule is a (n)

Accepted Answer

A)  exonuclease. 
B)  endonuclease. 
C)  ligase. 
D)  methylase. 
A)  exonuclease. 
B)  endonuclease. 
C)  ligase. 
D)  methylase.

Question 8

Movement of charged molecules in an electrical field, which is used to separate nucleic acid fragments for recombinant DNA work, is called

Accepted Answer

A)  iontophoresis. 
B)  nucleophoresis. 
C)  electrophoresis. 
D)  plasmaphoresis. 
A)  iontophoresis. 
B)  nucleophoresis. 
C)  electrophoresis. 
D)  plasmaphoresis.

Question 9

The production of large quantities of a particular DNA sequence is known as gene __________________.

Accepted Answer

The answer of The production of large quantities of a...

Question 10

Derivatives of the bacterial Ti plasmid have been used to deliver genes to the chromosomes of plants.

Accepted Answer

A) True 
 B)False

Exam 15: Recombinant Dna Technology

Genetically engineered mice can now produce fully human monoclonal antibodies.

Antibiotics incorporated into the culture medium can

A(n)__________ __________ is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb) pieces of foreign DNA into a host organism.

Restriction endonucleases in bacteria may have evolved in order to

In _____________, cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

An enzyme that cleaves internal phosphodiester bonds of a DNA molecule is a (n)

Movement of charged molecules in an electrical field, which is used to separate nucleic acid fragments for recombinant DNA work, is called

The production of large quantities of a particular DNA sequence is known as gene __________________.

Derivatives of the bacterial Ti plasmid have been used to deliver genes to the chromosomes of plants.

The Evolution of Microorganisms and Microbiology

The Study of Microbial Structure: Microscopy and Specimen Preparation

Bacteria and Archaea

Eukaryotic Cell Structure and Function

Viruses and Other Acellular Infectious Agents

Microbial Nutrition

Microbial Growth

Control of Microorganisms in the Environment

Introduction to Metabolism

Catabolism Energy Release and Conservation

Anabolism the Use of Energy in Biosynthesis

Genes Structure, Replication, and Expression

Microbial Genetics Regulation of Gene Expression

Microbial Genetics Mechanisms of Genetic Variation

Microbial Genomics

Microbial Taxonomy and the Evolution of Diversity

The Archaea

Bacteria the Deinococci and Nonproteobacteria Gram Negatives

Bacteria the Proteobacteria

Bacteria the Low G+C Gram Positives

Bacteria the High G+C Gram Positives

The Protists

The Fungi Eumycota

The Viruses

Biogeochemical Cycling

Methods in Microbial Ecology

Microorganisms in Marine and Freshwater Ecosystems

Microorganisms in Terrestrial Ecosystems

Microbial Interactions

Infection and Pathogenicity

Nonspecific Innate Host Resistance

Specific Adaptive Immunity

Antimicrobial Chemotherapy

Clinical Microbiology and Immunology

Epidemiology and Public Health Microbiology

Human Diseases Caused by Viruses and Prions

Human Diseases Caused by Bacteria

Human Diseases Caused by Fungi and Protists

Microbiology of Food

Industrial Microbiology

Applied Environmental Microbiology

Filters

A(n) is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb) pieces of foreign DNA into a host organism.