Exam 8: Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

Which of the models below suggests that the Golgi cisternae are transient structures that form at the cis face of the stack by fusion of membranous carriers from the ER and ERGIC and that each cisterna travels through the Golgi complex from the cis to the trans end of the stack,changing in composition as it progresses?

A tissue has been briefly labeled with radiolabeled amino acids.It is then transferred to a medium containing unlabeled amino acids.This can be done several times with different tissue samples for varying periods of time.What is the entire procedure called?

Blöbel,Sabatini and Dobberstein proposed that the site of protein synthesis is determined by information contained in the N-terminal portion of the protein,the first part to emerge from the ribosome.What did they call their proposal?

Which pH below would be most likely to favor the operation of a lysosomal enzyme?

Vesicles that move through the Golgi complex from a trans-donor to a cis-acceptor membrane are said to move in a(n)__________ direction.

Based on what is known about the involvement of calcium ions in exocytosis,what should happen if Ca²⁺ ions are injected into a cell?

What kind of molecule has been implicated in preparing polypeptides for mitochondrial uptake,including those that specifically direct mitochondrial proteins to the cytosolic surface of the outer mitochondrial membrane?

Plant cells are exposed to an inhibitor of H⁺-ATPase in the tonoplast membrane.What will the immediate effect be?

To what residue of a polypeptide are N-linked oligosaccharide chains attached as that poypeptide enters the RER lumen through the translocon?

CDG1b results from a deficiency in what enzyme?

What drives the engulfment of particulate material by phagocytosis?

The drugs that lower LDL concentration in the blood function by ________.

Studies of cell physiology that occur in test tubes that do not contain whole cells are called ______.

What is the effect on a yeast cell of the presence of a mutant gene involved in vesicle fusion?

Why does the cell use proteasomes to destroy misfolded proteins?

To what site does Sar1 bind after it binds to GTP?

Treatment of lysosomal storage diseases with enzyme replacement therapy involves _______.

Which endosomes are typically located near the peripheral region of the cell?

The uptake of relatively large particulate matter into the cell is called _________.

SNAREs vary in structure quite a bit,but all of them contain a common domain.Where is this domain located,of what is it composed and what is it called?