Exam 15: Gene Regulation in Eukaryotes I: Transcriptional and Translation Regulation

Select all structural motifs that promote protein dimerization.

Methylation of DNA bases is one way that regulatory transcription factors may influence gene expression.

Insulators exert their effects on gene expression through histone acetyltransferase, histone deacetylase, and RNA polymerase.

A repressor protein would enhance the ability of TFIID to bind to the TATA box of the promoter.

A mutation in which of the following would result in little or no effect on the expression of a gene regulated by a CRE?

CpG islands are associated with which of the following?

Genomic imprinting is a result of ________.

Activator proteins bind to silencer sequences and repressor proteins bind to enhancer sequences.

What general transcription factor is most often affected by regulatory transcription factors?

DNA methylation usually activates gene expression.

A heterodimer occurs when two identical transcription factors interact on a sequence of DNA.

Housekeeping genes are unmethylated and active in most cells.

Prior to hormone binding, receptors for steroid hormones are usually found in the nucleus of the cell.

If a portion of a transcription factor's domain is the same in a variety of organisms, it is called a motif.

DNA that contains actively transcribed genes would most likely contain chromatin in the closed configuration.

Which of the following might complicate the interpretation of ChIP-Seq data?

Which DNA sequence would also work in place of the following bi-directional enhancer? 5' GTTC 3' 3' CAAG 5'

A particular gene has a mutation in its NFR that causes it to bind abnormally tightly to histones. What effect would you expect this mutation has on the expression of this gene?

Nucleosome location may be changed by a process called ATP-dependent chromatin remodeling.

In ChIP-Seq, the DNA fragments to be sequenced are around 150 bp in length. What does this size of DNA represent?