Exam 8: Enzymes: Basic Concepts and Kinetics

Explore all topics

The difference between the standard-state free energy, ΔGº, and the biochemical standard-state free energy is that ΔGº′ refers to the standard free-energy change at ________.

Free

(Short Answer)

4.7/5

(25)

Question 1

Correct Answer:

Verified

pH 7

What is an affinity label?

Free

(Essay)

4.9/5

(40)

Question 2

Correct Answer:

Verified

This is a substrate analog that is structurally similar to the substrate, binds to the active site, and chemically reacts with a residue in the active site. It is used to study enzyme structure and mechanism.

What term describes an enzyme without its required cofactor?

Free

(Multiple Choice)

4.8/5

(34)

Question 3

Correct Answer:

Verified

There are six basic categories of enzymes. List the categories, and define the type of reaction.

(Essay)

4.9/5

(39)

Question 4

What is the common strategy by which catalysis occurs?

(Multiple Choice)

4.8/5

(41)

Question 5

If a reaction is exergonic at specific reactant concentrations, it is considered _____.

(Multiple Choice)

4.9/5

(37)

Question 6

In this type of inhibition, the inhibitor can only bind to the ES complex to form an ESI complex.

(Multiple Choice)

4.8/5

(41)

Question 7

Five K_M values are given for the binding of substrates to a particular enzyme. Which has the strongest affinity when k ₁ is greater than k₂?

(Multiple Choice)

4.9/5

(37)

Question 8

The k_cat is often referred to as the _____.

(Multiple Choice)

4.9/5

(38)

Question 9

The Circe effect enhances what aspect of enzyme catalysis?

(Multiple Choice)

4.8/5

(34)

Question 10

In many enzyme assays, the natural substrate and product are not used. Why?

(Essay)

4.9/5

(29)

Question 11

Explain what an abzyme is and give an example.

(Essay)

4.9/5

(29)

Question 12

Which of the following is true under the following conditions: the enzyme concentration is 5 nM, the substrate concentration is 5 mM, and the K_M is 5 $\mu$ 0M?

(Multiple Choice)

4.9/5

(27)

Question 13

A(n) _____ requires an input of energy to proceed.

(Multiple Choice)

4.8/5

(27)

Question 14

You believe a substrate fits rigidly into a cleft like a key into a lock, but your roommate believes that the structure of the enzyme adapts to the substrate. Who is right?

(Essay)

4.9/5

(42)

Question 15

Give an example of a reaction catalyzed by a hydrolase.

(Essay)

4.8/5

(37)

Question 16

What term is used to describe the dynamic recognition of the substrate when binding to an enzyme occurs?

(Multiple Choice)

4.8/5

(39)

Question 17

Most known enzymes are proteins; however, some ___________ molecules have been shown to possess catalytic activity.

(Short Answer)

4.8/5

(27)

Question 18

What conclusion can be drawn concerning an inhibitor if the V_max is the same in the presence and absence of the inhibitor?

(Multiple Choice)

4.8/5

(45)

Question 19

Compounds that resemble the transition state of a catalyzed reaction and inhibit enzyme activity are called ____________________________.

(Short Answer)

4.7/5

(34)

Question 20

Showing 1 - 20 of 50

Exam 8: Enzymes: Basic Concepts and Kinetics

The difference between the standard-state free energy, ΔGº, and the biochemical standard-state free energy is that ΔGº′ refers to the standard free-energy change at ________.

What is an affinity label?

What term describes an enzyme without its required cofactor?

There are six basic categories of enzymes. List the categories, and define the type of reaction.

What is the common strategy by which catalysis occurs?

If a reaction is exergonic at specific reactant concentrations, it is considered _____.

In this type of inhibition, the inhibitor can only bind to the ES complex to form an ESI complex.

Five KM values are given for the binding of substrates to a particular enzyme. Which has the strongest affinity when k 1 is greater than k2?

The kcat is often referred to as the _____.

The Circe effect enhances what aspect of enzyme catalysis?

In many enzyme assays, the natural substrate and product are not used. Why?

Explain what an abzyme is and give an example.

Which of the following is true under the following conditions: the enzyme concentration is 5 nM, the substrate concentration is 5 mM, and the KM is 5 μ\muμ 0M?

A(n) _____ requires an input of energy to proceed.

You believe a substrate fits rigidly into a cleft like a key into a lock, but your roommate believes that the structure of the enzyme adapts to the substrate. Who is right?

Give an example of a reaction catalyzed by a hydrolase.

What term is used to describe the dynamic recognition of the substrate when binding to an enzyme occurs?

Most known enzymes are proteins; however, some ___________ molecules have been shown to possess catalytic activity.

What conclusion can be drawn concerning an inhibitor if the Vmax is the same in the presence and absence of the inhibitor?

Compounds that resemble the transition state of a catalyzed reaction and inhibit enzyme activity are called ____________________________.

Biochemistry: an Evolving Science

Protein Composition and Structure

Exploring Proteins and Proteomes

DNA, RNA, and the Flow of Genetic Information

Exploring Genes and Genomes

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Oxidative Phosphorylation

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, Repair, and Recombination

Rna Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

Filters

Five K_M values are given for the binding of substrates to a particular enzyme. Which has the strongest affinity when k ₁ is greater than k₂?

The k_cat is often referred to as the _____.

Which of the following is true under the following conditions: the enzyme concentration is 5 nM, the substrate concentration is 5 mM, and the K_M is 5 $\mu$ 0M?

What conclusion can be drawn concerning an inhibitor if the V_max is the same in the presence and absence of the inhibitor?