Exam 7: Metabolic Regulation

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Cyclic AMP (cAMP)is involved in the global regulation of catabolic pathways in Escherichia coli,including the lac operon,and a low activity of adenylate cyclase that makes cAMP is suggestive of catabolite repression.

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The function of a kinase is

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C

Defend why is it unsurprising that two-component regulatory systems are all but absent in bacteria that live at the expense of a host?

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Answers will vary,but a possible explanation is that an organism that has adapted to living inside of a host has such a narrow metabolic capacity that responses to nutrient limitation are less necessary than a bacterium growing in a dynamic community.

Proteins required at approximately the same level throughout a cell's growth cycle are often not subject to regulatory mechanisms and are constitutively synthesized.

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During chemotaxis,attractants increase the rate of autophosphorylation whereas repellants decrease this rate.

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A two-component regulatory system usually involves both the sensor and response proteins being subject to phosphorylation.

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How could you identify potential riboswitches with bioinformatics tools?

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Explain the function of cAMP in catabolite repression.

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The lac operon is an example of ________ control in which the presence of an ________ is required for transcription to occur.

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Some proteins that bind to DNA block transcription,whereas other proteins can activate transcription.

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Interpret the results to the following experiment.Transcriptional activity of chemotactic genes showed a high expression level during the following conditions: repelling away from compound 1,moving towards compound 2,and remaining sessile when presented compounds 3 and 4.

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Describe the basic two-component regulatory system,and compare the function of each component.

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Quorum sensing generally follows the mechanism of which type of regulation?

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Small molecules usually act directly (rather than indirectly)in regulating transcription.

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In most cases,the first product of a particular biosynthetic pathway represses the enzymes of the pathway.

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Regulation of an enzyme's activity occurs

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In catabolic repression,cells use the least abundant carbon source first.

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The most frequent way in which regulatory RNA molecules exert their effects is by

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Proteins and RNA molecules that are needed in the cell,at about the same level under all growth conditions,require constitutive expression.

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When arginine is added to a culture growing exponentially in a medium without arginine,what occurs?

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