Question 1

Which of the following is a mechanism of aminoglycosides?

Accepted Answer

A) inhibition of cell wall biosynthesis 
B) prevention of peptidoglycan crosslinking 
C) inhibition of DNA replication 
D) targeting the 30S ribosomal subunit 
A) inhibition of cell wall biosynthesis 
B) prevention of peptidoglycan crosslinking 
C) inhibition of DNA replication 
D) targeting the 30S ribosomal subunit

Question 2

Protein A has a strong affinity for

Accepted Answer

A) antigen. 
B) blood. 
C) protein. 
D) antibody. 
A) antigen. 
B) blood. 
C) protein. 
D) antibody.

Question 3

A positive antibody titer indicates

Accepted Answer

A) previous exposure to an antigen. 
B) active infection. 
C) latent disease. 
D) a healthy individual. 
A) previous exposure to an antigen. 
B) active infection. 
C) latent disease. 
D) a healthy individual.

Question 4

Discuss how recent advances in the molecular biology of nucleic acids have revolutionized methods used for the identification of infectious disease agents and how they are favored over traditional biochemical tests.

Accepted Answer

Molecular biology techniques that genera

Question 5

Literally hundreds of clinically useful EIAs have been developed.

Accepted Answer

A) True 
 B)False

Question 6

How is drug-resistant Neisseria gonorrhoeae treated?

Accepted Answer

A) with penicillinase-resistant β-lactams 
B) with tetracycline 
C) with synthetic analogs of tetracycline 
D) with vancomycin 
A) with penicillinase-resistant β-lactams 
B) with tetracycline 
C) with synthetic analogs of tetracycline 
D) with vancomycin

Question 7

Interferons promote viral replication.

Accepted Answer

A) True 
 B)False

Question 8

In the EIA test for HIV infection,the microtiter plate is coated with disrupted HIV particles.

Accepted Answer

A) True 
 B)False

Question 9

The oxygen-free gas commonly employed during anoxic incubation of a bacterial culture is a mixture of

Accepted Answer

A) nitrogen and carbon dioxide. 
B) nitrogen and carbon monoxide. 
C) nitrogen and argon. 
D) nitrogen and hydrogen. 
A) nitrogen and carbon dioxide. 
B) nitrogen and carbon monoxide. 
C) nitrogen and argon. 
D) nitrogen and hydrogen.

Question 10

Describe the three main components to PCR-based testing.

Accepted Answer

The first step requires that nucleic aci

Question 11

Latex bead agglutination tests are

Accepted Answer

A) rapid and reliable tests used for the detection of certain bacteria as well as certain serum antibodies. 
B) inferior tests used in developing countries not having facilities for more reliable assays. 
C) an integral part of the EIA test procedure. 
D) too expensive for routine use. 
A) rapid and reliable tests used for the detection of certain bacteria as well as certain serum antibodies. 
B) inferior tests used in developing countries not having facilities for more reliable assays. 
C) an integral part of the EIA test procedure. 
D) too expensive for routine use.

Question 12

Hydrogen sulfide production is assayed by growth in a medium containing ferric iron (Fe³⁺).

Accepted Answer

A) True 
 B)False

Question 13

Which of the following would be useful for treating Candida?

Accepted Answer

A) an azole 
B) a fusion inhibitor 
C) a macrolide 
D) a nucleoside reverse transcriptase inhibitor 
A) an azole 
B) a fusion inhibitor 
C) a macrolide 
D) a nucleoside reverse transcriptase inhibitor

Question 14

Which type of medium typically contains an indicator dye?

Accepted Answer

A) selective but not differential 
B) differential but not selective 
C) both selective and differential 
D) neither selective nor differential 
A) selective but not differential 
B) differential but not selective 
C) both selective and differential 
D) neither selective nor differential

Question 15

Hybridization requires a nucleic acid probe that binds to DNA or RNA of interest,which must be in the ________ form for hybridization to occur.

Accepted Answer

A) single-stranded 
B) double-stranded 
C) oligomerized 
D) polymerized 
A) single-stranded 
B) double-stranded 
C) oligomerized 
D) polymerized

Question 16

Propose a series of procedures to determine whether a patient has a urinary tract infection.

Accepted Answer

Direct microscopic examination of urine

Question 17

Gram stain smears of urethral exudates for the detection of Neisseria gonorrhoeae are highly specific.

Accepted Answer

A) True 
 B)False

Question 18

Bacteremia is a term used

Accepted Answer

A) to indicate the presence of bacteria in the blood. 
B) synonymously with septicemia. 
C) when the same organism is isolated from more than one site. 
D) to indicate the presence of bacteria in the lungs. 
A) to indicate the presence of bacteria in the blood. 
B) synonymously with septicemia. 
C) when the same organism is isolated from more than one site. 
D) to indicate the presence of bacteria in the lungs.

Question 19

One advantage of the fluorescent antibodies that permits disease diagnosis long before primary isolation techniques yield results is that

Accepted Answer

A) cross-reactions do not occur. 
B) few controls are required. 
C) nonspecific staining does not occur. 
D) it may be applied directly to tissue. 
A) cross-reactions do not occur. 
B) few controls are required. 
C) nonspecific staining does not occur. 
D) it may be applied directly to tissue.

Question 20

When using the EIA test for HIV screening,BOTH false negative and false positive results are possible.

Accepted Answer

A) True 
 B)False

Exam 27: Diagnostic Microbiology

Which of the following is a mechanism of aminoglycosides?

Protein A has a strong affinity for

A positive antibody titer indicates

Discuss how recent advances in the molecular biology of nucleic acids have revolutionized methods used for the identification of infectious disease agents and how they are favored over traditional biochemical tests.

Literally hundreds of clinically useful EIAs have been developed.

How is drug-resistant Neisseria gonorrhoeae treated?

Interferons promote viral replication.

In the EIA test for HIV infection,the microtiter plate is coated with disrupted HIV particles.

The oxygen-free gas commonly employed during anoxic incubation of a bacterial culture is a mixture of

Describe the three main components to PCR-based testing.

Latex bead agglutination tests are

Hydrogen sulfide production is assayed by growth in a medium containing ferric iron (Fe³⁺).

Which of the following would be useful for treating Candida?

Which type of medium typically contains an indicator dye?

Hybridization requires a nucleic acid probe that binds to DNA or RNA of interest,which must be in the ________ form for hybridization to occur.

Propose a series of procedures to determine whether a patient has a urinary tract infection.

Gram stain smears of urethral exudates for the detection of Neisseria gonorrhoeae are highly specific.

Bacteremia is a term used

One advantage of the fluorescent antibodies that permits disease diagnosis long before primary isolation techniques yield results is that

When using the EIA test for HIV screening,BOTH false negative and false positive results are possible.

Microorganisms and Microbiology

Microbial Cell Structure and Function

Microbial Metabolism

Molecular Biology

Microbial Growth and Control

Microbial Genomics

Metabolic Regulation

Viruses and Virology

Viral Genomes and Diversity

Genetics of Bacteria and Archaea

Genetic Engineering and Biotechnology

Microbial Evolution and Systematics

Metabolic Diversity of Microorganisms

Functional Diversity of Bacteria

Diversity of Bacteria

Diversity of Archaea

Diversity of Eukaryotic Microorganisms

Methods in Microbial Ecology

Microbial Ecosystems

Nutrient Cycles

Microbiology of the Built Environment

Microbial Symbioses

Microbial Interactions With Humans

Immunity and Host Defense

Immune Mechanisms

Molecular Immunology

Epidemiology

Person-To-Person Bacterial and Viral Diseases

Vectorborne and Soilborne Bacterial and Viral Diseases

Water and Food As Vehicles of Bacterial Diseases

Eukaryotic Pathogens: Fungal and Parasitic Diseases

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