Question 1

The primer for DNA synthesis is an RNA molecule formed by the enzyme _______________.

Accepted Answer

primase

Question 2

DNA serves as the _____ for synthesis of complementary strands of DNA.

Accepted Answer

A)  primer 
B)  catalyst 
C)  template 
D)  primer and template 
E)  None of the answers is correct. 
A)  primer 
B)  catalyst 
C)  template 
D)  primer and template 
E)  None of the answers is correct. C

Question 3

The human genetic skin disease caused by a mutation in components of the human nucleotide-excision-repair pathway is called ___________________.

Accepted Answer

xeroderma pigmentosum

Question 4

The DNA strand that is replicated continuously is known as the _____________ strand.

Accepted Answer

The answer of The DNA strand that is replicated continuously...

Question 5

What type of topoisomerase does NOT require ATP?

Accepted Answer

A)  type I 
B)  type II 
C)  type III 
D)  type IV 
E)  All topoisomerases require ATP. 
A)  type I 
B)  type II 
C)  type III 
D)  type IV 
E)  All topoisomerases require ATP.

Question 6

What type of topoisomerase can introduce supercoils?

Accepted Answer

A)  type I 
B)  type II 
C)  type III 
D)  type IV 
E)  All topoisomerase can introduce supercoils. 
A)  type I 
B)  type II 
C)  type III 
D)  type IV 
E)  All topoisomerase can introduce supercoils.

Question 7

What must happen to the strands of DNA in order for replication to occur?

Accepted Answer

A)  supercoiling 
B)  annealing 
C)  melting 
D)  unwinding 
E)  None of the answers is correct. 
A)  supercoiling 
B)  annealing 
C)  melting 
D)  unwinding 
E)  None of the answers is correct.

Question 8

How are single-stranded regions of DNA maintained during polymerization?

Accepted Answer

Single-stranded bind

Question 9

Photolyase functions to

Accepted Answer

A)  repair pyrimidine dimers. 
B)  remove damaged bases. 
C)  ligate single-strand breaks. 
D)  remove 6-4 photoproducts. 
E)  None of the answers is correct. 
A)  repair pyrimidine dimers. 
B)  remove damaged bases. 
C)  ligate single-strand breaks. 
D)  remove 6-4 photoproducts. 
E)  None of the answers is correct.

Question 10

What proteins use ATP to separate DNA strands at specific sites?

Accepted Answer

A)  topoisomerases 
B)  single-stranded binding proteins 
C)  histones 
D)  gyrases 
E)  helicases 
A)  topoisomerases 
B)  single-stranded binding proteins 
C)  histones 
D)  gyrases 
E)  helicases

Question 11

What is a processive polymerase enzyme versus a distributive enzyme?

Accepted Answer

Processive enzymes catalyze mu

Question 12

What is the formula for the linking number?

Accepted Answer

A)  Lk = Wr − Tw 
B)  Lk = Wr + Tw 
C)  Tw = Wr + Lk 
D)  Lk = Tw/Wr 
E)  None of the answers is correct. 
A)  Lk = Wr − Tw 
B)  Lk = Wr + Tw 
C)  Tw = Wr + Lk 
D)  Lk = Tw/Wr 
E)  None of the answers is correct.

Question 13

What has been learned about ATP binding and hydrolysis in the helicase mechanism through the use of the non-hydrolyzable ATP analog,AMP-PNP?

Accepted Answer

Of the six helicase subunits,only four a

Question 14

Type ________ topoisomerase introduces negative supercoils in DNA while type ________ topoisomerase relaxes supercoil structures.

Accepted Answer

The answer of Type ________ topoisomerase introduces negative supercoils in...

Question 15

Which of the following best describes the directionality of DNA replication?

Accepted Answer

A)  5' → 3' direction on both strands 
B)  3' → 5' direction on both strands 
C)  5' → 3' direction on the leading strand, 3' → 5' on the lagging strand 
D)  3' → 5' direction on the leading strand, 5' → 3' on the lagging strand 
E)  None of the answers is correct. 
A)  5' → 3' direction on both strands 
B)  3' → 5' direction on both strands 
C)  5' → 3' direction on the leading strand, 3' → 5' on the lagging strand 
D)  3' → 5' direction on the leading strand, 5' → 3' on the lagging strand 
E)  None of the answers is correct.

Question 16

During prokaryotic DNA replication,the RNA primer pieces are removed by __________________.

Accepted Answer

The answer of During prokaryotic DNA replication,the RNA primer pieces...

Question 17

UV light causes damage to DNA by forming __________________.

Accepted Answer

The answer of UV light causes damage to DNA by...

Question 18

________________ are intermediates in recombination pathways composed of four polynucleotide chains in a cross-like structure.

Accepted Answer

The answer of ________________ are intermediates in recombination pathways composed...

Question 19

One function of the two metal cations that are required by DNA polymerases is

Accepted Answer

A)  to stabilize the negative charge of the transition state. 
B)  to bind ATP. 
C)  to complex with a Tyr residue in the active site. 
D)  to cleave improperly matched bases 
E)  None of the answers is correct. 
A)  to stabilize the negative charge of the transition state. 
B)  to bind ATP. 
C)  to complex with a Tyr residue in the active site. 
D)  to cleave improperly matched bases 
E)  None of the answers is correct.

Question 20

Topoisomerases are enzymes that introduce or eliminate supercoils in DNA during replication.What is the difference in mechanism and energy requirements for topoisomerase types I and II?

Accepted Answer

Type I topoisomerases catalyze the therm

Exam 28: DNA Replication, repair, and Recombination

The primer for DNA synthesis is an RNA molecule formed by the enzyme _______________.

DNA serves as the _____ for synthesis of complementary strands of DNA.

The human genetic skin disease caused by a mutation in components of the human nucleotide-excision-repair pathway is called ___________________.

The DNA strand that is replicated continuously is known as the _____________ strand.

What type of topoisomerase does NOT require ATP?

What type of topoisomerase can introduce supercoils?

What must happen to the strands of DNA in order for replication to occur?

How are single-stranded regions of DNA maintained during polymerization?

Photolyase functions to

What proteins use ATP to separate DNA strands at specific sites?

What is a processive polymerase enzyme versus a distributive enzyme?

What is the formula for the linking number?

What has been learned about ATP binding and hydrolysis in the helicase mechanism through the use of the non-hydrolyzable ATP analog,AMP-PNP?

Type ________ topoisomerase introduces negative supercoils in DNA while type ________ topoisomerase relaxes supercoil structures.

Which of the following best describes the directionality of DNA replication?

During prokaryotic DNA replication,the RNA primer pieces are removed by __________________.

UV light causes damage to DNA by forming __________________.

________________ are intermediates in recombination pathways composed of four polynucleotide chains in a cross-like structure.

One function of the two metal cations that are required by DNA polymerases is

Topoisomerases are enzymes that introduce or eliminate supercoils in DNA during replication.What is the difference in mechanism and energy requirements for topoisomerase types I and II?

Biochemistry: An Evolving Science

Protein Composition and Structure

Exploring Proteins and Proteomes

DNA, RNA, and the Flow of Genetic Information

Exploring Genes and Genomes

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

Oxidative Phosphorylation

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

RNA Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

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Type topoisomerase introduces negative supercoils in DNA while type topoisomerase relaxes supercoil structures.