Question 1

Intestinal parasites can be identified by observing stool samples for the presence of

Accepted Answer

A)  cysts. 
B)  antibodies. 
C)  LPS. 
D)  blood. 
A)  cysts. 
B)  antibodies. 
C)  LPS. 
D)  blood.

Question 2

ALL positive HIV-EIA tests must be confirmed by another independent test,usually the HIV Western blot.

Accepted Answer

A) True 
 B)False

Question 3

The disk-diffusion test is used for

Accepted Answer

A)  assessing antimicrobial activity. 
B)  the identification of Escherichia coli. 
C)  the identification of viral plaques. 
D)  the detection of antigen. 
A)  assessing antimicrobial activity. 
B)  the identification of Escherichia coli. 
C)  the identification of viral plaques. 
D)  the detection of antigen.

Question 4

When using the EIA test for HIV screening,BOTH false negative and false positive results are possible.

Accepted Answer

A) True 
 B)False

Question 5

SYBR Green,commonly used in qPCR,binds nonspecifically to

Accepted Answer

A)  ssDNA 
B)  dsDNA 
C)  ssRNA 
D)  dsRNA 
A)  ssDNA 
B)  dsDNA 
C)  ssRNA 
D)  dsRNA

Question 6

Antibody titer can be measured by

Accepted Answer

A)  negative microscopy. 
B)  agglutination. 
C)  plate counts done after isolating the pathogen. 
D)  polymerase chain reaction. 
A)  negative microscopy. 
B)  agglutination. 
C)  plate counts done after isolating the pathogen. 
D)  polymerase chain reaction.

Question 7

MOST microbiology research laboratories maintain BSL-2 standards.

Accepted Answer

A) True 
 B)False

Question 8

Explain the principle difference between direct agglutination and passive agglutination.

Accepted Answer

Direct agglutination is clumping formed

Question 9

To detect antibodies in human serum,the direct EIA is most commonly employed.

Accepted Answer

A) True 
 B)False

Question 10

Bacteremia is a term used

Accepted Answer

A)  to indicate the presence of bacteria in the blood. 
B)  synonymously with septicemia. 
C)  when the same organism is isolated from more than one site. 
D)  to indicate the presence of bacteria in the lungs. 
A)  to indicate the presence of bacteria in the blood. 
B)  synonymously with septicemia. 
C)  when the same organism is isolated from more than one site. 
D)  to indicate the presence of bacteria in the lungs.

Question 11

Nucleic acid probes are NOT suitable for the detection of viruses.

Accepted Answer

A) True 
 B)False

Question 12

An Etest® is a clinical tool used for the determination of

Accepted Answer

A)  enteric presence. 
B)  Escherichia coli O157:H7 presence. 
C)  the minimum inhibitory concentration (MI
C)  value of an antimicrobial agent. 
D)  pathogenic enzyme activity. 
A)  enteric presence. 
B)  Escherichia coli O157:H7 presence. 
C)  the minimum inhibitory concentration (MI
C)  value of an antimicrobial agent. 
D)  pathogenic enzyme activity.

Question 13

Propose a series of procedures to determine whether a patient has a urinary tract infection.

Accepted Answer

Direct microscopic examination of urine

Question 14

EMB agar preferentially selects for the growth of ________ bacteria.

Accepted Answer

A)  gram-positive 
B)  gram-negative 
C)  gram-variable 
D)  both gram-positive and gram-negative 
A)  gram-positive 
B)  gram-negative 
C)  gram-variable 
D)  both gram-positive and gram-negative

Question 15

Some antibiotics inhibit protein synthesis by disruption of translation through interactions with the

Accepted Answer

A)  ribosome. 
B)  protein. 
C)  DNA. 
D)  chromosome. 
A)  ribosome. 
B)  protein. 
C)  DNA. 
D)  chromosome.

Question 16

Obligately anaerobic normal flora are found in ________,which is an/are anoxic habitat(s)of the body.

Accepted Answer

A)  the kidneys 
B)  the skin 
C)  portions of the oral cavity 
D)  the lungs 
A)  the kidneys 
B)  the skin 
C)  portions of the oral cavity 
D)  the lungs

Question 17

A positive antibody titer indicates

Accepted Answer

A)  previous exposure to an antigen. 
B)  active infection. 
C)  latent disease. 
D)  a healthy individual. 
A)  previous exposure to an antigen. 
B)  active infection. 
C)  latent disease. 
D)  a healthy individual.

Question 18

Some infectious agents,such as Neisseria gonorrhoeae,do NOT elicit a systemic immune response.

Accepted Answer

A) True 
 B)False

Question 19

Most drug-resistant bacteria isolated from patients contain a(n)

Accepted Answer

A)  R plasmid. 
B)  T1 phage. 
C)  reverse transcriptase. 
D)  β-lactam. 
A)  R plasmid. 
B)  T1 phage. 
C)  reverse transcriptase. 
D)  β-lactam.

Question 20

Discuss how recent advances in the molecular biology of nucleic acids have revolutionized methods used for the identification of infectious disease agents and how they are favored over traditional biochemical tests.

Accepted Answer

Molecular biology techniques that genera

Exam 27: Diagnostic Microbiology

Intestinal parasites can be identified by observing stool samples for the presence of

ALL positive HIV-EIA tests must be confirmed by another independent test,usually the HIV Western blot.

The disk-diffusion test is used for

When using the EIA test for HIV screening,BOTH false negative and false positive results are possible.

SYBR Green,commonly used in qPCR,binds nonspecifically to

Antibody titer can be measured by

MOST microbiology research laboratories maintain BSL-2 standards.

Explain the principle difference between direct agglutination and passive agglutination.

To detect antibodies in human serum,the direct EIA is most commonly employed.

Bacteremia is a term used

Nucleic acid probes are NOT suitable for the detection of viruses.

An Etest® is a clinical tool used for the determination of

Propose a series of procedures to determine whether a patient has a urinary tract infection.

EMB agar preferentially selects for the growth of ________ bacteria.

Some antibiotics inhibit protein synthesis by disruption of translation through interactions with the

Obligately anaerobic normal flora are found in ________,which is an/are anoxic habitat(s)of the body.

A positive antibody titer indicates

Some infectious agents,such as Neisseria gonorrhoeae,do NOT elicit a systemic immune response.

Most drug-resistant bacteria isolated from patients contain a(n)

Discuss how recent advances in the molecular biology of nucleic acids have revolutionized methods used for the identification of infectious disease agents and how they are favored over traditional biochemical tests.

Microorganisms and Microbiology

Microbial Cell Structure and Function

Microbial Metabolism

Molecular Biology

Microbial Growth and Control

Microbial Genomics

Metabolic Regulation

Viruses and Virology

Viral Genomes and Diversity

Genetics of Bacteria and Archaea

Genetic Engineering and Biotechnology

Microbial Evolution and Systematics

Metabolic Diversity of Microorganisms

Functional Diversity of Bacteria

Diversity of Bacteria

Diversity of Archaea

Diversity of Eukaryotic Microorganisms

Methods in Microbial Ecology

Microbial Ecosystems

Nutrient Cycles

Microbiology of the Built Environment

Microbial Symbioses

Microbial Interactions With Humans

Immunity and Host Defense

Immune Mechanisms

Molecular Immunology

Epidemiology

Person-To-Person Bacterial and Viral Diseases

Vectorborne and Soilborne Bacterial and Viral Diseases

Water and Food As Vehicles of Bacterial Diseases

Eukaryotic Pathogens: Fungal and Parasitic Diseases

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