Question 1

Who developed the polymerase chain reaction?

Accepted Answer

A)  Frederick Sanger 
B)  Kary Mullis 
C)  Edwin Southern 
D)  Paul Berg 
E)  Herbert Boyer 
A)  Frederick Sanger 
B)  Kary Mullis 
C)  Edwin Southern 
D)  Paul Berg 
E)  Herbert Boyer

Question 2

What advantage can be gained by splicing together portions of two different genes?

Accepted Answer

This technique allows the creation of no

Question 3

The enzyme _________________ can be used to add nucleotides to the 3' end of DNA.

Accepted Answer

terminal t

Question 4

What was the first free-living organism to have its genome sequenced?

Accepted Answer

A)  Homo sapiens 
B)  Escherichia coli 
C)  Drosophila melanogaster 
D)  Haemophilus influenzae 
E)  Canis domesticus 
A)  Homo sapiens 
B)  Escherichia coli 
C)  Drosophila melanogaster 
D)  Haemophilus influenzae 
E)  Canis domesticus

Question 5

Comparative genomics involves

Accepted Answer

A)  looking for similarities in complementary strands of DNA. 
B)  using sequence information to compare human DNA with that from other organisms. 
C)  comparing protein expressed in different cell types. 
D)  comparing mitochondrial versus nuclear DNA. 
E)  None of the answers is correct. 
A)  looking for similarities in complementary strands of DNA. 
B)  using sequence information to compare human DNA with that from other organisms. 
C)  comparing protein expressed in different cell types. 
D)  comparing mitochondrial versus nuclear DNA. 
E)  None of the answers is correct.

Question 6

In solid-phase synthesis of oligonucleotides, the 2'deoxyribonucleotide-3'-phosphoramidite is added to the _______ end of the growing oligonucleotide.

Accepted Answer

The answer of In solid-phase synthesis of oligonucleotides, the 2'deoxyribonucleotide-3'-phosphoramidite...

Question 7

Plasmids used in recombinant DNA technology typically

Accepted Answer

A)  possess a gene for antibiotic resistance. 
B)  replicate independently of the host genome. 
C)  are circular double-stranded molecules. 
D)  contain reporter genes. 
E)  All of the answers are correct. 
A)  possess a gene for antibiotic resistance. 
B)  replicate independently of the host genome. 
C)  are circular double-stranded molecules. 
D)  contain reporter genes. 
E)  All of the answers are correct.

Question 8

Design a potential DNA-restriction enzyme site. Show both strands.

Accepted Answer

Any palindromic (inv

Question 9

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is __________________.

Accepted Answer

The answer of The enzyme that catalyzes the formation of...

Question 10

___________________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

Accepted Answer

Restriction endonucl

Question 11

The probe used to isolate a gene from a genomic library is often

Accepted Answer

A)  the ligand that binds to the protein. 
B)  its promoter region. 
C)  a portion of the mRNA of the gene. 
D)  the protein produced by the gene. 
E)  None of the answers is correct. 
A)  the ligand that binds to the protein. 
B)  its promoter region. 
C)  a portion of the mRNA of the gene. 
D)  the protein produced by the gene. 
E)  None of the answers is correct.

Question 12

_____ split(s) DNA into specific fragments.

Accepted Answer

A)  Plasmids 
B)  Reverse transcriptase 
C)  Polymerase chain reaction 
D)  Dideoxy nucleotide based sequencing 
E)  Restriction enzymes 
A)  Plasmids 
B)  Reverse transcriptase 
C)  Polymerase chain reaction 
D)  Dideoxy nucleotide based sequencing 
E)  Restriction enzymes

Question 13

Reverse transcriptase is normally encoded in DNA from

Accepted Answer

A)  plants. 
B)  retroviruses. 
C)  mitochondria. 
D)  archaea. 
E)  None of the answers is correct. 
A)  plants. 
B)  retroviruses. 
C)  mitochondria. 
D)  archaea. 
E)  None of the answers is correct.

Question 14

The first three bases of the six-base recognition cleavage site of HindIII are AAG. What is the complete sequence of this 6 bp site?

Accepted Answer

A)  AAGAAG 
B)  AAGCTT 
C)  AAGGAA 
D)  AAGCUU 
E)  None of the answers is correct. 
A)  AAGAAG 
B)  AAGCTT 
C)  AAGGAA 
D)  AAGCUU 
E)  None of the answers is correct.

Question 15

A number of tools are critical to gene exploration. Name at least four.

Accepted Answer

Tools include restriction enzy

Question 16

How is a gene gun used?

Accepted Answer

DNA is coated onto tungsten pe

Question 17

Why are Met and Trp codons often used to design DNA probes from amino acid sequences?

Accepted Answer

A)  They are not degenerate and have single codons. 
B)  Met is the first amino acid in the protein chain. 
C)  Both are used often in proteins. 
D)  They always occur together in protein sequences. 
E)  None of the answers is correct. 
A)  They are not degenerate and have single codons. 
B)  Met is the first amino acid in the protein chain. 
C)  Both are used often in proteins. 
D)  They always occur together in protein sequences. 
E)  None of the answers is correct.

Question 18

Techniques for engineering new proteins by site-directed gene mutations include

Accepted Answer

A)  oligonucleotide directed mutagenesis. 
B)  cassette mutagenesis. 
C)  chromosome walking mutagenesis. 
D)  oligonucleotide directed mutagenesis and cassette mutagenesis. 
E)  All of the answers are correct. 
A)  oligonucleotide directed mutagenesis. 
B)  cassette mutagenesis. 
C)  chromosome walking mutagenesis. 
D)  oligonucleotide directed mutagenesis and cassette mutagenesis. 
E)  All of the answers are correct.

Question 19

The most common type of DNA sequencing is _____.

Accepted Answer

A)  polymerase chain reaction method 
B)  Sanger dideoxy method 
C)  reverse transcriptase method 
D)  transformation method 
E)  restriction method 
A)  polymerase chain reaction method 
B)  Sanger dideoxy method 
C)  reverse transcriptase method 
D)  transformation method 
E)  restriction method

Question 20

Genes can be inserted into eukaryotic cells by

Accepted Answer

A)  viruses. 
B)  plasmids. 
C)  microinjection. 
D)  All of the answers are correct. 
E)  None of the answers is correct. 
A)  viruses. 
B)  plasmids. 
C)  microinjection. 
D)  All of the answers are correct. 
E)  None of the answers is correct.

Exam 5: Exploring Genes and Genomes

Who developed the polymerase chain reaction?

What advantage can be gained by splicing together portions of two different genes?

The enzyme _________________ can be used to add nucleotides to the 3' end of DNA.

What was the first free-living organism to have its genome sequenced?

Comparative genomics involves

In solid-phase synthesis of oligonucleotides, the 2'deoxyribonucleotide-3'-phosphoramidite is added to the _______ end of the growing oligonucleotide.

Plasmids used in recombinant DNA technology typically

Design a potential DNA-restriction enzyme site. Show both strands.

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is __________________.

___________________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

The probe used to isolate a gene from a genomic library is often

_____ split(s) DNA into specific fragments.

Reverse transcriptase is normally encoded in DNA from

The first three bases of the six-base recognition cleavage site of HindIII are AAG. What is the complete sequence of this 6 bp site?

A number of tools are critical to gene exploration. Name at least four.

How is a gene gun used?

Why are Met and Trp codons often used to design DNA probes from amino acid sequences?

Techniques for engineering new proteins by site-directed gene mutations include

The most common type of DNA sequencing is _____.

Genes can be inserted into eukaryotic cells by

Biochemistry: an Evolving Science

Protein Composition and Structure

Exploring Proteins and Proteomes

DNA, RNA, and the Flow of Genetic Information

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Oxidative Phosphorylation

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, Repair, and Recombination

Rna Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

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