Question 1

What are some of the advantages and drawbacks of NMR spectroscopy compared to x-ray crystallography?

Accepted Answer

While both are expensive and time-consum

Question 2

An ELISA can be used for

Accepted Answer

A)  quantitative analysis. 
B)  size analysis. 
C)  protein sequencing 
D)  All of the answers are correct. 
E)  None of the answers is correct. 
A)  quantitative analysis. 
B)  size analysis. 
C)  protein sequencing 
D)  All of the answers are correct. 
E)  None of the answers is correct.

Question 3

How can recombinant DNA technology aid in protein purification?

Accepted Answer

Large quantities of proteins can be expr

Question 4

How can a protein's isoelectric point be used in protein purification (i.e., isoelectric focusing)?

Accepted Answer

Isoelectric focusing is an electrophoret

Question 5

After a protein is purified, what is the next step in determining the structure of a protein by x-ray crystallography?

Accepted Answer

The next step is to prepare a

Question 6

Another name for an antigenic determinant is _____.

Accepted Answer

A)  epitope 
B)  epimer 
C)  epinephrine 
D)  epidemic 
E)  None of the answers is correct. 
A)  epitope 
B)  epimer 
C)  epinephrine 
D)  epidemic 
E)  None of the answers is correct.

Question 7

The mobility of proteins in SDS-PAGE is inversely proportional to the _____________.

Accepted Answer

logarithm of their m

Question 8

Briefly describe how an ELISA works.

Accepted Answer

In ELISA, the antigen of interest is com

Question 9

Two-dimensional electrophoresis is a combination of what two techniques?

Accepted Answer

A)  ion-exchange chromatography and affinity chromatography 
B)  ion-exchange chromatography and SDS-PAGE 
C)  SDS-PAGE and affinity chromatography 
D)  isoelectric focusing and SDS-PAGE 
E)  isoelectric focusing and ion-exchange chromatography 
A)  ion-exchange chromatography and affinity chromatography 
B)  ion-exchange chromatography and SDS-PAGE 
C)  SDS-PAGE and affinity chromatography 
D)  isoelectric focusing and SDS-PAGE 
E)  isoelectric focusing and ion-exchange chromatography

Question 10

How is lactate dehydrogenase assayed?

Accepted Answer

It is assayed by the increase

Exam 3: Exploring Proteins and Proteomes

What are some of the advantages and drawbacks of NMR spectroscopy compared to x-ray crystallography?

An ELISA can be used for

How can recombinant DNA technology aid in protein purification?

How can a protein's isoelectric point be used in protein purification (i.e., isoelectric focusing)?

After a protein is purified, what is the next step in determining the structure of a protein by x-ray crystallography?

Another name for an antigenic determinant is _____.

The mobility of proteins in SDS-PAGE is inversely proportional to the _____________.

Briefly describe how an ELISA works.

Two-dimensional electrophoresis is a combination of what two techniques?

How is lactate dehydrogenase assayed?

Biochemistry: an Evolving Science

Protein Composition and Structure

DNA, RNA, and the Flow of Genetic Information

Exploring Genes and Genomes

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Oxidative Phosphorylation

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, Repair, and Recombination

Rna Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

Filters