Question 1

The difference between polyvalent and monovalent antisera is that polyvalent antisera:

Accepted Answer

A)  confirm the presence of major factors. 
B)  confirm the presence or absence of factors. 
C)  identify only corresponding proteins. 
D)  both a and b 
A)  confirm the presence of major factors. 
B)  confirm the presence or absence of factors. 
C)  identify only corresponding proteins. 
D)  both a and b

Question 2

Immunoelectrophoresis (IEP)can divide the proteins of normal serum into _______________ distinct precipitation bands.

Accepted Answer

A)  5 to 10 
B)  15 to 20 
C)  25 to 40 
D)  45 to 100 
A)  5 to 10 
B)  15 to 20 
C)  25 to 40 
D)  45 to 100

Question 3

Immunoelectrophoresis (IEP)is a reliable and accurate method for detecting:

Accepted Answer

A)  structural abnormalities. 
B)  concentration changes in proteins. 
C)  concentration changes in lipids. 
D)  both a and b 
A)  structural abnormalities. 
B)  concentration changes in proteins. 
C)  concentration changes in lipids. 
D)  both a and b

Question 4

Immunofixation electrophoresis (IFE)should be reserved for anomalous proteins that are difficult to characterize by immunoelectrophoresis (IEP).

Accepted Answer

A) True 
 B)False

Question 5

Match the steps in the immunofixation electrophoresis (IFE)procedure with the appropriate description.
-Second step

Accepted Answer

A) Proteins are separated by their net charge. 
B) Polyspecific antisera are applied to 10 electrophoretic patterns. 
C) Protein reference patterns are stained and examined. 
D) Monospecific antisera are applied to five electrophoretic patterns. 
A) Proteins are separated by their net charge. 
B) Polyspecific antisera are applied to 10 electrophoretic patterns. 
C) Protein reference patterns are stained and examined. 
D) Monospecific antisera are applied to five electrophoretic patterns.

Question 6

Match the steps in the immunofixation electrophoresis (IFE)procedure with the appropriate description.
-First step

Accepted Answer

A) Proteins are separated by their net charge. 
B) Polyspecific antisera are applied to 10 electrophoretic patterns. 
C) Protein reference patterns are stained and examined. 
D) Monospecific antisera are applied to five electrophoretic patterns. 
A) Proteins are separated by their net charge. 
B) Polyspecific antisera are applied to 10 electrophoretic patterns. 
C) Protein reference patterns are stained and examined. 
D) Monospecific antisera are applied to five electrophoretic patterns.

Question 7

The primary application of immunofixation electrophoresis (IFE)is for the:

Accepted Answer

A)  quantitation of immunoglobulins. 
B)  separation of the five serum protein fractions. 
C)  characterization of monoclonal immunoglobulins. 
D)  all of the above. 
A)  quantitation of immunoglobulins. 
B)  separation of the five serum protein fractions. 
C)  characterization of monoclonal immunoglobulins. 
D)  all of the above.

Question 8

Immunofixation electrophoresis (IFE)can be optimized to give greater sensitivity and higher resolution than immunoelectrophoresis (IEP).

Accepted Answer

A) True 
 B)False

Question 9

Monoclonal immunoglobulin A (IgA)gammopathy has a distinctive immunoelectrophoretic feature of:

Accepted Answer

A)  antibody in the position of band precipitin. 
B)  abnormal curvature of precipitin band. 
C)  skewed arcs of a circle. 
D)  thickening and elongation of the precipitation band. 
A)  antibody in the position of band precipitin. 
B)  abnormal curvature of precipitin band. 
C)  skewed arcs of a circle. 
D)  thickening and elongation of the precipitation band.

Question 10

A monoclonal gammopathy is a:

Accepted Answer

A)  frequently diagnosed condition using immunoelectrophoresis (IEP). 
B)  condition in which a single clone of plasma cells produces increased levels of a single class of immunoglobulin. 
C)  condition in which a single clone of plasma cells produces increased levels of a single type of immunoglobulin. 
D)  all of the above. 
A)  frequently diagnosed condition using immunoelectrophoresis (IEP). 
B)  condition in which a single clone of plasma cells produces increased levels of a single class of immunoglobulin. 
C)  condition in which a single clone of plasma cells produces increased levels of a single type of immunoglobulin. 
D)  all of the above.

Exam 11: Electrophoresis Techniques

The difference between polyvalent and monovalent antisera is that polyvalent antisera:

Immunoelectrophoresis (IEP)can divide the proteins of normal serum into _______________ distinct precipitation bands.

Immunoelectrophoresis (IEP)is a reliable and accurate method for detecting:

Immunofixation electrophoresis (IFE)should be reserved for anomalous proteins that are difficult to characterize by immunoelectrophoresis (IEP).

Match the steps in the immunofixation electrophoresis (IFE)procedure with the appropriate description. -Second step

Match the steps in the immunofixation electrophoresis (IFE)procedure with the appropriate description. -First step

The primary application of immunofixation electrophoresis (IFE)is for the:

Immunofixation electrophoresis (IFE)can be optimized to give greater sensitivity and higher resolution than immunoelectrophoresis (IEP).

Monoclonal immunoglobulin A (IgA)gammopathy has a distinctive immunoelectrophoretic feature of:

A monoclonal gammopathy is a:

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Basic Serologic Laboratory Techniques

Point-Of-Care Testing

Agglutination Methods

Labeling Techniques in Immunoassay

Automated Procedures

Molecular Techniques

The Immune Response in Infectious Diseases

A Primer on Vaccines

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Acquired Immunodeficiency Syndrome

Hypersensitivity Reactions

Immunoproliferative Disorders

Autoimmune Disorders

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Solid Organ Transplantation

Bone Marrow Transplantation

Tumor Immunology

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