Exam 8: Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

You homogenize a cell and isolate from it vesicles derived from the endoplasmic reticulum.When their biochemistry is analyzed,they are found to have the ability to synthesize testosterone.From what type of ER are they derived?

Which of the following carbohydrates is not synthesized in the Golgi complex?

What determines the specificity of vesicle fusion to a target membrane?

What are the differences between ribosomes that make secretory proteins and those that make proteins intended for the cytosol?

The coat of vesicles that transport materials around the cell interior ___________.

If you treated a macrophage with colchicine (a microtubular inhibitor),what would be likely to happen to the rate of phagocytosis? What would be likely to happen to the rate of phagocytosis if you treated the macrophage with an inhibitor of microfilament contractile activities?

How many subcompartments do peroxisomes have into which an imported protein can be placed?

A tissue that secretes a number of proteins is exposed in culture to radiolabeled amino acids for a very short period of time and then fixed immediately and prepared for autoradiography.What is seen after the autoradiograms are prepared?

Drugs that lower blood LDL levels are referred to as _______.

Why is RNAi now used as a strategy for investigating the effect of a missing protein more often than generating an organism that possesses a mutant gene?

Why does targeting glucocerebrosidase to lysosomes in macrophages serve as a treatment for Gaucher's disease?

What is the name for a brief incubation of a tissue with radioactivity during which labeled amino acids are incorporated into protein?

You are observing a cell process in which small vesicles continually merge with the cell membrane.A number of different treatments known to influence the secretion of specific materials seems to have no effect on the process.What type of secretion appears to be occurring?

What evidence suggests that the translocon,by itself,can properly orient transmembrane segments?

How do protein coats select the cargo molecules to be carried by the vesicles they help to form?

How many subcompartments are there in chloroplasts into which proteins can be delivered?

What specific cellular responses are known to be triggered by the regulated release of Ca²⁺ ions from the SER?

A protein that is normally secreted is genetically engineered by altering the gene that encodes it.The part of the gene encoding the KDEL sequence is removed from the C-terminus and two stop-transfer sequences are inserted in the middle of its gene.When the altered gene is reinserted into the cells,what happens?

What are the two sites within a cell at which protein synthesis is generally thought to occur?

The movement of vesicular-tubular carriers (VTCs)farther away from the ER and toward the Golgi complex occurs along tracks composed of what material?