Question 1

Interrupted mating is defined as ________.

Accepted Answer

A)  the inability of a bacteriophage to transfer genetic material 
B)  a mechanism of transformation 
C)  a process of asexual reproduction in bacteria 
D)  a process by which conjugation is stopped using a blender 
A)  the inability of a bacteriophage to transfer genetic material 
B)  a mechanism of transformation 
C)  a process of asexual reproduction in bacteria 
D)  a process by which conjugation is stopped using a blender D

Question 2

Which of the following mechanisms of genetic transfer utilizes a physical connection between two bacteria?

Accepted Answer

A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct 
A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct C

Question 3

A cell with an F factor integrated into the bacterial chromosome is called ________.

Accepted Answer

A)  Hfr 
B)  F+ 
C)  Conjugative plasmid 
A)  Hfr 
B)  F+ 
C)  Conjugative plasmid A

Question 4

Which of the following is true regarding conjugation?

Accepted Answer

A)  The bacteria must be in physical contact. 
B)  Most bacterial species have the ability to conjugate. 
C)  Strains called donor strains can transfer genetic information. 
D)  Donor strains can convert non-donor strains into donor strains. 
E)  All of the answers are correct. 
A)  The bacteria must be in physical contact. 
B)  Most bacterial species have the ability to conjugate. 
C)  Strains called donor strains can transfer genetic information. 
D)  Donor strains can convert non-donor strains into donor strains. 
E)  All of the answers are correct.

Question 5

Which of the following matings would have the highest frequency of chromosomal gene transfer?

Accepted Answer

A)  F+ x F- 
B)  F' x F- 
C)  Hfr x F- 
A)  F+ x F- 
B)  F' x F- 
C)  Hfr x F-

Question 6

The units of genetic distance derived from bacterial conjugation studies are called ________.

Accepted Answer

A)  seconds 
B)  map units 
C)  centimorgans 
D)  recombination units 
E)  minutes 
A)  seconds 
B)  map units 
C)  centimorgans 
D)  recombination units 
E)  minutes

Question 7

Cotransduction establishes the general order of genes on a bacterial chromosome, while conjugation is more specific and thus may be used to establish precise distances.

Accepted Answer

A) True 
 B)False

Question 8

The site on a plasmid that is digested, producing a single strand of DNA, to allow conjugation to occur is called the ________.

Accepted Answer

A)  origin of transfer 
B)  relaxase 
C)  nucleoprotein 
D)  T DNA 
A)  origin of transfer 
B)  relaxase 
C)  nucleoprotein 
D)  T DNA

Question 9

A phage that can carry a larger piece of a bacterial chromosome would have what type of effect on cotransduction frequencies?

Accepted Answer

A)  Raise the cotransduction frequency. 
B)  Lower the cotransduction frequency. 
C)  Have no effect on the cotransduction frequency. 
A)  Raise the cotransduction frequency. 
B)  Lower the cotransduction frequency. 
C)  Have no effect on the cotransduction frequency.

Question 10

The process of conjugation may introduce new alleles into the recipient strain, altering its genotype.

Accepted Answer

A) True 
 B)False

Question 11

A histidine, methionine auxotroph ________.

Accepted Answer

A)  can synthesize only histidine and methionine 
B)  can only use histidine and methionine as nutrition sources 
C)  cannot synthesize histidine and methionine 
D)  is killed by histidine and methionine 
E)  lacks proteins with histidine or methionine 
A)  can synthesize only histidine and methionine 
B)  can only use histidine and methionine as nutrition sources 
C)  cannot synthesize histidine and methionine 
D)  is killed by histidine and methionine 
E)  lacks proteins with histidine or methionine

Question 12

The process of interrupted mating may be used to do which of the following?

Accepted Answer

A)  Inhibit transduction 
B)  Convert the recipient cell to F+ 
C)  Determine the relative position of bacterial genes 
D)  Alter the genotype of the donor cell 
A)  Inhibit transduction 
B)  Convert the recipient cell to F+ 
C)  Determine the relative position of bacterial genes 
D)  Alter the genotype of the donor cell

Question 13

Which of the following mechanisms of genetic transfer was first observed by Lederberg and Tatum (1946) in Escherichia coli?

Accepted Answer

A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct 
A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct

Question 14

In the Lederberg and Tatum experiment what is the purpose of performing the experiment with the strains without adding them together?

Accepted Answer

A)  It serves as a control to calculate the rate of reversion mutations. 
B)  There is no experimental purpose to performing that part of the experiment. 
C)  It tests that the strains were made correctly. 
D)  It tests to make sure the media was made correctly. 
A)  It serves as a control to calculate the rate of reversion mutations. 
B)  There is no experimental purpose to performing that part of the experiment. 
C)  It tests that the strains were made correctly. 
D)  It tests to make sure the media was made correctly.

Question 15

A minute is the basic unit of map distance in bacteria.

Accepted Answer

A) True 
 B)False

Question 16

Which of the following is true regarding bacterial genetics?

Accepted Answer

A)  Bacteria are usually diploid organisms. 
B)  Bacteria primarily reproduce sexually. 
C)  The patterns of inheritance in bacteria are studied using different mechanisms than eukaryotes. 
D)  Bacteria generally have linear chromosomes. 
A)  Bacteria are usually diploid organisms. 
B)  Bacteria primarily reproduce sexually. 
C)  The patterns of inheritance in bacteria are studied using different mechanisms than eukaryotes. 
D)  Bacteria generally have linear chromosomes.

Question 17

A structure called a(n) ________ allows the transfer of genetic information during conjugation.

Accepted Answer

A)  F factor 
B)  plasmid 
C)  sex pilus 
D)  transformation bridge 
A)  F factor 
B)  plasmid 
C)  sex pilus 
D)  transformation bridge

Question 18

Acquired antibiotic resistance between two different species is an example of vertical gene transfer.

Accepted Answer

A) True 
 B)False

Question 19

What are the mechanisms by which bacteria preferentially take up DNA from individuals of the same species? Check all that apply.

Accepted Answer

A)  A specific sequence in the species DNA allows for uptake of DNA fragments that carry that sequence. 
B)  Proteins that are secreted by the bacteria must reach a threshold level that is correlated to the number of individuals of the same species being present. 
C)  Some species require a specific calcium level for uptake and the levels of calcium are correlated with the number of bacteria in localized area. 
A)  A specific sequence in the species DNA allows for uptake of DNA fragments that carry that sequence. 
B)  Proteins that are secreted by the bacteria must reach a threshold level that is correlated to the number of individuals of the same species being present. 
C)  Some species require a specific calcium level for uptake and the levels of calcium are correlated with the number of bacteria in localized area.

Question 20

Which of the following uses a bacteriophage as an intermediary for the genetic information?

Accepted Answer

A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct 
A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct

Exam 7: Genetic Transfer and Mapping in Bacteria

Interrupted mating is defined as ________.

Which of the following mechanisms of genetic transfer utilizes a physical connection between two bacteria?

A cell with an F factor integrated into the bacterial chromosome is called ________.

Which of the following is true regarding conjugation?

Which of the following matings would have the highest frequency of chromosomal gene transfer?

The units of genetic distance derived from bacterial conjugation studies are called ________.

Cotransduction establishes the general order of genes on a bacterial chromosome, while conjugation is more specific and thus may be used to establish precise distances.

The site on a plasmid that is digested, producing a single strand of DNA, to allow conjugation to occur is called the ________.

A phage that can carry a larger piece of a bacterial chromosome would have what type of effect on cotransduction frequencies?

The process of conjugation may introduce new alleles into the recipient strain, altering its genotype.

A histidine, methionine auxotroph ________.

The process of interrupted mating may be used to do which of the following?

Which of the following mechanisms of genetic transfer was first observed by Lederberg and Tatum (1946) in Escherichia coli?

In the Lederberg and Tatum experiment what is the purpose of performing the experiment with the strains without adding them together?

A minute is the basic unit of map distance in bacteria.

Which of the following is true regarding bacterial genetics?

A structure called a(n) ________ allows the transfer of genetic information during conjugation.

Acquired antibiotic resistance between two different species is an example of vertical gene transfer.

What are the mechanisms by which bacteria preferentially take up DNA from individuals of the same species? Check all that apply.

Which of the following uses a bacteriophage as an intermediary for the genetic information?

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional and Translation Regulation

Gene Regulation in Eukaryotes Ii: Epigenetics

Non-Coding Rnas

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination, Immunogenetics, and Transposition

Molecular Technologies

Biotechnology

Genomics I: Analysis of Dna

Genomics Ii: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Complex and Quantitative Traits

Evolutionary Genetics

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