Exam 7: Dna Structure and Replication

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Briefly describe the Meselson and Stahl experiment that indicated that DNA replication is semiconservative. How would their results have differed if DNA replication were actually conservative in nature?

(Essay)

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Question 41

What are the three steps of each PCR cycle?

(Short Answer)

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Question 42

What characteristic of the polymerase isolated from Thermus aquaticus makes it unique and highly useful for maintaining the enzyme efficiency during PCR?

(Short Answer)

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Question 43

What is the term for the daughter strand synthesized continuously during DNA replication?

(Short Answer)

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Question 44

Why would DNA synthesis occur in both directions from an origin of replication?

(Multiple Choice)

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Question 45

________is the term for a multiprotein complex in which a core enzyme is associated with additional protein components leading to its function.

(Short Answer)

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Question 46

Which of the following temperature cycles would you expect to see in a standard polymerase chain reaction (from denaturation to annealing to extension)?

(Multiple Choice)

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Question 47

Describe the function of the oriC. What do you predict will happen to the cell if 100 bp between the 13- mer and 9- mer sequence is deleted as indicated in the figure?

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Question 48

The process by which Pneumococcus transfers DNA between living type RII and heat- killed type SIII cells is known as ________.

(Multiple Choice)

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Question 49

Where would you expect to find telomerase activity?

(Multiple Choice)

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Question 50

You identify a cell in which DNA polymerase III is functional, but it seems to exhibit extremely low processivity. This is likely a defect in what structure?

(Multiple Choice)

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Question 51

If there is 24% guanine in a DNA molecule, then there is________ cytosine.

(Multiple Choice)

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Question 52

Why are next generation DNA sequencing technologies known as sequencing- by- synthesis?

(Multiple Choice)

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Question 53

Which of the following are classified as pyrimidines?

(Multiple Choice)

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Question 54

Which arrow(s) point(s) to the location of helicase in the diagram shown?

(Multiple Choice)

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Question 55

A portion of one strand of DNA has the sequence 5' AATGGCTTA 3'. If this strand is used as a template for DNA replication, which of the following correctly depicts the sequence of the newly synthesized strand in the direction in which it will be synthesized?

(Multiple Choice)

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Question 56

Which functional groups have been altered in a ddNTP compared to a dNTP?

(Multiple Choice)

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Question 57

Showing 41 - 57 of 57

Briefly describe the Meselson and Stahl experiment that indicated that DNA replication is semiconservative. How would their results have differed if DNA replication were actually conservative in nature?

What are the three steps of each PCR cycle?

What characteristic of the polymerase isolated from Thermus aquaticus makes it unique and highly useful for maintaining the enzyme efficiency during PCR?

What is the term for the daughter strand synthesized continuously during DNA replication?

Why would DNA synthesis occur in both directions from an origin of replication?

________is the term for a multiprotein complex in which a core enzyme is associated with additional protein components leading to its function.

Which of the following temperature cycles would you expect to see in a standard polymerase chain reaction (from denaturation to annealing to extension)?

Describe the function of the oriC. What do you predict will happen to the cell if 100 bp between the 13- mer and 9- mer sequence is deleted as indicated in the figure?

The process by which Pneumococcus transfers DNA between living type RII and heat- killed type SIII cells is known as ________.

Where would you expect to find telomerase activity?

You identify a cell in which DNA polymerase III is functional, but it seems to exhibit extremely low processivity. This is likely a defect in what structure?

If there is 24% guanine in a DNA molecule, then there is________ cytosine.

Why are next generation DNA sequencing technologies known as sequencing- by- synthesis?

Which of the following are classified as pyrimidines?

Which arrow(s) point(s) to the location of helicase in the diagram shown?

A portion of one strand of DNA has the sequence 5' AATGGCTTA 3'. If this strand is used as a template for DNA replication, which of the following correctly depicts the sequence of the newly synthesized strand in the direction in which it will be synthesized?

Which functional groups have been altered in a ddNTP compared to a dNTP?

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Inheritance Patterns of Single Genes and Gene Interaction

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Analysis of Gene Function by Forward Genetics and Reverse Genetics

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