Question 1

An individual who contains cells that are mutated is called a chimera.

Accepted Answer

A) True 
 B)False  False

Question 2

Which of the following terms represents a cell that can form any other cell of the organism?

Accepted Answer

A) Pluripotent 
B) Totipotent 
C) Unipotent 
A) Pluripotent 
B) Totipotent 
C) Unipotent B

Question 3

A transgenic organism has recombinant DNA integrated into its genome.

Accepted Answer

A) True 
 B)False  True

Question 4

What is the purpose of the cyanogen bromide (CNBr) in the manufacture of insulin and somatostatin by recombinant bacteria?

Accepted Answer

A) Allows for the uptake of the plasmid 
B) Enhances the activity of the restriction enzymes 
C) Creates sticky ends for integration into the plasmid 
D) Cleaves the protein from the b-galactosidase protein 
A) Allows for the uptake of the plasmid 
B) Enhances the activity of the restriction enzymes 
C) Creates sticky ends for integration into the plasmid 
D) Cleaves the protein from the b-galactosidase protein

Question 5

Although Dolly was only three years old, her chromosomes had the length of a 9-10 year old sheep. Which of the following best describes why this occurred?

Accepted Answer

A) The cell lines that created Dolly were aged prematurely in the lab 
B) Nonhomologous recombination 
C) A mutation enhanced the rate of aging 
D) Dolly was not a clone 
E) The telomeres of the somatic cells that Dolly originated from were shortening 
A) The cell lines that created Dolly were aged prematurely in the lab 
B) Nonhomologous recombination 
C) A mutation enhanced the rate of aging 
D) Dolly was not a clone 
E) The telomeres of the somatic cells that Dolly originated from were shortening

Question 6

Using the NeoR/Tk system of introducing gene replacements into mice (as described in the text), which of the following would identify those mouse cells that were the result of homologous recombination?

Accepted Answer

A) NeoR only 
B) Tk only 
C) NeoR and Tk 
D) Neither NeoR or Tk 
A) NeoR only 
B) Tk only 
C) NeoR and Tk 
D) Neither NeoR or Tk

Question 7

Why was the addition of methionine necessary during the production of somatostatin by recombinant bacteria?

Accepted Answer

A) So that it was compatible with the restriction enzyme 
B) As a selectable marker 
C) To allow the somatostatin to link to a bacterial protein 
D) To increase the length of the peptide to make it more commercial 
A) So that it was compatible with the restriction enzyme 
B) As a selectable marker 
C) To allow the somatostatin to link to a bacterial protein 
D) To increase the length of the peptide to make it more commercial

Question 8

Bioremediation has been used to treat which of the following?

Accepted Answer

A) Oil spills 
B) Sewage 
C) Pesticides 
D) Heavy metals 
E) All of the answers have been successfully remediated 
A) Oil spills 
B) Sewage 
C) Pesticides 
D) Heavy metals 
E) All of the answers have been successfully remediated

Question 9

In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.

Accepted Answer

A) True 
 B)False

Question 10

An organism that can be regenerated by somatic cells is called multipotent.

Accepted Answer

A) True 
 B)False

Question 11

Which of the following is an example of a pluripotent cell?

Accepted Answer

A)  embryonic stem cells 
B)  red blood cells 
C)  fetal heart cells 
D)  umbilical cord blood 
E)  nerve cells 
A)  embryonic stem cells 
B)  red blood cells 
C)  fetal heart cells 
D)  umbilical cord blood 
E)  nerve cells

Question 12

The first human diseases that was successfully treated using gene therapy was ______.

Accepted Answer

A)  Diabetes 
B)  Cystic fibrosis 
C)  Severe combined immunodeficiency diseases 
D)  Muscular dystrophy 
A)  Diabetes 
B)  Cystic fibrosis 
C)  Severe combined immunodeficiency diseases 
D)  Muscular dystrophy

Question 13

Unipotent cells may differentiate into all other cell types of the body.

Accepted Answer

A) True 
 B)False

Question 14

Which of the following mechanisms uses high-velocity microprojectiles, coated with DNA, to produce a transgenic plant?

Accepted Answer

A) A.tumefaciens 
B) Electroporation 
C) Microinjection 
D) Biolistic gene transfer 
A) A.tumefaciens 
B) Electroporation 
C) Microinjection 
D) Biolistic gene transfer

Question 15

A major disadvantage with current gene therapy techniques using viral vectors is the possibility of an immune response against the virus.

Accepted Answer

A) True 
 B)False

Question 16

Hematopoietic stem cells are _________.

Accepted Answer

A) Pluripotent 
B) Totipotent 
C) Unipotent 
D) Multipotent 
A) Pluripotent 
B) Totipotent 
C) Unipotent 
D) Multipotent

Question 17

In the production of transgenic plants, what selectable marker is most commonly used to indicate that a cell has taken up the T DNA that includes the cloned gene?

Accepted Answer

A) Ampicillin 
B) Neomycin 
C) Streptomycin 
D) Kanamycin 
A) Ampicillin 
B) Neomycin 
C) Streptomycin 
D) Kanamycin

Question 18

Identical twins are an example of gene cloning.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following is not an advantage of a transgenic plant?

Accepted Answer

A) Widely accepted by the public 
B) Increased resistance to disease and pests 
C) Adaptability to harsh environments 
D) Improved shelf life 
A) Widely accepted by the public 
B) Increased resistance to disease and pests 
C) Adaptability to harsh environments 
D) Improved shelf life

Question 20

The first organism ever cloned by researchers was Dolly the sheep in 1997.

Accepted Answer

A) True 
 B)False

Exam 21: Biotechnology

An individual who contains cells that are mutated is called a chimera.

Which of the following terms represents a cell that can form any other cell of the organism?

A transgenic organism has recombinant DNA integrated into its genome.

What is the purpose of the cyanogen bromide (CNBr) in the manufacture of insulin and somatostatin by recombinant bacteria?

Although Dolly was only three years old, her chromosomes had the length of a 9-10 year old sheep. Which of the following best describes why this occurred?

Using the NeoR/Tk system of introducing gene replacements into mice (as described in the text), which of the following would identify those mouse cells that were the result of homologous recombination?

Why was the addition of methionine necessary during the production of somatostatin by recombinant bacteria?

Bioremediation has been used to treat which of the following?

In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.

An organism that can be regenerated by somatic cells is called multipotent.

Which of the following is an example of a pluripotent cell?

The first human diseases that was successfully treated using gene therapy was ______.

Unipotent cells may differentiate into all other cell types of the body.

Which of the following mechanisms uses high-velocity microprojectiles, coated with DNA, to produce a transgenic plant?

A major disadvantage with current gene therapy techniques using viral vectors is the possibility of an immune response against the virus.

Hematopoietic stem cells are _________.

In the production of transgenic plants, what selectable marker is most commonly used to indicate that a cell has taken up the T DNA that includes the cloned gene?

Identical twins are an example of gene cloning.

Which of the following is not an advantage of a transgenic plant?

The first organism ever cloned by researchers was Dolly the sheep in 1997.

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and Bacteriophages

Variation in Chromosome Structure and Number

Molecular Structure of DNA and RNA

Chromosome Organization and Molecular Structure

DNA Replication

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional Regulation

Gene Regulation in Eukaryotes II: Epigenetics and Regulation at the RNA Level

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular Level

DNA Technologies

Genomics I: Analysis of DNA

Genomics II: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

Filters

Using the Neo^R/Tk system of introducing gene replacements into mice (as described in the text), which of the following would identify those mouse cells that were the result of homologous recombination?