Question 1

Standard Precautions include:

Accepted Answer

A) using barrier devices to prevent exposure to skin and mucous membranes. 
B) recapping needles by hand. 
C) storing drinks but not food in laboratory refrigerators. 
D) discarding sharps in plastic biohazard bags. 
A) using barrier devices to prevent exposure to skin and mucous membranes. 
B) recapping needles by hand. 
C) storing drinks but not food in laboratory refrigerators. 
D) discarding sharps in plastic biohazard bags.

Question 2

A cleaning solution used on the venipuncture site to avoid skin contamination of the blood sample is called a(n):

Accepted Answer

A) surfactant. 
B) disinfectant. 
C) biocide. 
D) antiseptic. 
A) surfactant. 
B) disinfectant. 
C) biocide. 
D) antiseptic.

Question 3

Applying a chemical to the surface of the laboratory bench to remove the contaminating microorganisms is considered:

Accepted Answer

A) sterilization. 
B) disinfection. 
C) decontamination. 
D) pasteurization. 
A) sterilization. 
B) disinfection. 
C) decontamination. 
D) pasteurization.

Question 4

The agency that requires health care facilities to provide employees with all devices and mechanisms necessary to protect them from the hazards encountered in the laboratory is:

Accepted Answer

A) Department of Health and Environmental Control (DHE
C) . 
B) Occupational Safety and Health Administration (OSH
A) . 
C) Centers for Disease Control and Prevention (CD
C) . 
D) Health Emergency Planning Advisor (HEP
A) . 
A) Department of Health and Environmental Control (DHE
C) . 
B) Occupational Safety and Health Administration (OSH
A) . 
C) Centers for Disease Control and Prevention (CD
C) . 
D) Health Emergency Planning Advisor (HEP
A) .

Question 5

Information concerning a chemical product's health hazards,toxicity,and disposal procedures is:

Accepted Answer

A) found in the manufacturer's material safety data sheets (MSDS). 
B) found on the product label. 
C) proprietary information and only released at the manufacturer's discretion. 
D) obtained through the manufacturer's technical support division;acquiring access to that information is the responsibility of the laboratory worker. 
A) found in the manufacturer's material safety data sheets (MSDS). 
B) found on the product label. 
C) proprietary information and only released at the manufacturer's discretion. 
D) obtained through the manufacturer's technical support division;acquiring access to that information is the responsibility of the laboratory worker.

Question 6

Chemicals used for disinfection that are capable of disrupting cellular membranes and reduce the surface tension through cationic disruption of water molecules are termed:

Accepted Answer

A) alcohols. 
B) bleach. 
C) iodine or heavy metal compounds. 
D) quaternary ammonium compounds. 
A) alcohols. 
B) bleach. 
C) iodine or heavy metal compounds. 
D) quaternary ammonium compounds.

Question 7

Minimal protection of specimen,personnel,and environment is accomplished with which class of biological safety cabinet?

Accepted Answer

A) Class I 
B) Class II 
C) Class III 
D) Class IV 
A) Class I 
B) Class II 
C) Class III 
D) Class IV

Question 8

Biosafety Level 1 agents:

Accepted Answer

A) include those that are the common agents of an infectious disease. 
B) require only standard good laboratory techniques. 
C) include Mycobacterium tuberculosis,M.gordonae,and Brucella. 
D) require the use of maximum containment facilities. 
A) include those that are the common agents of an infectious disease. 
B) require only standard good laboratory techniques. 
C) include Mycobacterium tuberculosis,M.gordonae,and Brucella. 
D) require the use of maximum containment facilities.

Question 9

The primary action in hand washing that prevents the distribution or transmission of microorganisms is:

Accepted Answer

A) the amount of soap used. 
B) the temperature of the water. 
C) the mechanical action of rubbing. 
D) the chemical activities in the soap. 
A) the amount of soap used. 
B) the temperature of the water. 
C) the mechanical action of rubbing. 
D) the chemical activities in the soap.

Question 10

Class I biological safety cabinets:

Accepted Answer

A) sterilize the air as it passes over the material in the cabinet. 
B) protect the environment from contamination by biological agents by filtering the air as it is exhausted from the cabinet. 
C) are completely enclosed and have gloves that enable the worker to manipulate the material in the cabinet. 
D) are often called laminar flow hoods. 
A) sterilize the air as it passes over the material in the cabinet. 
B) protect the environment from contamination by biological agents by filtering the air as it is exhausted from the cabinet. 
C) are completely enclosed and have gloves that enable the worker to manipulate the material in the cabinet. 
D) are often called laminar flow hoods.

Question 11

The most effective antiseptic agent against blood spills and therefore the antiseptic of choice in laboratories where these agents are likely found is:

Accepted Answer

A) 70% ethanol. 
B) phenol compound. 
C) absolute ethanol. 
D) 10% bleach. 
A) 70% ethanol. 
B) phenol compound. 
C) absolute ethanol. 
D) 10% bleach.

Question 12

Procedures such as grinding,mincing,vortexing,and preparing smears:

Accepted Answer

A) are hazardous and should be performed while wearing gloves and gown protection. 
B) create aerosol droplets and should be performed only in an approved biosafety cabinet. 
C) should be avoided with specimens known to contain infectious agents. 
D) produce a spill hazard and should be performed over an absorptive mat. 
A) are hazardous and should be performed while wearing gloves and gown protection. 
B) create aerosol droplets and should be performed only in an approved biosafety cabinet. 
C) should be avoided with specimens known to contain infectious agents. 
D) produce a spill hazard and should be performed over an absorptive mat.

Question 13

Biosafety Level 2 agents:

Accepted Answer

A) include those that are the common agents of infectious disease. 
B) require only standard good laboratory technique. 
C) include M.tuberculosis,M.gordonae,and Brucella. 
D) require the use of maximum containment facilities. 
A) include those that are the common agents of infectious disease. 
B) require only standard good laboratory technique. 
C) include M.tuberculosis,M.gordonae,and Brucella. 
D) require the use of maximum containment facilities.

Question 14

Optimal protection of specimen,personnel,and environment is accomplished with which class of biological safety cabinet?

Accepted Answer

A) Class I 
B) Class II 
C) Class III 
D) Class IV 
A) Class I 
B) Class II 
C) Class III 
D) Class IV

Question 15

Standard Precautions in the microbiology laboratory apply to:

Accepted Answer

A) all clinical specimens. 
B) clinical specimens that contain blood or body fluids. 
C) blood,body fluids,feces,sputum,saliva,urine,and vomitus. 
D) only specimens that are visibly bloody. 
A) all clinical specimens. 
B) clinical specimens that contain blood or body fluids. 
C) blood,body fluids,feces,sputum,saliva,urine,and vomitus. 
D) only specimens that are visibly bloody.

Question 16

Working with biosafety Level 3 agents requires:

Accepted Answer

A) U.S.Department of Agriculture (USD
A) credentials and a postgraduate degree in microbiology or a related field. 
B) laboratory design features that control air movement. 
C) personnel to wear protective clothing and devices and enter a decontaminating chamber before leaving. 
D) workers to have blood samples routinely tested to rule out infection or contamination. 
A) U.S.Department of Agriculture (USD
A) credentials and a postgraduate degree in microbiology or a related field. 
B) laboratory design features that control air movement. 
C) personnel to wear protective clothing and devices and enter a decontaminating chamber before leaving. 
D) workers to have blood samples routinely tested to rule out infection or contamination.

Question 17

To be sterilized in an autoclave,infectious medical waste should be:

Accepted Answer

A) packed tightly to avoid dangerous pressure pockets. 
B) processed for 15 to 20 minutes at 121°C and 30 pounds per square inch (psi)pressure. 
C) processed for 30 to 60 minutes at 132°C and 15 psi pressure. 
D) processed for at least 1 hour at 121°C and 15 psi pressure. 
A) packed tightly to avoid dangerous pressure pockets. 
B) processed for 15 to 20 minutes at 121°C and 30 pounds per square inch (psi)pressure. 
C) processed for 30 to 60 minutes at 132°C and 15 psi pressure. 
D) processed for at least 1 hour at 121°C and 15 psi pressure.

Exam 4: Laboratory Safety

Standard Precautions include:

A cleaning solution used on the venipuncture site to avoid skin contamination of the blood sample is called a(n):

Applying a chemical to the surface of the laboratory bench to remove the contaminating microorganisms is considered:

The agency that requires health care facilities to provide employees with all devices and mechanisms necessary to protect them from the hazards encountered in the laboratory is:

Information concerning a chemical product's health hazards,toxicity,and disposal procedures is:

Chemicals used for disinfection that are capable of disrupting cellular membranes and reduce the surface tension through cationic disruption of water molecules are termed:

Minimal protection of specimen,personnel,and environment is accomplished with which class of biological safety cabinet?

Biosafety Level 1 agents:

The primary action in hand washing that prevents the distribution or transmission of microorganisms is:

Class I biological safety cabinets:

The most effective antiseptic agent against blood spills and therefore the antiseptic of choice in laboratories where these agents are likely found is:

Procedures such as grinding,mincing,vortexing,and preparing smears:

Biosafety Level 2 agents:

Optimal protection of specimen,personnel,and environment is accomplished with which class of biological safety cabinet?

Standard Precautions in the microbiology laboratory apply to:

Working with biosafety Level 3 agents requires:

To be sterilized in an autoclave,infectious medical waste should be:

Microbial Taxonomy

Bacterial Genetics, metabolism, and Structure

Host-Microorganism Interactions

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Overview of Immunochemical Methods Used for Organism Detection

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Overview of the Methods and Strategies in Parasitology

Intestinal Protozoa

Blood and Tissue Protozoa

Protozoa From Other Body Sites

Intestinal Nematodes

Tissue Nematodes

Blood Nematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Zygomycetes, Dermatophytes, and Opportunistic and Systemic Mycoses

Dematiaceious Molds

Opportunistic Atypical Fungus: Pjirovecii

The Yeasts

Anti-fungal Susceptibility Testing, therapy, and Prevention

Overview of the Methods and Strategies in Virology