Exam 11: DNA Replication

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You have isolated what appears to be alien DNA. While studying its replication, you performed the exact experiment Meselson and Stahl did. After three generations, the DNA is subjected to a CsCl gradient and only one band appears. What type of replication does this DNA undergo?

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C

DNA polymerase III has an error in replication once every 100 million nucleotides.

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True

Which of the following best describes the mechanism of DNA replication in which both a parental strand and daughter strand are combined following replication?

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B

Bacterial DNA has how many origins of replication?

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You have discovered a strain of

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DNA polymerase III is the main replication enzyme in prokaryotes. What is it in eukaryotes?

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Which of the following is a restriction placed on DNA polymerase?

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The ability of the DNA polymerase to remove mismatched bases is called exonuclease cleavage (proofreading).

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DNA polymerase is a primer-dependent enzyme that functions only in the 5'-3' direction. These are the two most fundamental concepts to understanding this enzyme. Based on this, which of the following enzyme pairs are analogous in prokaryotes and eukaryotes?

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The movement of the replication fork in bacterial replication is unidirectional.

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Catenanes can only form in cells with circular chromosomes, such as bacteria.

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DNA polymerase III can synthesize a new strand of DNA at the rate of 300 nucleotides per minute.

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Fills in small regions of DNA where the RNA primers were located.

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Which of the following best describes the mechanism of DNA replication in which both parental strands remain together following replication?

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Which of the following is not correct concerning the initiation of bacterial replication?

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Which of the following is an example of a processive enzyme?

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Synthesizes the lagging strand of the DNA.

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What functions are accomplished by the primosome?

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DNA polymerase III has the ability to begin synthesis of the new daughter strands immediately following the formation of the replication fork.

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Which of the following best describes the double-helix of DNA?

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