Question 1

PCR is the abbreviation for ____________________________, which is an in-vitro technique used to make multiple copies of a DNA molecule.

Accepted Answer

Polymerase Chain Reaction

Question 2

Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as ___________________.

Accepted Answer

sticky ends or cohesive ends

Question 3

If a gene is inserted into the gene that encodes an antibiotic resistance marker, will the resulting clone be sensitive or resistant to the antibiotic?

Accepted Answer

When inserted into a gene, the new DNA interrupts the previous gene. Thus, the antibiotic gene is unlikely to be functional, the clone will be sensitive to the antibiotic, and the clone will not grow.

Question 4

How is a single gene of interest identified on a plate containing many different library clones?

Accepted Answer

By using a probe specific for the DNA of

Question 5

What enzyme is used to create DNA from RNA?

Accepted Answer

A)  RNA polymerase 
B)  DNA polymerase 
C)  reverse transcriptase 
D)  RNA-DNA transcriptase 
E)  None of the answers is correct. 
A)  RNA polymerase 
B)  DNA polymerase 
C)  reverse transcriptase 
D)  RNA-DNA transcriptase 
E)  None of the answers is correct.

Question 6

How can DNA fragments of various sizes be separated?

Accepted Answer

Electrophoresis is used to separate DNA.

Question 7

cDNA attached to a microscope slide forms a _________________ used to study gene expression levels.

Accepted Answer

The answer of cDNA attached to a microscope slide forms...

Question 8

A polylinker site contains

Accepted Answer

A)  many closely spaced restriction enzyme sites. 
B)  links for antibiotic resistance. 
C)  sequences allowing linkage to mRNA. 
D)  a sequence that allows for insertion into the host chromosome. 
E)  None of the answers is correct. 
A)  many closely spaced restriction enzyme sites. 
B)  links for antibiotic resistance. 
C)  sequences allowing linkage to mRNA. 
D)  a sequence that allows for insertion into the host chromosome. 
E)  None of the answers is correct.

Question 9

Current DNA sequencing commonly uses _____ base analogues.

Accepted Answer

A)  fluorescent 
B)  radioactive 
C)  phosphorescent 
D)  cross-linked 
E)  photoreactive 
A)  fluorescent 
B)  radioactive 
C)  phosphorescent 
D)  cross-linked 
E)  photoreactive

Question 10

What is a DNA probe?

Accepted Answer

A DNA probe is used to identify specific

Question 11

A plasmid is an example of a common ______.

Accepted Answer

A)  cosmid 
B)  chromosome 
C)  gene 
D)  vector 
E)  None of the answers is correct. 
A)  cosmid 
B)  chromosome 
C)  gene 
D)  vector 
E)  None of the answers is correct.

Question 12

How is gene disruption used to determine the function of a gene?

Accepted Answer

If a gene is completely disrupted (also

Question 13

The Sanger technique for sequencing DNA involves the use of __________________ nucleotide analogs that terminate chain elongation.

Accepted Answer

2',3'-dide

Question 14

A gene's function can be studied by inactivating the gene by a process known as gene disruption or _______________.

Accepted Answer

The answer of A gene's function can be studied by...

Question 15

How closely related are the human, rat, and puffer fish genomes?

Accepted Answer

Human and rat genome comparisons reveal

Question 16

Explain the basis of the Sanger method.

Accepted Answer

Sanger sequencing is based upon replicat

Question 17

Briefly outline how a cDNA library is made.

Accepted Answer

Reverse transcriptase is used to convert

Question 18

Which of the following DNA sequences contains a palindromic inverted repeat such as those recognized by restriction enzymes? (Note: Only one strand is shown.)

Accepted Answer

A)  CAGTCC 
B)  GCATCC 
C)  CGATTAGC 
D)  GAGAGAGA 
E)  GCATATGC 
A)  CAGTCC 
B)  GCATCC 
C)  CGATTAGC 
D)  GAGAGAGA 
E)  GCATATGC

Question 19

Animals that harbor a foreign gene as a result of recombinant gene manipulation are called

Accepted Answer

A)  transgenic. 
B)  mutants. 
C)  aliens. 
D)  All of the answers are correct. 
E)  None of the answers is correct. 
A)  transgenic. 
B)  mutants. 
C)  aliens. 
D)  All of the answers are correct. 
E)  None of the answers is correct.

Question 20

Describe two ways PCR can be used in medical diagnosis.

Accepted Answer

PCR can be used to identify the presence

Exam 5: Exploring Genes and Genomes

PCR is the abbreviation for ____________________________, which is an in-vitro technique used to make multiple copies of a DNA molecule.

Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as ___________________.

If a gene is inserted into the gene that encodes an antibiotic resistance marker, will the resulting clone be sensitive or resistant to the antibiotic?

How is a single gene of interest identified on a plate containing many different library clones?

What enzyme is used to create DNA from RNA?

How can DNA fragments of various sizes be separated?

cDNA attached to a microscope slide forms a _________________ used to study gene expression levels.

A polylinker site contains

Current DNA sequencing commonly uses _____ base analogues.

What is a DNA probe?

A plasmid is an example of a common ______.

How is gene disruption used to determine the function of a gene?

The Sanger technique for sequencing DNA involves the use of __________________ nucleotide analogs that terminate chain elongation.

A gene's function can be studied by inactivating the gene by a process known as gene disruption or _______________.

How closely related are the human, rat, and puffer fish genomes?

Explain the basis of the Sanger method.

Briefly outline how a cDNA library is made.

Which of the following DNA sequences contains a palindromic inverted repeat such as those recognized by restriction enzymes? (Note: Only one strand is shown.)

Animals that harbor a foreign gene as a result of recombinant gene manipulation are called

Describe two ways PCR can be used in medical diagnosis.

Biochemistry: an Evolving Science

Protein Composition and Structure

Exploring Proteins and Proteomes

DNA, RNA, and the Flow of Genetic Information

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Oxidative Phosphorylation

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, Repair, and Recombination

Rna Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

Filters