Question 1

Which of the following DNA sequences contains a palindromic inverted repeat such as those recognized by restriction enzymes? (Note: Only one strand is shown.)

Accepted Answer

A)  CAGTCC 
B)  GCATCC 
C)  CGATTAGC 
D)  GAGAGAGA 
E)  GCATATGC 
A)  CAGTCC 
B)  GCATCC 
C)  CGATTAGC 
D)  GAGAGAGA 
E)  GCATATGC E

Question 2

What was the first free-living organism to have its genome sequenced?

Accepted Answer

A)  Homo sapiens 
B)  Escherichia coli 
C)  Drosophila melanogaster 
D)  Haemophilus influenzae 
E)  Canis domesticus 
A)  Homo sapiens 
B)  Escherichia coli 
C)  Drosophila melanogaster 
D)  Haemophilus influenzae 
E)  Canis domesticus D

Question 3

A number of tools are critical to gene exploration.Name at least four.

Accepted Answer

Tools include restriction enzyme analysis,blotting techniques (Southern and northern),DNA sequencing,solid-phase synthesis of nucleic acid,PCR,and computer analysis.

Question 4

Briefly outline how a cDNA library is made.

Accepted Answer

Reverse transcriptase is used to convert

Question 5

The Sanger technique for sequencing DNA involves the use of __________________ nucleotide analogs that terminate chain elongation.

Accepted Answer

2',3'-dide

Question 6

pUC plasmids are useful for screening cells that contain recombinant DNA because they contain the _____ gene.

Accepted Answer

A)  $\beta$-galactosidase 
B)  tetracycline resistance 
C)  green fluorescent protein 
D)  luciferase 
E)  None of the answers is correct. 
A)  $\beta$-galactosidase 
B)  tetracycline resistance 
C)  green fluorescent protein 
D)  luciferase 
E)  None of the answers is correct.

Question 7

How closely related are the human,rat,and puffer fish genomes?

Accepted Answer

Human and rat genome comparisons reveal

Question 8

A gene's function can be studied by inactivating the gene by a process known as gene disruption or _______________.

Accepted Answer

The answer of A gene's function can be studied by...

Question 9

cDNA attached to a microscope slide forms a _________________ used to study gene expression levels.

Accepted Answer

The answer of cDNA attached to a microscope slide forms...

Question 10

Who developed the polymerase chain reaction?

Accepted Answer

A)  Frederick Sanger 
B)  Kary Mullis 
C)  Edwin Southern 
D)  Paul Berg 
E)  Herbert Boyer 
A)  Frederick Sanger 
B)  Kary Mullis 
C)  Edwin Southern 
D)  Paul Berg 
E)  Herbert Boyer

Question 11

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is __________________.

Accepted Answer

The answer of The enzyme that catalyzes the formation of...

Question 12

How is gene disruption used to determine the function of a gene?

Accepted Answer

If a gene is completely disrupted (also

Question 13

Complementary,single-strand overhangs that are produced by some restriction endonucleases are referred to as ___________________.

Accepted Answer

sticky end

Question 14

_____ split(s)DNA into specific fragments.

Accepted Answer

A)  Plasmids 
B)  Reverse transcriptase 
C)  Polymerase chain reaction 
D)  Dideoxy nucleotide based sequencing 
E)  Restriction enzymes 
A)  Plasmids 
B)  Reverse transcriptase 
C)  Polymerase chain reaction 
D)  Dideoxy nucleotide based sequencing 
E)  Restriction enzymes

Question 15

PCR is the abbreviation for ____________________________,which is an in-vitro technique used to make multiple copies of a DNA molecule.

Accepted Answer

Polymerase

Question 16

Bacterial plasmid DNA and bacteriophage DNA are commonly used ______________ to introduce foreign DNA into a bacterium.

Accepted Answer

The answer of Bacterial plasmid DNA and bacteriophage DNA are...

Question 17

Each cycle in PCR includes

Accepted Answer

A)  DNA synthesis. 
B)  hybridization of primers. 
C)  strand separation. 
D)  restriction. 
E)  DNA synthesis, hybridization of primers, and strand separation. 
A)  DNA synthesis. 
B)  hybridization of primers. 
C)  strand separation. 
D)  restriction. 
E)  DNA synthesis, hybridization of primers, and strand separation.

Question 18

Explain the basis of the polymerase chain reaction.

Accepted Answer

Using primers that flank the sequence of

Question 19

How can DNA fragments of various sizes be separated?

Accepted Answer

Electrophoresis is used to separate DNA.

Question 20

The most common type of DNA sequencing is _____.

Accepted Answer

A)  polymerase chain reaction method 
B)  Sanger dideoxy method 
C)  reverse transcriptase method 
D)  transformation method 
E)  restriction method 
A)  polymerase chain reaction method 
B)  Sanger dideoxy method 
C)  reverse transcriptase method 
D)  transformation method 
E)  restriction method

Exam 5: Exploring Genes and Genomes

Which of the following DNA sequences contains a palindromic inverted repeat such as those recognized by restriction enzymes? (Note: Only one strand is shown.)

What was the first free-living organism to have its genome sequenced?

A number of tools are critical to gene exploration.Name at least four.

Briefly outline how a cDNA library is made.

The Sanger technique for sequencing DNA involves the use of __________________ nucleotide analogs that terminate chain elongation.

pUC plasmids are useful for screening cells that contain recombinant DNA because they contain the _____ gene.

How closely related are the human,rat,and puffer fish genomes?

A gene's function can be studied by inactivating the gene by a process known as gene disruption or _______________.

cDNA attached to a microscope slide forms a _________________ used to study gene expression levels.

Who developed the polymerase chain reaction?

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is __________________.

How is gene disruption used to determine the function of a gene?

Complementary,single-strand overhangs that are produced by some restriction endonucleases are referred to as ___________________.

_____ split(s)DNA into specific fragments.

PCR is the abbreviation for ____________________________,which is an in-vitro technique used to make multiple copies of a DNA molecule.

Bacterial plasmid DNA and bacteriophage DNA are commonly used ______________ to introduce foreign DNA into a bacterium.

Each cycle in PCR includes

Explain the basis of the polymerase chain reaction.

How can DNA fragments of various sizes be separated?

The most common type of DNA sequencing is _____.

Biochemistry: An Evolving Science

Protein Composition and Structure

Exploring Proteins and Proteomes

DNA, RNA, and the Flow of Genetic Information

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

Oxidative Phosphorylation

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, repair, and Recombination

RNA Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

Filters