Question 1

Lysogeny is essential for the virulence of many pathogenic bacterial strains.

Accepted Answer

A) True 
 B)False

Question 2

Compare and contrast generalized transduction with specialized transduction.

Accepted Answer

Transduction as a whole is the transfer

Question 3

The use of transposons to generate mutations is a convenient way to create bacterial mutations in the laboratory.

Accepted Answer

A) True 
 B)False

Question 4

Toxigenicity in Corynebacterium diphtheriae is due to phage conversion.

Accepted Answer

A) True 
 B)False

Question 5

If a bacterium carrying a plasmid that confers resistance to ampicillin is placed into medium without ampicillin,it may

Accepted Answer

A) gain resistance to other antibiotics. 
B) transfer resistance to other cultures in the laboratory. 
C) undergo a reversion mutation. 
D) lose the plasmid because there is no selection for ampicillin resistance. 
A) gain resistance to other antibiotics. 
B) transfer resistance to other cultures in the laboratory. 
C) undergo a reversion mutation. 
D) lose the plasmid because there is no selection for ampicillin resistance.

Question 6

When UV radiation damage occurs,DNA repair occurs only in the absence of template instruction.

Accepted Answer

A) True 
 B)False

Question 7

Homologous recombination has been observed in

Accepted Answer

A) Archaea. 
B) Bacteria. 
C) Eukarya. 
D) Archaea,Bacteria,and Eukarya. 
A) Archaea. 
B) Bacteria. 
C) Eukarya. 
D) Archaea,Bacteria,and Eukarya.

Question 8

The designations Phe⁻,Leu⁻,and Ser⁺ refer to an organism's

Accepted Answer

A) plasmid type. 
B) genotype. 
C) phenotype. 
D) mutation type. 
A) plasmid type. 
B) genotype. 
C) phenotype. 
D) mutation type.

Question 9

Which process listed below does NOT require homologous recombination to maintain the new genetic material in the recipient cell?

Accepted Answer

A) conjugation of an F+ plasmid 
B) gene transfer through a genetic transfer agent 
C) transduction by a dsDNA phage Mu 
D) transformation of a linear piece of DNA 
A) conjugation of an F+ plasmid 
B) gene transfer through a genetic transfer agent 
C) transduction by a dsDNA phage Mu 
D) transformation of a linear piece of DNA

Question 10

You have performed the following mating experiment using Hfr and F⁻ strains of Escherichia coli:
 Hfr (thr⁺ leu⁺ gal⁺ strˢ)× F⁻ (thr⁻ leu⁻ gal⁻ strʳ).Which of the following selective media would you use to score recombinant colonies?

Accepted Answer

A) minimal medium 
B) minimal medium + streptomycin 
C) minimal medium + threonine 
D) minimal medium + streptomycin + threonine 
A) minimal medium 
B) minimal medium + streptomycin 
C) minimal medium + threonine 
D) minimal medium + streptomycin + threonine

Question 11

How might mutations in the   sequence,whose enzyme is involved in DNA proofreading,be beneficial for a microbe in a highly competitive and dynamic community?

Accepted Answer

Mutations in @#IMG-DLM& would result in an increas

Question 12

Which of the following methods may introduce foreign DNA into a recipient?

Accepted Answer

A) transformation 
B) transduction 
C) conjugation 
D) transformation,transduction,and conjugation 
A) transformation 
B) transduction 
C) conjugation 
D) transformation,transduction,and conjugation

Question 13

Which of the following features are common to transformation,transduction,and conjugation? (1)unidirectional transfer of genes
(2)incomplete gene transfer
(3)homologous recombination
(4)meiosis occurring in the recipient

Accepted Answer

A) 1,2,3 
B) 1,2 
C) 3,4 
D) 1,2,4 
A) 1,2,3 
B) 1,2 
C) 3,4 
D) 1,2,4

Question 14

A mutant you made in the laboratory with 5-bromouracil suddenly regains the wild-type phenotype.You discuss this phenomenon with your advisor and colleagues,and they suggest you try transposon mutagenesis to avoid this problem and create stable mutants.Why would mutants created with 5-bromouracil be more likely to regain the wild-type phenotype than mutants created via transposon mutagenesis?

Accepted Answer

Because a point mutation involves only a

Question 15

The positions of genes on a genetic map are marked according to

Accepted Answer

A) degrees. 
B) nanometers. 
C) radians. 
D) minutes. 
A) degrees. 
B) nanometers. 
C) radians. 
D) minutes.

Question 16

A typical mutation rate for a bacterium is in the range of 10⁻⁶ to 10⁻⁹ per kbp.

Accepted Answer

A) True 
 B)False

Question 17

Consider the following experiment.First,large populations of two mutant strains of Escherichia coli are mixed,each requiring a different,single amino acid.After plating them onto a minimal medium,45 colonies grew.Which of the following may explain this result?

Accepted Answer

A) The colonies may be due to back mutation (reversion). 
B) The colonies may be due to recombination. 
C) Either A or B is possible. 
D) Neither A nor B is possible. 
A) The colonies may be due to back mutation (reversion). 
B) The colonies may be due to recombination. 
C) Either A or B is possible. 
D) Neither A nor B is possible.

Question 18

The evolutionary significance of phage conversion likely stems from an effective alteration of host cells.

Accepted Answer

A) True 
 B)False

Question 19

The enzyme transposase may be coded for by insertion sequences on a

Accepted Answer

A) chromosome. 
B) phage. 
C) plasmid. 
D) chromosome,phage,or plasmid. 
A) chromosome. 
B) phage. 
C) plasmid. 
D) chromosome,phage,or plasmid.

Question 20

Plasmids that govern their own transfer are known as

Accepted Answer

A) transformable. 
B) transmutable. 
C) conjugative. 
D) transfective. 
A) transformable. 
B) transmutable. 
C) conjugative. 
D) transfective.

Exam 10: Genetics of Bacteria and Archaea

Lysogeny is essential for the virulence of many pathogenic bacterial strains.

Compare and contrast generalized transduction with specialized transduction.

The use of transposons to generate mutations is a convenient way to create bacterial mutations in the laboratory.

Toxigenicity in Corynebacterium diphtheriae is due to phage conversion.

If a bacterium carrying a plasmid that confers resistance to ampicillin is placed into medium without ampicillin,it may

When UV radiation damage occurs,DNA repair occurs only in the absence of template instruction.

Homologous recombination has been observed in

The designations Phe⁻,Leu⁻,and Ser⁺ refer to an organism's

Which process listed below does NOT require homologous recombination to maintain the new genetic material in the recipient cell?

You have performed the following mating experiment using Hfr and F⁻ strains of Escherichia coli: Hfr (thr⁺ leu⁺ gal⁺ strˢ)× F⁻ (thr⁻ leu⁻ gal⁻ strʳ).Which of the following selective media would you use to score recombinant colonies?

How might mutations in the sequence,whose enzyme is involved in DNA proofreading,be beneficial for a microbe in a highly competitive and dynamic community?

Which of the following methods may introduce foreign DNA into a recipient?

Which of the following features are common to transformation,transduction,and conjugation? (1)unidirectional transfer of genes (2)incomplete gene transfer (3)homologous recombination (4)meiosis occurring in the recipient

The positions of genes on a genetic map are marked according to

A typical mutation rate for a bacterium is in the range of 10⁻⁶ to 10⁻⁹ per kbp.

Consider the following experiment.First,large populations of two mutant strains of Escherichia coli are mixed,each requiring a different,single amino acid.After plating them onto a minimal medium,45 colonies grew.Which of the following may explain this result?

The evolutionary significance of phage conversion likely stems from an effective alteration of host cells.

The enzyme transposase may be coded for by insertion sequences on a

Plasmids that govern their own transfer are known as

Microorganisms and Microbiology

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